FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800814

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800814
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27683324
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATAT289300.10450334649119447No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATCG17650.020.05571658
AACGCTA34500.019.06725523
GTCCGTT35250.018.7514151
ACGCTAC36100.018.54902524
GTCCGGT37000.018.0240291
CGCTACC39150.017.10395225
CTATCGG21550.016.5966959
CTACACG40800.016.3399257
GTTCCTA51200.016.01708615
GCCCTAA45100.015.95962852
CCCGTAT24600.015.9537111
AACGAAT40550.015.34946339
GTCGGTT26900.015.2478181
ACGAATT40900.015.07386440
GTCCGCT40800.015.0434831
CCTAAGC50600.014.69127554
CTCCGTT43300.014.651961
CTCGGTT74700.014.5764231
GTCCGAT20100.014.5339721
CCCCGCA214850.014.48500735
Produced by FastQC (version 0.11.5)