FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005804178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005804178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145452
Sequences flagged as poor quality0
Sequence length150
%GC37

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGAGCTTGGGTGCGAACATCAAGGAAGTTTCTGTGAGATCTATGAGA1675911.522014135247368No Hit
CATAGAGCTTTTTCTCCAGCCTCCAAGTAATAACCAGGCCCCTCTATGAG85525.879602893050628No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCTGCG74645.131589802821549No Hit
CATAGAGAAGACTCCCTCAACCCACCCACAGCCAGGAAAGAATCTATGAG69364.768583450210379No Hit
CATAGACGGATTATTCTCTCCATGGTCTACCAGTTATTAGTTGAATGAGC69004.743833017077799No Hit
CATAGAGGAGGTGAAAGAAATCTACAATGGGTTCTGGGCCCCAGTGTGAA58584.027445480295905No Hit
CATAGAAATGATGCTGAAGGGCCTGCCCTCTCTGCTGTTCCTTCTATGAG49903.430685036988147No Hit
CATAGACTCATTGCACTTTCCACAACTGTGTGCTTGAACCAGATTCTATG37222.558919781096169No Hit
CATAGATGTTTTATCAAAGGCATGCTCATTTATTTTAAAGGACTGGAAGC35762.4585430245029287No Hit
CATAGAAATGATGCTGAAGGGCCTGTTTCCCCACTCAAATACCATCTATG34652.3822291890108076No Hit
CATAGATCATGGGACTTCAGCATGGCTCAGGTATGTCTTTATTAGCTCTA33862.3279157385254243No Hit
CATAGACTCATTGCACTTTCCACAACTGTCATTGTCGCTGTCCCATCTAT32742.2509143910018423No Hit
CATAGATGTTTTATCAAAGGCATGCTCATCAGGAACCATGGGAGGTCTAT29922.0570359981299675No Hit
CATAGACTCATTGCACTTTCCACAACTGTCCCAACTGTCTCTGAAAAATT27051.8597200451007891No Hit
CATAGAAGTCACGAGTTTTGTTGTTGTTCTTCCTTTTCTGCCTTCATCTA26941.8521574127547231No Hit
CATAGATCACGTAGAAACTGGAGAGGCTAATCTGAAATCTTCGTGTTCTA24991.7180925666199158No Hit
CATAGACCAGCAGACACTCCCATCATTTATTTTAAAGGACTGGAAGCCTC20291.394961911833457No Hit
CATAGAAATGATGCTGAAGGGCCTGCTGAAGTGATGGGCTAGATCTATGA14350.98657976514589No Hit
CATAGATGTGTAGCCTGGGTCTGACATGCCAGGAATCAATGTTCTATGAG12900.8868905205841102No Hit
CATAGAGCTTTTTCTCCAGCCTCCAAGGGGTAAGGCGTACTGGTCTATGA12630.8683276957346754No Hit
CATAGACTCATTGCACTTTCCACAACTGTCTGCCTCTAAGTAAACTGAAT11790.8105766850919891No Hit
CATAGAGGAGGTGAAAGAAATCTACAATGGGCCCCAGTGTGAATACTTCT11280.7755135714875011No Hit
CATAGAAGCCATGAACACAGGATTGAGGAGCTGAGTCATGGGGTCTATGA10930.7514506503863818No Hit
CATAGAGTCTGAATGGTGGGAGAGTGAAAGAAATCTACAAAGGATTCTGG10840.7452630421032368No Hit
CATAGACCAGCAGACACTCCCATCATCAGGAACCATGGGAGGTCTATGAG10530.7239501691279597No Hit
CATAGAGAAGACTCCCTCAACCCACGCTGTCCTTCTCTGAATCTATGAGA9910.6813244231774056No Hit
CATAGATGTTTTATCAAAGGCATGCTCATCAAGGAAGTTTCTGTGAGATC9300.639386189258312No Hit
CATAGACCAACATTTCTTCTCCCAGGGTACACTGACGCTATACCACACTA8760.6022605395594423No Hit
CATAGACCAACATTTCTTCTCCCAGGGTCTACCAGTTATTAGTTGAATGA8640.5940103951819157No Hit
CATAGACAGTGCCTTTGTGCGTGTTACCGTACAAGGGGTAAGGCGTACTG8370.5754475703324808No Hit
CATAGACCTCCGACAGTTTTTATGCACCTTCATTGTTGCTCTATGAGACC8230.5658224018920331No Hit
CATAGAAGGACAAAGACATTTAGGACCCTGCAGTCTTTGGAGGAATCTAT7730.5314468003190056No Hit
CATAGATCAAGGAAAACACCAGCCCACGCTGTCCTTCTCTGAATCTATGA7060.48538349421114874No Hit
CATAGATCAAGGAAAACACCAGCCCACCCACAGCCAGGAAAGAATCTATG6760.4647581332673322No Hit
CATAGAGCTGTACTGCATCTTTTCTCAAACTGCTGGGGTTGCTCTATGAG6430.44207023622913405No Hit
CATAGAAGCTGAGAAGACTTCCTAATGCAGAAGTAGGAAGTATTACCCCT6210.4269449715370019No Hit
CATAGACATTTAATCCTCACAATGACCCATACACTGACGATCATTGTTCA5710.3925693699639744No Hit
CATAGACTTGAAAGTGATAACTGAGAGGTAAACACACTGACGCTATACCA4680.3217556307235377No Hit
CATAGAGTGCCTGTTTCCTCATTCGTGTCTGTTTCCCCACTCAAATACCA4550.3128179743145505No Hit
CATAGAGTCTGAATGGTGGGAGAGTGCTGAAGTGATGGGCTAGATCTATG4510.3100679261887083No Hit
CATAGACCTCCGACAGTTTTTATGCACTGTCCCAACTGTCTCTGAAAAAT4500.30938041415724776No Hit
CATAGAGAAGACTCCCTCAACCCACCACGCTGTCCTTCTCTGAATCTATG4300.2956301735280368No Hit
CATAGATTGTGTGTCTCTTCTATATTAACAGTCCACTGACGCTATACCAC3910.2688172043010753No Hit
CATAGAGTGCCTGTTTCCTCATTCGTACATAGAGTGTCTGTTTCCCCACT3680.2530044275774826No Hit
CATAGACCAACATTTCTTCTCCCAGGGTACACTGACGACATGGCTCAGGT3300.22687897038198168No Hit
CATAGATTGTGTGTCTCTTCTATATTAACAGTCCACGCTGTCCTTCTCTG3200.22000385006737616No Hit
CATAGAAATGATGCTGAAGGGCCTGCAGAAGTAGGAAGTATTACCCCTCT3070.21106619365838902No Hit
CATAGACGTGACTAAAAGCATGTTGGTACACTGACGACATGGTTCTACAA3030.20831614553254685No Hit
CATAGAAATGATGCTGAAGGGCCTGCGATGCTGAAGTGATGGGCTAGATC2840.19525341693479636No Hit
CATAGAGCTGTACTGCATCTTTTCTCAGACTGCTGGGGTTGCTCTATGAG2710.1863157605258092No Hit
CATAGACGTGACTAAAAGCATGTTGGTGCATTACAGAGAGGAGATCTATG2600.17875312817974315No Hit
CATAGAAAATTTGTGATGGTAAATCATGGGAGGTCATCAGGAACCATGGG2540.17462805599097986No Hit
CATAGAAATGATGCTGAAGGGTCTGTTTCCCCACTCAAATACCATCTATG2510.17256551989659819No Hit
CATAGAAATGATGCTGAAGGGCCTGCTGCTGAAGTGATGGGCTAGATCTA2480.17050298380221654No Hit
CATAGAAATGATGCTGAAGTGATGGGCTAGATCTATGAGACCAAGTCTCT2430.16706542364491378No Hit
CATAGAAATGATGCTGAAGGGCCTGCACTGACGACATGGTTCTACACATA2370.1629403514561505No Hit
CATAGAGTCTGAATGGTGGGAGAGTGATAGGCTTAGAGGGCTTGCTCTAT2310.1588152792673872No Hit
CATAGAGAAGACTCCCTCAACCCACCACACTGACGATCATTGTTCACTGC2240.15400269504716332No Hit
CATAGACGTGACTAAAAGCATGTTGGTGTTTGCAGATTTGGACCTGCGAG2230.15331518301570277No Hit
CATAGAAATGATGCTGAAGGGCCTGCAGTCTTTGGAGGAATCTATGAGAC2210.15194015895278168No Hit
CATAGACCGCTTCCCAATTTTTGTAACAACACCCACAGCCAGGAAAGAAT2190.15056513488986056No Hit
CATAGACACAACAGAGAGAACGGGCTACACTGACGATCATTGTTCACTGC2090.14369001457525507No Hit
CATAGAAATGATGCTGAAGGGCCTGTGCTGAAGTGATGGGCTAGATCTAT2070.14231499051233396No Hit
CATAGAAAATTTGTGATGGTAAATCATGGGAGGTCTCATCAGGAACCATG2030.13956494238649175No Hit
CATAGAGTAGTTAAAACCCACAATGACAGTGTACAAATGAGAAACCTGGA1980.136127382229189No Hit
CATAGAGTAGTTAAAACCCACAATGACAGTTTTTGTGCACCTTCATTGTT1980.136127382229189No Hit
CATAGAGTGCCTGTTTCCTCATTCGTTCTACACATAGAGTGTCTGTTTCC1890.12993977394604406No Hit
CATAGACCCAGATGCTGTCCCACTAAGACCCACGCTGTCCTTCTCTGAAT1890.12993977394604406No Hit
CATAGAAATGATGCTGAAGGGCCTGGTTCTACACATAGAAATGATGCTGA1790.12306465363143854No Hit
CATAGATTGTGTGTCTCTTCTATATTAACAGTCTACACTGCCCTCTCTGC1750.12031460550559635No Hit
CATAGACCAGCAGACACTCCCATCATACACTGCCCTCTCTGCTGTTCCTT1750.12031460550559635No Hit
CATAGACCTCCGACAGTTTTTGTGCACCTTCATTGTTGCTCTATGAGACC1700.11687704534829359No Hit
CATAGACACAACAGAGAGAACGGGCTACACTGACGCTATACCACACTACA1690.11618953331683304No Hit
CATAGAAAAAACAGTTGGCCAGGCATTCAAGACCAGCTTGACCAACATGG1690.11618953331683304No Hit
CATAGACTCATTGCACTTTCCACAACTGCCCTCTCTGCTGTTCCTTCTAT1590.10931441300222754No Hit
CATAGAAAATTTGTGATGGTAAATCATGGGAGGTCTATGAGACCAAGCCT1570.10793938893930644No Hit
AAGGATTCTGGGCCATCTATGAGACCAAGTCTCTGCTACCGTAATGCCTA1540.10587685284492478No Hit
CATAGAGAAGACTCCCTCAACCCACCTTCCCCACACCCTCTTCTTCTATG1510.10381431675054315No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)