FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24628401
Sequences flagged as poor quality0
Sequence length65
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA31450.036.494911
TATGCCG33100.034.7564248
CGTATGC33900.034.02322846
GTGCGAA33850.033.72524635
CTCGTAT35050.032.40194744
TCTCGTA35250.032.30179243
TCGTATG36100.031.86807345
GCGAAAT36250.031.73620437
TGCGAAA38000.030.50786236
CGGTGCG38550.029.61347633
ATGCCGT39750.029.16445249
ATCGGAA39850.029.0185992
ATCTCGT41200.027.92323742
CGTCTGA42850.027.32990316
ACACGTC43950.026.24316813
TGCCGTC45450.025.70157450
GTCACGG45000.025.69643429
ACGGTGC45100.025.57425932
TCACGGT45400.025.4702430
AGTCACG46000.025.4584528
Produced by FastQC (version 0.11.5)