FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005804161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005804161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86416
Sequences flagged as poor quality0
Sequence length150
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA3812044.112201444177No Hit
CATAGATTGCACATAAAGTCTGATCCCATCTCAAGTCTCACTCTTCTATG39624.58479911127569No Hit
CATAGACTACCACTACCCAGCACAGCTACTTCTCTATGCACTTTGCCACA22942.654600999814849No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTGTGATTACAGGTG18022.085261988520644No Hit
CATAGACCATCCTCCCACCTTGACCTCCTGAGTAGCTGAGACCACAGGCA16261.8815960007406036No Hit
CATAGACTACCACTACCCAGCACAGGAGCACCCTCTATGTGTAGAACCAT15521.7959637104239954No Hit
CATAGAAGCAGCATTGATCATCCTCCACCCCATCCTACATGTGTCTATGT13561.569153860396223No Hit
CATAGAGATGTCAAGAGCAATGCCCAGCCACCCCATCCTACATGTGTCTA8200.948898352157008No Hit
CATAGACTACCACTACCCAGTACAGGAGCACCCTCTATGTGTAGAACCAT7730.894510275874838No Hit
CATAGACTACCACCACCCAGTACAGGAGCACCCTCTATGTGTAGAACCAT6040.6989446398815035No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTGGGATTACAGGTG6010.6954730605443437No Hit
CATAGAGCCTGGCATTTGAGGTAAGGCTTGTGAGGGCTGAAGTCTATGTG5520.6387705980374004No Hit
CATAGACTCAAGAAACTTACAATCATGGCAGAAGGTGTAAGGGAAGCCAC5410.6260414738011479No Hit
CATAGACCATCCTCCCACCTTGACCTCTTAAGTAGCTGGGACTACAGGTG4810.5566098870579522No Hit
CATAGACTGTTCTTTTAAATTAACACAGTCAGAGATGGGTTTTTGCCAGA4030.4663488242917978No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTATTACTACAGGTG4000.462877244954638No Hit
CATAGAGATGTCAAGAGCAATGCCCACCCCATCCTACATGTGTCTATGTG3770.43626180336974635No Hit
CATAGACCATCCTCCCACCTTGACCTTCCAAAGTGCTAGGATAATAGGTG3510.40617478244769484No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAGAGTGCTGGAATTATAGGCG3340.38650249953712273No Hit
CATAGACCATCCTCCCACCTTGACCTCCATCTTGACCTCCCAAAGTGCTG3330.38534530642473613No Hit
CATAGACCATCCTCCCACCTTGACCTCCAGAGTAGCAGGGACTACAGGCA3180.36798740973893723No Hit
CATAGACTCAAGAAACTTACAATCATGGCAGAAAGTGAAGGAGAGGCCAC3120.3610442510646177No Hit
CATAGACTCAAGAAACTTACAATCATGGCATACGGTAGCAGAGGCACTTG3020.34947231994075173No Hit
CATAGAGATGTCAAGAGCAATGCCCATGCTGAAGTCCCATGATCTATGTG2850.32980003703017957No Hit
CATAGACCATCCTCCCACCTTGACCTCCCGAGTAGCTGGGACTACAGGTG2750.3182281059063137No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTGGGATTACAGGCA2380.2754119607480096No Hit
CATAGAGGTGAGCGGCTGTCTCCAAGTCCGCGCAGAGCCTTCAGGTCAGA2350.27194038141084986No Hit
CATAGAAGGTGATAATCATGACAGCCACCCCATCCTACATGTGTCTATGT2350.27194038141084986No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCG2230.2580540640622107No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTGGGATTACAGGCG2220.2568968709498241No Hit
CATAGATCCCAGGCTTTAGAAAATATACCACCCAGTACAGGAGCACCCTC2130.24648213293834476No Hit
CATAGAGGTGAGCGGCTGTCTCCACGAGTCCGCGCAGAGCCTTCAGGTCA2120.24532493982595813No Hit
CATAGAACATCGCACACACATGGAACCATGTCGTCAGTGTGCTTCCCTGC2050.237224588039252No Hit
CATAGACTCAAGAAACTTACAATCATGGCAGAAGGCCATGGGGAGCCAGC2000.231438622477319No Hit
CATAGACCATCCTCCCACCTTGACCTATCCTCCCATCTCAAGTCTCACTC1970.2279670431401592No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCGGGTCA1920.22218107757822625No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAACAGGTACTGGGATTACAGG1880.21755230512867985No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTGGGATTACAGGAG1830.2117663395667469No Hit
CATAGAGGTGAGCGGCTGTCTCCACAGGTCCGCGCAGAGCCTTCAGGTCA1810.20945195334197372No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGGGCTGGGATGACACGCA1770.20482318089242732No Hit
CATAGACCATCTGCGCATCCTGTCTGAGAAGTGAGGAGCCCCTCTGCCCG1710.19788002221810774No Hit
CATAGAGCCTGGCATTTGAGGTAAGGCACTTGGTCCAGACTTTCTATGTG1650.1909368635437882No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCGGAGCCTTCAGGTCA1620.1874652842066284No Hit
CATAGAGATGTCAAGAGCAATGCCCGGCCACATTATTTTCTATGTGTAGA1590.18399370486946862No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGCCA1550.17936493241992224No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGGGCCTTCAGGTCA1500.17357896685798926No Hit
CATAGAAGGTGATAATCATGACAGCCAACTGATCTTTGACAAAGCAAACA1490.17242177374560266No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCCTCAGGTCA1470.17010738752082946No Hit
CATAGAGGTGAGCGGCTGTCTCCACACGTCCGCGCAGAGCCTTCAGGTCA1450.1677930012960563No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGTTAGGATTACAGGCA1420.1643214219588965No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAGTTGCTGGGACCACAGGTG1400.1620070357341233No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTCCAGGTCA1370.15853545639696354No Hit
CATAGAGATGTCAAGAGCAATGCCCAAGCTCACTTTTCTCCAGAGAAACT1310.15159229772264396No Hit
CATAGACTCAAGAAACTTACAATCATGGCAGAAAAGGAAGCAAGCATATC1310.15159229772264396No Hit
CATAGAGTTTTGTTCCCTTGCTGGCAGTGTAGGGATAGTTTGAGGGTAAT1300.15043510461025736No Hit
CATAGAGATGTCAAGAGCAATGCCCAGCCACATTATTTTCTATGTGTAGA1290.14927791149787076No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTTAGGTCA1260.14580633216071098No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAGTAACTGGGACTACAGGTG1260.14580633216071098No Hit
CATAGACTCAAGAAACTTACAATCATGGCAAAAGGCAAAGGAGAAGCAGG1250.14464913904832438No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAGTAACTGGGACTACAGTCA1240.14349194593593778No Hit
CATAGAGATGTCAAGAGCAATGCCCATGCCTAGCCATTTTTGGTTCTATG1240.14349194593593778No Hit
CATAGACTCAAGAAACTTACAATCATGGCAGGAGGGGAAGCAAACATGTC1200.1388631734863914No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTGAGATTACACACG1170.13539159414923163No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTAGGATTACAAGTG1140.13192001481207183No Hit
CATAGAGGTGAGCGGCTGTCTCCATGCTGAAGTCCCATGATCTATGTGTA1140.13192001481207183No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGCCCGCGCAGAGCCTTCAGGTCA1130.13076282169968526No Hit
CATAGACTCAAGAAACTTACAATCATGGCAGAAGGCAAAAAAGAAGCAGG1120.12960562858729863No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTTA1090.12613404925013885No Hit
CATAGAAGCAGCATTGATCATCCTCCACCCAGTACAGGAGCACCCTCTAT1060.12266246991297906No Hit
CATAGACCATCCTCCCACCTTGACCTCATCCTCCCATCTCAAGTCTCACT1060.12266246991297906No Hit
CATAGACCATCCTCCCACCTTGACCTTCCAAAGTGCTGGGATTACAGGTG1050.12150527680059248No Hit
CATAGATCCCAGGCTTTAGAAAATATACCACCACCCAGTACAGGAGCACC1030.11919089057581929No Hit
CATAGAGAATACAATGAACTGTGATGGCATCAACATAGGCACACAGACCA1020.1180336974634327No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTTGGATTACAGGTG960.11109053878911312No Hit
CATAGACATGAGTGGCCGAACAGACCACTCCTATGTAATGTCTTATTTTG920.10646176633956675No Hit
CATAGACCATCCTCCCACCTTGACCTTCCCGGTAGCTGGGACTACAGGCA880.10183299389002036No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCACAGAGCCTTCAGGTCA870.10067580077763377No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)