FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800838

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800838
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23616423
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC418930.1773892684764327No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC95300.031.6749554
GACCGCG96450.030.925975
CAGACCG98200.030.8296893
ACCGCGT98950.030.168946
TTCGACG27350.028.5814256
CCGCGTT108900.027.3849627
CGCGTTC111050.026.9079558
TCAGACC124750.025.6403962
GTTCGAC31050.025.180175
CGGTATA42600.023.33611320
TCGACGA34250.023.25377
CTCAGAC149500.022.8942811
CGACGAG35450.022.050558
GCGTTCT142700.021.0019459
CTACACG111200.019.7624357
AACGACG25050.019.31210316
AGTTCGA42550.019.001344
CGTTCTC172600.017.99605810
CGGTATG40800.017.71415921
ACACGTT124600.017.4240459
Produced by FastQC (version 0.11.5)