FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005804133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005804133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63461
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA1280220.173019649863697No Hit
CATAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA37985.984778052662265No Hit
CATAGAGGTGAGCGGCTGTCTCCATTCAACTTTCCTTGGCTCTATGTGTA37195.860292147933376No Hit
CATAGAGGTGAGCGGCTGTCTCCATGCTGAAGTCCCATGATCTATGTGTA16152.5448700776855078No Hit
CATAGATTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA15842.4960211783615134No Hit
CATAGATTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA15672.4692330722806135No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGAATTACAGGTGCCCGCCAC13612.1446242574179415No Hit
CATAGATTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT13442.1178361513370416No Hit
CATAGAGGTGAGCGGCTGTCTCCAAACTGGACAATGAGAGGATCTATGTG13322.098926899985818No Hit
CATAGAGGTGAGCGGCTGTCTCCAGATGATATGAACCCAAAGGTCTATGT10461.6482564094483225No Hit
CATAGATTGCATAGCCATGTGTAGCCATGCTGAAGTCCCATGATCTATGT10391.6372260128267753No Hit
CATAGAATCCTTTCCCCATTGCTTGCTCATTATTATTGGATGGGATCTAT9461.4906793148547928No Hit
CATAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT7851.2369801925592097No Hit
CATAGACCTCCTGTTTGACCATTTTGAACATAGCCTTTGGGGTTTCTATG7551.1897070641811507No Hit
CATAGAATAGCCAGCAGAAGGAAGCAGGAATTAGACTCTTGACATCTATG6210.9785537574258205No Hit
CATAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGAAAG6130.9659475898583382No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGGTCAGGAGATTGAGACCATCCTG5610.8840075006697026No Hit
CATAGAGGTGAGCGGCTGTCTCCAGTGTGTTTCTCTCTATCTGATTTCTT5570.8777044168859615No Hit
CATAGAGGTGAGCGGCTGTCTCCAGTGTATGCAAGACTAGGCAATTTACA5360.8446132270213201No Hit
CATAGAGGTGAGCGGCTGTCTCCACTAAAAATACAAAAATTAGCCAGGCA5160.8130978081026143No Hit
CATAGACTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA4700.7406123445895905No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT4390.6917634452655962No Hit
CATAGAAAGGCCTTCCTGTATCAGTCAGTGTCATCACTTATACGCCTCCC3500.5515198310773546No Hit
CATAGATGACTTGGGGGAAAATGGTAGAAACAGCCAGTTTTCCCTTCTAT3260.5137013283749073No Hit
CATAGAATCCTTTCCCCATTGCTTGTTCATTATTATTGGATGGGATCTAT3170.4995193898614897No Hit
CATAGACCATCCAGAGGTAATTCTGCTCATTATTATTGGATGGGATCTAT2770.4364885520240778No Hit
CATAGACTGTCATTGCTTGCCCATTGACTGTCACCCTGCTTATCAAACAT2770.4364885520240778No Hit
CATAGAAAGGCCTTCCTGTATCAGTCAGTGTGCCATGCTGAAGTCCCATG2740.43176123918627185No Hit
CATAGAGGTGAGCGGCTGTCTCCATCACTTATACGCCTCCCTGTCTATGT2740.43176123918627185No Hit
CATAGAAGTTCTGATCCACATTCACGTATAGCCCCTAAGCCCAGGTCTAT2320.3655788594569893No Hit
CATAGATGGAGAGAGTGAGGGAAAGGATTCTATGAGACCAACTCAGATGG2130.33563921148421866No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGTGCCAC1760.2773356864846126No Hit
CATAGACCAGACTTTTGAAAGAAGCACATGGAAGTACACAAAAAGGGCAT1740.274184144592742No Hit
CATAAATTCAACAGGCTTTCATCTATGTGTAGAACCATGTCGTCAGTGTA1700.26788106080900076No Hit
CATAGACTTCCTTGCCCCCATTTATTGCTGAAACATTTTTCTGCATGCAA1650.26000220607932434No Hit
CATAGAGGTGAGCGGCTGTCTCCACTAAAAATACAAAAATTAGCCATGCT1570.24739603851184191No Hit
CATAGAGCAGAAAACCTTTGAATAACAGATAGAGCAGGAATTAGACTCTT1520.23951718378216544No Hit
CATAGAGGTGAGCGGCTGTCTCCAGTGCGTTTCTCTCTATCTGATTTCTT1510.2379414128362301No Hit
CATAGATGAAAAGTCAAGCCAGCACAGGTTGGGAGAGCAGGGCAGGGCGG1470.23163832905248893No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTGCCAC1420.22375947432281243No Hit
CATAGAGGTGAGCGGCTGTCTCCACTAAAAATACAAAAAATTAGCCAGGC1370.21588061959313593No Hit
CATAGAGGTGAGCGGCTGTCTCCAGTGTGCTCATTATTATTGGATGGGAT1320.20800176486345945No Hit
CATAGACCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG1300.20485022297158884No Hit
CATAGACTGTCATTGCTTGCCCATTGCTTGTTTTTCTCAGGTTTGTCAAA1280.20169868107971825No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGACTACAGGTGCCTGCTAT1260.19854713918784767No Hit
CATAGAGGTGAGCGGCTGTCTCCACCAAAAATACAAAAATTAGCCAGGCA1210.19066828445817116No Hit
CATAGAGGTGAGCGGCTGTCTCCACACCCTGGTCCTCACTTGGGGTACTG1200.18909251351223585No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAAAAATACAAAAATTAGCCAGGCA1170.18436520067442996No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGTACCACAGGCATGTGCCAC1150.18121365878255938No Hit
CATAGAGCAGAAAACCTTTGAATAACAGAATAGAGCAGGAATTAGACTCT1140.17963788783662407No Hit
CATAGATTGCATAGCCATGTGTAGCCAGATGATATGAACCCAAAGGTCTA1130.17806211689068877No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTAATACAAAAAATTAG1060.16703172026914168No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGCACCAC1040.1638801783772711No Hit
CATAGAGGTGAGCGGCTGTCTCCAAGTCCGCGCAGAGCCTTCAGGTCAGA1020.16072863648540048No Hit
CATAGAAGCAGGATGAAAACATGGTACAGCTTCCACATGATGGACATCTA1000.1575770945935299No Hit
CATAGACTGTCATTGCTTGCCCATTGCTCATTATTATTGGATGGGATCTA1000.1575770945935299No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTGCCCGCCAG980.15442555270165928No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGACGACAGCCCGGCTACTG980.15442555270165928No Hit
CATAGATTGAGCTTAAATGATCTGTGCAAGGTTAAACAATATCCAAATCC960.1512740108097887No Hit
CATAGAATCCTTTCCCCATTGCTTGTTTTTGTCAAATTTGTTAAAAGAGG940.14812246891791808No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTGCCTGCCAC930.1465466979719828No Hit
CATAGAATCCTTTCCCCATTGCTTGTTTTTGTCAGGAAGTCAAATTGTCC890.1402436141882416No Hit
CATAGAATCCTTTCCCCATTGCTTGTTTTTGTCAGGTTTGTCAAAGATCA880.1386678432423063No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGAGATTATAGGTACTAATTTT840.1323647594585651No Hit
CATAGACATGCTAACATTTCTGGGGATATGCTAACATTACTGGGGACTGT830.1307889885126298No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCG820.12921321756669452No Hit
CATAGAGGTGAGCGGCTGTCTCCAGCTTCCACATGATGGACATCTATGTG820.12921321756669452No Hit
CATAGACCATCCAGAGGTAATTCTGCTACCGTACATCACTTATACGCCTC800.1260616756748239No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAAAAATACAAAAATTAGCCATGCT780.1229101337829533No Hit
CATAGAGGTGAGCGGCTGTCTCCATGATCTATGTGTAGAGCCATGCTGAA720.11345550810734151No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCCACCAC700.11030396621547092No Hit
CATAGAGGTGAGCGGCTGTCTCCACCAAAATACAAAAATTAGCCAGGCAT680.10715242432360034No Hit
CATAGATATGCCCATGGTTAGGAAGGCAGCTTCCACATGATGGACATCTA670.10557665337766502No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCGCCTGCCAC660.10400088243172972No Hit
CATAGATTGAGCTTAAATGATCTGTGCAAGACTAATGCAAGACTAGGCAA650.10242511148579442No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCCTCAGGTCA650.10242511148579442No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTCCAGGTCA640.10084934053985913No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)