FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27078751
Sequences flagged as poor quality0
Sequence length65
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATGTACATCTCGTATGCCGTCTTCTGCTTG287278610.609004824483966TruSeq Adapter, Index 2 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA3066950.058.8423231
ATCGGAA3086400.058.5692182
TCGGAAG3087100.058.5064853
ACACGTC3093450.058.44264613
CGGAAGA3090950.058.3773084
CGTCTGA3091800.058.3650716
ACGTCTG3098000.058.27301815
CTCGTAT3077950.058.2320244
CACGTCT3104700.058.22232414
TATGCCG3091150.058.2207448
GCACACG3106600.058.20000511
TCTCGTA3079450.058.19376443
CGTATGC3095750.058.1769146
TCGTATG3099700.058.13026445
CACACGT3112700.058.10585812
ATGCCGT3097300.058.10238649
ATCTCGT3085600.058.09020642
GCCGTCT3096750.058.0879451
TGCCGTC3099400.058.04017650
CATCTCG3070200.058.00228541
Produced by FastQC (version 0.11.5)