FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20341783
Sequences flagged as poor quality0
Sequence length65
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATAT305950.15040471132741903No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA22250.024.65966423
ACGCTAC23700.023.39988924
CGCTACC26200.021.39225425
GCCCTAA42100.020.24991452
CCTAAGC42850.019.82664154
ACTATCG12300.019.42598558
CTACACG30900.018.90209257
GTCCGTT18800.018.8347571
AGCCCTA50000.018.46638151
CCCTAAG46900.018.1774353
GTCGGTT22900.017.9108411
GTTCCTA35400.017.74940515
CCCGTAT17850.017.5228311
AAAACGC32400.017.38976321
GCACTAC34250.017.311725
CCCCCGC170100.016.97780634
CGAGGCG34800.016.86868754
AAGCGGC167700.016.69311528
CCCCGCA169950.016.55885935
CTCGGTT66550.016.538551
Produced by FastQC (version 0.11.5)