FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800830
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23626296
Sequences flagged as poor quality0
Sequence length65
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTGCGAAATCTCGTATGCCGTCTTCTGCTTG2756451.1666873216182512TruSeq Adapter, Index 9 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGAA297700.057.3263835
CGGTGCG298950.057.0433
GATCGGA300950.057.0266761
TATGCCG301450.057.0108648
CTCGTAT300600.056.9036244
CGTATGC302150.056.88698246
TCTCGTA302600.056.55844543
GCGAAAT302100.056.51180637
TCGTATG305600.056.3200845
ATCGGAA306100.056.1844062
TGCGAAA304950.056.02152336
ATGCCGT307300.055.91678649
TGCCGTC307550.055.8617450
ACACGTC308900.055.83252713
GTCACGG305600.055.81737529
TCACGGT306200.055.7078930
ACGGTGC306250.055.6707232
GCCGTCT308750.055.6350851
TCGGAAG309650.055.592513
ATCTCGT308150.055.5302142
Produced by FastQC (version 0.11.5)