FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25053282
Sequences flagged as poor quality0
Sequence length65
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGTCAATCTCGTATGCCGTCTTCTGCTTG256210.10226604242909172TruSeq Adapter, Index 18 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA37300.045.0315551
TATGCCG38800.042.87802548
CTCGTAT41050.040.59863344
CGTATGC41750.039.84728646
TCTCGTA42350.039.49169543
TCGTATG44500.037.78252845
ATCGGAA44600.037.76962
TGCCGTC44900.037.38350
ATGCCGT45550.036.7836849
GCCGTCT45950.036.33616651
TCGGAAG47650.035.5359273
ACACGTC48100.035.326913
CGTCTGA47700.035.3096916
ATCTCGT48100.034.83207742
AGTCACG48800.034.58001328
GTCACGT50200.033.5568629
TCACGTC51450.033.0835830
CCGTCTT53200.031.77248652
CGTCTTC53950.031.27611453
CGTCTGT54950.030.9201333
Produced by FastQC (version 0.11.5)