FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25915173
Sequences flagged as poor quality0
Sequence length65
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTCCTGAATCTCGTATGCCGTCTTCTGCTTG1032400.39837665756659235TruSeq Adapter, Index 23 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA119550.054.868571
TATGCCG120900.053.9466748
CTCGTAT120250.053.84577644
CGTATGC122500.053.3624646
ATCGGAA123650.053.0448272
TCTCGTA122150.052.8874743
TCGTATG123800.052.68297645
TGCCGTC128450.050.7987750
ATCTCGT127500.050.64513842
ATGCCGT129000.050.58218849
ACACGTC129750.050.5399813
TCGGAAG130200.050.5302353
CGTCTGA129350.050.42338616
GCCGTCT130000.050.26116651
CGGAAGA135950.048.3496634
CCGTCTT136300.048.0245952
ACGTCTG136400.047.9245915
CGTCTTC137300.047.6103553
CACGTCT142950.045.8112214
AATCTCG146150.044.1823841
Produced by FastQC (version 0.11.5)