FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20950153
Sequences flagged as poor quality0
Sequence length65
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGATTGCATCTCGTATGCCGTCTTCTGCTTG358150.17095340544768337TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA52250.044.04511
TATGCCG53300.042.4487848
ACCGGAT54000.041.8443932
CTCGTAT54350.041.5744344
CGTATGC55050.041.1529546
TCGTATG55550.040.94184545
CGGATTG56750.040.02461234
TCTCGTA56650.039.8344343
ATGCCGT59200.038.6168749
ATCGGAA59850.038.4453772
ATCTCGT60750.036.85467542
CCGGATT62100.036.48143433
ACACGTC63600.036.1771713
CACCGGA64800.034.96137231
CGTCTGA66000.034.77224716
TGCCGTC67900.033.7123550
TCACCGG68000.033.57641630
TCGGAAG69050.033.4107933
CATCTCG68250.033.02081741
CCGTCTT72100.031.54463652
Produced by FastQC (version 0.11.5)