FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16014689
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC338420.21131849641288694No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC86650.030.8471244
GACCGCG85400.030.8144135
CAGACCG87750.030.628543
ACCGCGT88750.029.8155276
CGCGTTC89900.029.4333938
CCGCGTT90550.029.2876327
TTCGACG16400.028.0607436
TCAGACC106500.026.4551472
GTTCGAC18100.025.263885
CGACGAG19050.023.6921278
CTCAGAC127450.023.4176391
TCGACGA20250.023.0168067
GCGTTCT117100.022.7468269
TACGGAG116650.022.45568534
AATACGG118350.022.43292632
ATACGGA119800.022.1114733
CGTTCTC129100.021.36364410
GAACGAT126900.021.10699545
CGGTATA27650.021.01751520
CAATACG129250.020.97467431
Produced by FastQC (version 0.11.5)