FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33289241
Sequences flagged as poor quality0
Sequence length65
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC614520.1846001835848405No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT132900.030.1670066
AGACCGC134950.029.8617694
CAGACCG136350.029.6417033
CCGCGTT141200.028.3710487
GACCGCG141800.028.31525
CGCGTTC143400.027.9358318
TTCGACG27700.027.2645236
TCAGACC172850.024.7657092
TCGACGA33800.022.3425947
GCGTTCT187200.021.6201769
CTCAGAC213150.021.5449121
GTTCGAC34650.021.5404625
CGGTATA42050.021.46678720
CGACGAG36000.020.403658
ATACGGA202550.020.1427433
TACGGAG203250.019.94273834
AATACGG211000.019.23820732
GAACGAT215350.018.97294845
CAATACG221900.018.6918731
AACGATC220850.018.59395446
Produced by FastQC (version 0.11.5)