FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21249938
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC1100500.5178838639435089No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGA287970.13551568950459997No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTG213000.10023558656971139No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG19150.032.6567966
CAGACCG252400.031.8042013
AGACCGC252450.031.71614
GACCGCG251250.031.6551845
ACCGCGT258750.030.8271086
TCAGACC279650.029.8655382
CGCGTTC273550.029.1806168
CCGCGTT273500.029.1538037
CTCAGAC299300.028.9701861
TCGACGA24400.025.9929227
GCGTTCT311900.025.7149099
CGTTCTC335650.024.88850210
CGGTATA36000.024.17297420
GTTCGAC26750.023.3799235
CGACGAG26000.023.2586178
AATACGG363650.021.90266232
ATACGGA366150.021.76856233
TACGGAG361450.021.74149534
CCTCTCA380250.021.59805719
ACGGAGA375600.020.91447335
Produced by FastQC (version 0.11.5)