FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800813

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800813
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22076403
Sequences flagged as poor quality0
Sequence length65
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG34700.037.23423848
CTCGTAT35000.036.07227744
GATCGGA37100.035.708091
TCTCGTA36650.034.68966743
TCGTATG37300.034.55973445
CGTATGC38300.033.50335746
ATCGGAA42900.030.8065552
ATCTCGT41850.030.37936442
ATGCCGT42850.030.29002649
ACACGTC47450.027.0426913
TGCCGTC50650.025.39246750
CGTCTGA52100.024.91218416
TCGGAAG54050.024.5079233
GCCGTCT54050.024.06831451
CGTCTTC55500.023.55103153
CCGTCTT55650.023.37632252
ACGTCTG55800.023.3660315
CGGAAGA58400.022.6822644
GTATGCC58600.022.60198247
CACGTCT60750.021.80202914
Produced by FastQC (version 0.11.5)