FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19567529
Sequences flagged as poor quality0
Sequence length65
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTCACCATCTCGTATGCCGTCTTCTGCTTG311260.15906965054197697TruSeq Adapter, Index 1 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA40100.050.2226071
TATGCCG42850.047.2241648
CTCGTAT44300.045.54387344
ATCGGAA45700.044.418642
CGTATGC45900.044.2133446
TCTCGTA45500.044.01857443
TGCCGTC46400.043.61132450
ATGCCGT47150.043.04273649
TCGTATG47900.042.42885645
GCCGTCT48100.042.1926251
CGTCTGA48400.041.8686516
TCGGAAG48900.041.5671353
ACACGTC49850.040.47453313
ATCTCGT50300.040.22848542
CATCTCG50800.039.2518941
CGTCTTC53600.038.08397353
ACGTCTG53500.037.76716615
CCGTCTT53900.037.7625452
CGGAAGA54100.037.571774
CACGTCT57500.035.1398814
Produced by FastQC (version 0.11.5)