FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800836

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800836
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26322855
Sequences flagged as poor quality0
Sequence length65
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTAGAATCTCGTATGCCGTCTTCTGCTTG19373167.359824760650014TruSeq Adapter, Index 20 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA2121650.058.791311
ATCGGAA2140400.058.4726682
TCGGAAG2139450.058.3952333
CGTCGTA2109000.058.3216433
ACGTCGT2115250.058.3076332
ACACGTC2152600.058.301613
CGGAAGA2142400.058.2418484
CACGTCG2115100.058.22506331
CGTCTGA2149900.058.2115516
GTCGTAG2109600.058.2042534
TATGCCG2148600.058.12264348
ACGTCTG2155050.058.09707615
CTCGTAT2139650.058.0844944
TCTCGTA2140100.058.067143
CGTATGC2153550.058.04736346
TCGTAGA2118800.058.02113735
CGTAGAA2123650.058.00177836
GTCACGT2134350.058.0009329
AGTCACG2141550.057.97841328
TGCCGTC2153650.057.96898350
Produced by FastQC (version 0.11.5)