FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800812

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800812
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29031636
Sequences flagged as poor quality0
Sequence length65
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGAACAAATCTCGTATGCCGTCTTCTGCTTG291320.10034570562954151TruSeq Adapter, Index 10 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG41050.042.3249948
GATCGGA42050.041.770931
CTCGTAT43750.039.6439444
CGTATGC44250.039.39596646
TCTCGTA44850.038.4085543
ATGCCGT45650.038.12564549
TCGTATG47100.037.45051645
TGCCGTC47650.036.58838750
ATCGGAA49900.035.2945332
GCCGTCT49900.035.05684351
ATCTCGT49550.035.0034842
CGTCTGA53800.032.67695216
TCGGAAG54950.031.9956153
ACACGTC55350.031.925613
CGTCTTC56850.031.03054653
CCGTCTT58400.030.10593252
CGGAAGA60400.028.913144
ACGTCTG64250.027.49990815
AATCTCG66600.026.21953641
CACGTCT68600.025.97110214
Produced by FastQC (version 0.11.5)