FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800823

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800823
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21463908
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC631260.29410301236848385No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC140200.031.3633174
GACCGCG139600.031.0961915
CGCGTTC141050.030.9905368
CAGACCG145350.030.4148793
ACCGCGT145300.030.0037086
CCGCGTT146850.029.7264297
TTCGACG15950.029.2188116
TCAGACC171900.027.1262442
CTCAGAC193150.025.6013371
AATACGG180650.023.98522832
GCGTTCT184950.023.7143159
TACGGAG180800.023.67195134
ATACGGA184350.023.50372533
GTTCGAC20500.023.1924885
CGTTCTC201750.022.87993210
CAATACG194350.022.59801131
GAACGAT195450.022.42539845
ACGGAGA196550.021.94003935
AACGATC202450.021.8102746
CCCAATA208600.021.46458829
Produced by FastQC (version 0.11.5)