FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800806

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800806
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20909670
Sequences flagged as poor quality0
Sequence length65
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCCGTCTTCTGCTTG354860.16971095191841862TruSeq Adapter, Index 18 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG44400.050.08885248
CTCGTAT45950.048.2066744
GATCGGA46150.048.0889471
CGTATGC47400.047.10534746
TCTCGTA47650.046.61060743
TCGTATG49150.045.3681445
TGCCGTC49550.044.70426650
ATCGGAA50100.044.2548752
ATGCCGT50750.043.76346249
GCCGTCT50500.043.74648351
ATCTCGT51250.043.27894642
TCGGAAG52450.042.374093
ACACGTC53450.041.9391513
CGTCTGA54400.041.0983216
CGTCTTC56300.039.3969153
CCGTCTT56550.039.0662752
CGGAAGA57500.038.75524
ACGTCTG59050.037.91199515
AATCTCG61150.036.46515341
CACGTCT62400.036.0657314
Produced by FastQC (version 0.11.5)