FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800835

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800835
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19410939
Sequences flagged as poor quality0
Sequence length65
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTTCGAATCTCGTATGCCGTCTTCTGCTTG766790.3950298334356725TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG90600.054.53221548
GATCGGA91850.054.1188161
CGTATGC92250.053.62079246
CTCGTAT93150.053.19771244
TCTCGTA94000.052.90535443
CGAATCT94650.052.29393439
TCGTATG94750.052.20599445
ATGCCGT94300.052.2048949
ACTTCGA95150.052.05187635
ATCGGAA96550.051.4865262
TGCCGTC95800.051.32590550
GCCGTCT96200.051.23513451
ACACGTC97650.051.01819213
TCGGAAG97550.050.955843
ATCTCGT97750.050.7554242
CGTCTGA99050.050.32686616
CACTTCG99150.049.86873634
CTTCGAA101050.049.0405336
TCGAATC101150.048.81682238
CGGAAGA101900.048.664794
Produced by FastQC (version 0.11.5)