FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12642434
Sequences flagged as poor quality0
Sequence length65
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC288980.22857940171963723No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG19700.034.8914646
GTTCGAC21450.032.3196535
AGACCGC69650.031.8509794
CAGACCG70600.031.5895333
GACCGCG70050.031.33225
CGCGTTC72900.030.3897028
ACCGCGT73000.030.2683326
CGGTATA30400.029.69341920
TCGACGA23700.029.1260497
CCGCGTT77250.028.7930037
TCAGACC85000.027.7302592
CGACGAG25200.027.2752958
AGTTCGA28850.024.9499744
AATACGG90400.024.08252332
AACGACG16900.023.91357416
TACGGAG89850.023.90181234
CTCAGAC105400.023.8917081
ATACGGA91850.023.6381133
CAGTTCG32100.022.9752923
ATAGAGT41950.022.924424
Produced by FastQC (version 0.11.5)