FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800832

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800832
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17028621
Sequences flagged as poor quality0
Sequence length65
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG14600.031.1164256
AGACCGC40600.028.0462914
GACCGCG41900.027.1056355
CAGACCG43600.026.5901173
CGCGTTC44450.025.417848
ACCGCGT45250.025.164636
GTTCGAC18550.023.694925
CCGCGTT47950.023.6240437
CTACACG55550.022.94141657
AACGCTA56100.022.76814823
ACGCTAC56050.022.7358324
TCGACGA20550.021.819437
CGCTACC59450.021.33630825
CGGTATA25550.021.01276220
CGACGAG21700.019.575578
ACACGTT64400.019.42229359
AAAACGC66300.019.39883421
TACACGT64750.019.3628758
TCAGACC64150.019.3598562
GAAAACG71400.017.93057620
Produced by FastQC (version 0.11.5)