FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005804140

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005804140
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178464
Sequences flagged as poor quality0
Sequence length150
%GC36

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGAGCTTGGGTGCGAACATCAAGGAAGTTTCTGTGAGATCTATGAGA173539.723529675452752No Hit
CATAGAGCTTTTTCTCCAGCCTCCAAGTAATAACCAGGCCCCTCTATGAG93975.265487717410795No Hit
CATAGACGGATTATTCTCTCCATGGTCTACCAGTTATTAGTTGAATGAGC82514.623341402187556No Hit
CATAGAGAAGACTCCCTCAACCCACCCACAGCCAGGAAAGAATCTATGAG73824.1364084633315406No Hit
CATAGATCACGTAGAAACTGGAGAGGCTAATCTGAAATCTTCGTGTTCTA67933.806369912139143No Hit
CATAGATCATGGGACTTCAGCATGGCTCAGGTATGTCTTTATTAGCTCTA57203.205128205128205No Hit
CATAGAAATGATGCTGAAGGGCCTGCCCTCTCTGCTGTTCCTTCTATGAG54823.0717679756141294No Hit
CATAGACTCATTGCACTTTCCACAACTGTGTGCTTGAACCAGATTCTATG46422.601084812623274No Hit
CATAGAGGAGGTGAAAGAAATCTACAATGGGTTCTGGGCCCCAGTGTGAA46192.5881970593509056No Hit
CATAGATGTTTTATCAAAGGCATGCTCATCAGGAACCATGGGAGGTCTAT40552.272166935628474No Hit
CATAGACCAGCAGACACTCCCATCATTTATTTTAAAGGACTGGAAGCCTC38762.171866594943518No Hit
CATAGACTCATTGCACTTTCCACAACTGTCATTGTCGCTGTCCCATCTAT37722.11359153666846No Hit
CATAGATGTTTTATCAAAGGCATGCTCATTTATTTTAAAGGACTGGAAGC36112.023377263761879No Hit
CATAGACGTGACTAAAAGCATGTTGGTGGAAGGGTAAGAGAGGGTCTATG30191.691657701273086No Hit
CATAGATGTTTTATCAAAGGCATGCTCATCAAGGAAGTTTCTGTGAGATC27331.5314012910166757No Hit
CATAGAAGTCACGAGTTTTGTTGTTGTTCTTCCTTTTCTGCCTTCATCTA23181.298861395015241No Hit
CATAGAAATGATGCTGAAGGGCCTGTTTCCCCACTCAAATACCATCTATG22471.2590774610005377No Hit
CATAGACTCATTGCACTTTCCACAACTGTCCCAACTGTCTCTGAAAAATT20681.1587771203155819No Hit
CATAGATCAAGGAAAACACCAGCCCACGCTGTCCTTCTCTGAATCTATGA20051.1234758830912677No Hit
CATAGAAATGATGCTGAAGGGCCTGCTGAAGTGATGGGCTAGATCTATGA18351.0282185762954994No Hit
CATAGACCAGCAGACACTCCCATCATCAGGAACCATGGGAGGTCTATGAG17750.9945983503675812No Hit
CATAGAGGAGGTGAAAGAAATCTACAATGGGCCCCAGTGTGAATACTTCT16350.916151156535772No Hit
CATAGAGAAGACTCCCTCAACCCACGCTGTCCTTCTCTGAATCTATGAGA15770.8836516048054509No Hit
CATAGAAGGACAAAGACATTTAGGACCCACGCTGTCCTTCTCTGAATCTA14230.7973596915904608No Hit
CATAGAAGCTGAGAAGACTTCCTAATGCAGAAGTAGGAAGTATTACCCCT13930.7805495786265017No Hit
CATAGAGCTTTTTCTCCAGCCTCCAAGGGGTAAGGCGTACTGGTCTATGA13240.7418863188093957No Hit
CATAGAAGCCATGAACACAGGATTGAGGAGCTGAGTCATGGGGTCTATGA13030.7301192397346243No Hit
CATAGAGTCTGAATGGTGGGAGAGTGAGGAGCTGAGTCATGGGGTCTATG11050.6191724941724942No Hit
CATAGACTCATTGCACTTTCCACAACTGTCTGCCTCTAAGTAAACTGAAT10670.597879684418146No Hit
CATAGAGTCCAACTATTCCAGCACCACGCTGTCCTTCTCTGAATCTATGA10120.5670611439842209No Hit
CATAGACACAACAGAGAGAACGGGCTACACTGTCATTGTCGCTGTCCCAT9900.5547337278106509No Hit
CATAGATGTGTAGCCTGGGTCTGACAAATGAGAAACCTGGATGCTTCTAT8830.4947776582391967No Hit
CATAGACACAACAGAGAGAACGGGCTACACTGACGCTATACCACACTACA8120.45499372422449347No Hit
CATAGAGTGCCTGTTTCCTCATTCGTACACTGCCCTCTCTGCTGTTCCTT8110.4544333871256948No Hit
CATAGACCAACATTTCTTCTCCCAGGGTACACTGACGCTATACCACACTA8110.4544333871256948No Hit
CATAGATCAAGGAAAACACCAGCCCAAGGGGTAAGGCGTACTGGTCTATG7910.44322664514972204No Hit
CATAGATGGCCAAGATACGAGCACATACACTGACGCTATACCACACTACA7900.44266630805092344No Hit
CATAGATGTGTAGCCTGGGTCTGACATGCCAGGAATCAATGTTCTATGAG7400.41464945311099155No Hit
CATAGAAGGACAAAGACATTTAGGACCCTGCAGTCTTTGGAGGAATCTAT7240.4056840595302134No Hit
CATAGAGTGCCTGTTTCCTCATTCGTATGCTGAAGTGATGGGCTAGATCT6940.38887394656625424No Hit
CATAGACCTCCGACAGTTTTTATGCACCTTCATTGTTGCTCTATGAGACC6890.38607226107226106No Hit
CATAGATGTGTAGCCTGGGTCTGACAGTGTGCTGAAGTGATGGGCTAGAT5810.32555585440200824No Hit
CATAGAGCTGTACTGCATCTTTTCTCAAACTGCTGGGGTTGCTCTATGAG5590.31322843822843827No Hit
CATAGATCAAGGAAAACACCAGCCCACACTGACGATCATTGTTCACTGCA5260.2947373139680832No Hit
CATAGACACAACAGAGAGAACGGGCTACACTGACGATCATTGTTCACTGC5130.2874529316837009No Hit
CATAGACCAGCAGACACTCCCATCATCAAGGAAGTTTCTGTGAGATCTAT5060.28353057199211046No Hit
CATAGACCTCCGACAGTTTTTATGCACTGTCCCAACTGTCTCTGAAAAAT4880.273444504213735No Hit
CATAGAGTCTGAATGGTGGGAGAGTGAAAGAAATCTACAAAGGATTCTGG4770.26728079612695No Hit
CATAGAAAAAACAGTTGGCCAGGCATCTATGCTGAAGTGATGGGCTAGAT4750.2661601219293527No Hit
CATAGAAAATTTGTGATGGTAAATCATGGGACATGCCAGGAATCAATGTT4740.2655997848305541No Hit
CATAGACCAACATTTCTTCTCCCAGGGTGGAAGGGTAAGAGAGGGTCTAT4550.25495337995337997No Hit
CATAGATGTTTTATCAAAGGCATGCTCATTGCTTCTTGTGGTAACTGGTC4250.23814326698942084No Hit
CATAGACGGATTATTCTCTCCATGGTCTGAGGAGAATTCAAGCTGCTCTA4190.23478124439662904No Hit
CATAGAGCTGTACTGCATCTTTTCTCAGACACACTGACGCTATACCACAC4100.22973821050744128No Hit
CATAGACCTCCGACAGTTTTTATGCACCCACAGCCAGGAAAGAATCTATG4040.22637618791464947No Hit
CATAGAAATGATGCTGAAGGGCCTGCAGAAGTAGGAAGTATTACCCCTCT3940.22077281692666306No Hit
CATAGATGTGTAGCCTGGGTCTGACAAGGGGTAAGGCGTACTGGTCTATG3910.21909180563026717No Hit
CATAGACCGCTTCCCAATTTTTGTAACAAGGGGTAAGGCGTACTGGTCTA3870.21685045723507262No Hit
CATAGATGAGGACACCATAAAGACAACACACTGACGATCATTGTTCACTG3730.2090057378518917No Hit
CATAGAGAAGACTCCCTCAACCCACCACACTGACGCTATACCACACTACA3640.20396270396270394No Hit
CATAGAAATGATGCTGAAGTGATGGGCTAGATCTATGAGACCAAGTCTCT3590.20116101846871076No Hit
CATAGAGTCCAACTATTCCAGCACCAATGGTGGAAGGGTAAGAGAGGGTC3510.1966783216783217No Hit
CATAGACCAGCAGACACTCCCATCATACACTGACGCTATACCACACTACA3410.1910749506903353No Hit
CATAGACGTGACTAAAAGCATGTTGGTACACTGACGACATGGTTCTACAA3310.18547157970234893No Hit
CATAGATGAGGACACCATAAAGACAACAACACTGACGCTATACCACACTA3070.17202348933118164No Hit
CATAGATGTGTAGCCTGGGTCTGACAGTGTCAAGGGGTAAGGCGTACTGG3030.1697821409359871No Hit
CATAGATGAGGACACCATAAAGACAACAAGGGGTAAGGCGTACTGGTCTA3010.1686614667383898No Hit
CATAGATGTGTAGCCTGGGTCTGACAGTGTAGGCTAATCTGAAATCTTCG2990.16754079254079254No Hit
CATAGAGTAGTTAAAACCCACAATGACAGTACACTGCCCTCTCTGCTGTT2920.16361843284920208No Hit
CATAGACGTGACTAAAAGCATGTTGGTGCATTACAGAGAGGAGATCTATG2910.16305809575040345No Hit
CATAGACCAGCAGACACTCCCATCATTCTGCTGAAGTGATGGGCTAGATC2850.1596960731576116No Hit
CATAGATGGCCAAGATACGAGCACATACACTGACGATCATTGTTCACTGC2850.1596960731576116No Hit
CATAGACGGATTATTCTCTCCATGGTCTACAAAGGATTCTGGGCCATCTA2800.15689438766361843No Hit
CATAGACCAACATTTCTTCTCCCAGGGTACACTGGCTCAGGTATGTCTTT2790.15633405056481978No Hit
CATAGAAAAAACAGTTGGCCAGGCATGGTGGAAGGGTAAGAGAGGGTCTA2750.15409270216962526No Hit
CATAGACGGATTATTCTCTCCATGGTCTACACTGACGACATGGAGACAGC2700.15129101667563208No Hit
CATAGAAGGACAAAGACATTTAGGACCCGATGTTCTTCCTTTTCTGCCTT2630.1473686569840416No Hit
CATAGATCATGGGACTTCAGCATGGAGACAGCCGCTCACCTCTATGAGAC2620.14680831988524295No Hit
CATAGAAAGGTGCAATCAAGCCAGTTACACTGACGCTATACCACACTACA2590.14512730858884704No Hit
AAGGATTCTGGGCCATCTATGAGACCAAGTCTCTGCTACCGTAAATCGAG2550.1428859601936525No Hit
CATAGATCAAGGAAAACACCAGCCCACCCACAGCCAGGAAAGAATCTATG2520.1412049488972566No Hit
CATAGATTGTGTGTCTCTTCTATATTAACAGTCACACTGGCTCAGGTATG2470.1384032634032634No Hit
CATAGAAGTCACGAGTTTTGTTGTTGTTACACTGACGCTATACCACACTA2450.13728258920566613No Hit
CATAGAGTCTGAATGGTGGGAGAGTGAATGGTGGAAGGGTAAGAGAGGGT2450.13728258920566613No Hit
CATAGACACAACAGAGAGAACGGGCTAGATGCTGAAGTGATGGGCTAGAT2440.1367222521068675No Hit
CATAGACGGATTATTCTCTCCATGGTCTAAATATGCTGAAGTGATGGGCT2420.13560157790927022No Hit
CATAGAAGCCATGAACACAGGATTGATACGGTAGCAGCATAATTTCCCAT2400.13448090371167293No Hit
CATAGACCTAAAAGGCTATTGAAAGGAAGGCTAATCTGAAATCTTCGTGT2390.1339205666128743No Hit
CATAGACCAACATTTCTTCTCCCAGGGTATGATGCTGAAGTGATGGGCTA2360.1322395553164784No Hit
CATAGAAATGATGCTGAAGGGCCTGCTGCTGAAGTGATGGGCTAGATCTA2280.12775685852608928No Hit
CATAGACAGTGCCTTTGTGCGTGTTAGGAGAGCTTCACAACCTTGAAAGG2260.12663618432849202No Hit
CATAGACCTCCGACAGTTTTTATGCACCTTCCCCACACCCTCTTCTTCTA2260.12663618432849202No Hit
CATAGAAGCCATGAACACAGGATTGATAGGCTTAGAGGGCTTGCTCTATG2200.1232741617357002No Hit
CATAGAAGTCACGAGTTTTGTTGTTGTTACACTGGCTCAGGTATGTCTTT2140.11991213914290837No Hit
CATAGAGTGCCTGTTTCCTCATTCGTCAGTGTTGATAGGCTTAGAGGGCT2130.11935180204410974No Hit
CATAGAGTCTGAATGGTGGGAGAGTGCTGAAGTGATGGGCTAGATCTATG1950.10926573426573427No Hit
CATAGAGCTGTACTGCATCTTTTCTCAGACTGCTGGGGTTGCTCTATGAG1930.108145060068137No Hit
CATAGACCTCCGACAGTTTTTATGCACACACTGACGCTATACCACACTAC1910.10702438587053972No Hit
CATAGAAATGATGCTGAAGGGCCTGCACATAGAAATGATGCTGAAGTGAT1900.1064640487717411No Hit
CATAGATGTTTTATCAAAGGCATGCTCATCATAGTTGTCTGCCTCTAAGT1870.10478303747534516No Hit
CATAGATTGTGTGTCTCTTCTATATTAACAGTCTGTTTCCCCACTCAAAT1850.10366236327774789No Hit
CATAGAGAAGACTCCCTCAACCCACCATTAGCAGCATAATTTCCCATGTC1850.10366236327774789No Hit
CATAGAGAAGACTCCCTCAACCCACCATAACACTGACGCTATACCACACT1840.10310202617894926No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)