FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005804200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005804200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123664
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCTGCG4638937.51212964160952No Hit
CATAGAGGGCTGTTTTGAGTGTTTGCCGTGGAGGAAAGGAGGTCTATGAG56334.555084745762712No Hit
CATAGAGCTTCTGATCACTTTCGCCCCACCCTTATGATCGTATCTATGAG40063.239422952516496No Hit
CATAGAACCAGAGTAATGAGGGATGCTCAGGTCTCCAATGGTGTCTATGA32002.58765687669815No Hit
CATAGACTCAAGCAATCCTCCCACCTCAGCCTCCCAAGTAGCCAGGACTA29532.3879221115280114No Hit
CATAGACTCAAGCAATCCTCCCACCTCAGCCTCCCAAATAGCCAGGACTA27722.241557769439772No Hit
CATAGAACTTGTAATCATTCCCTACTTCCCACACTCATGCAAAGCTGGTC26442.1380514943718465No Hit
CATAGAACCAGAGTAATGAGGGATGCTCTCTCCAGAACCCACCATCTATG22431.8137857420106092No Hit
CATAGATGTGGCTATGCAGACTGAGCAGAGTTGGGTGTGTCTATGAGACC19921.6108164057445984No Hit
CATAGATCATGCAAAGAACACAGGCCACACTCATGCAAAGCTGGTCTATG17071.3803532151636693No Hit
CATAGAAGATCCCATAGCAGCTTTGCTACCGTAAGTTTTTCCAAATATAA12691.02616768016561No Hit
CATAGAACTTTGTGCCCCCAGTGTCTCCCCTCTATGAGACCAAGTCTCTG12250.9905873981110105No Hit
CATAGAGGGCTGTTTTGAGTGTTTGCAGATTTGGACCTGCGAGCGGGTTC11330.9161922629059387No Hit
CATAGAACTTTGTGCCCCCAGTGTCTTCTTATAGCCAGTGTCTCCCCTCT10770.8709082675637211No Hit
CATAGAACTTTGTGCCCCCAGTGTCTTAGGTAGAGGAGTTTCAAACATCT9520.7698279208176996No Hit
CATAGATCATGCAAAGAACACAGGCAGATCATGAGGTCAGGTCTATGAGA8120.6566179324621555No Hit
CATAGATGTGGCTATGCAGACTGAGTAGAGTTGGGTGTGTCTATGAGACC7450.602438866606288No Hit
CATAGAGGGCTGTTTTGAGTGTTTGCTACCGTAGACGGAGTGAGGGCAGT5670.45850045283995344No Hit
CATAGAGGGCTGTTTTGAGTGTTTGCCGTGGAGGAAAGCAATCACCGCCA5590.45203131064820806No Hit
CATAGAGCCTGGGGTAGTTTTCTCTCATTTCCCCTCTCTCTGAATAACTC5190.41968559968948116No Hit
CATAGAGGGCTGTTTTGAGTGTTTGCCGTGGAGGGAAGGAAGGGCAAACT4940.3994695303402769No Hit
CATAGACCCTTCTCCATGCCACATCATCCCATGGGTCCTTTTCACATCAT4410.3566114633199638No Hit
CATAGAAGGAGGGGAAACTGAGGCCAAGTGGAGGGAAGGAAGGGCAAACT3970.32103118126536423No Hit
CATAGACTCAAGCAATCCTCCCACCTCAGCTTCCCTAAGTACCAGGATTA3930.31779661016949157No Hit
CATAGATGTGGCTATGCAGACGGAGTGAGGGCAGTAAGTCTATGAGACCA3920.3169879673955234No Hit
CATAGAAGGAGGGGAAACTGAGGCCAGGCGCGGTGGCTCATGCCTGTAAT3900.315370681847587No Hit
CATAGAGGGCTGTTTTGAGTGTTTGCCATGTCTTCTGTGTTTGGTCTATG3830.3097101824298098No Hit
CATAGAACTTTGTGCCCCCAGTGTCTCCCCTCTATGAGACCAAGTCTTCT3740.3024323974640963No Hit
CATAGAACTTTGTGCCCCCAGTGTCTCCCCTTCTTATAGCCAGTGTCTCC3390.27412990037521023No Hit
CATAGATGTGCCTCTCCAACATTCATGCAAAGCTGGTCTATGAGACCAAG3360.2717039720533057No Hit
CATAGAAGGAGGGGAAACTGAGGCCAAGTCTCTGCGGAGGGAAGGAAGGG3220.26038297321775133No Hit
CATAGAACTTTGTGCCCCCAGTGTCTCCCCTCTATTCTTATAGCCAGTGT2920.23612368999870614No Hit
CATAGAACACAGATCTGCTTGGAGGGAAGGAAGGGCAAACTCTATGAGAC2860.23127183335489715No Hit
CATAGAAGAATTGCTTGAATCCGGGAGGCAGAGGTTGCAGTGAGCTGAGA2840.2296545478069608No Hit
CATAGATGTGGCTATGCAGACTGAGTGAGGGCAGTAAGTCTATGAGACCA2840.2296545478069608No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCCGCG2840.2296545478069608No Hit
CATAGAACTTTGTGCCCCCAGTGTCTCCCTTCTTATAGCCAGTGTCTCCC2790.22561133393711993No Hit
CATAGACACCTGGATCCTGTGTTGTCTCATTTCCCCTCTCTCTGAATAAC2660.2150989778755337No Hit
CATAGAACTTTGTGCCCCCAGTGTCTCCCCTCTATGAGACCAAGTTCTTA2640.21348169232759737No Hit
CATAGAAGGAGGGGAAACTGAGGCCAAGTCTCTGCTACCGTAGCATGTTG2620.211864406779661No Hit
CATAGACCCTTCTCCATGCCACACTCATGCAAAGCTGGTCTATGAGACCA2590.2094384784577565No Hit
CATAGAGCCTGGGGTAGTTTTCTCTCATCTATGAGACCAAGTCTCTGCCA2520.2037779790399793No Hit
CATAGAAGATGGTCTGCTACACTGGTCCCCAGGCTCTGACCTCTGAACCC2410.19488290852632942No Hit
CATAGAAGGAGGGGAAACTGAGGCCAAGTCAGCATGTTGAGATGAATAAT2410.19488290852632942No Hit
CATAGAAGGAGGGGAAACTGAGACCAAGTCTCTGCTACCGTAAAGTGCGA2400.19407426575236125No Hit
CATAGAGCTTCTGATCACTTTCGCCCTCTCTCCAGAACCCACCATCTATG2320.18760512356061587No Hit
CATAGAAGATGGTCTGCTACACTGGTGTTCCACACTCATGCAAAGCTGGT2310.1867964807866477No Hit
CATAGAAGGAGGGGAAACTGAGGCCAAGCCATGGGTCCTTTTCACATCAT2300.1859878380126795No Hit
CATAGAAGGAGGGGAAACTGAGGCCAAGTCTATTCACCAAGTCACCCAGC2220.17951869582093413No Hit
CATAGAAGGAGGGGAAACTGAGGCCAAGTCAGTGCAGCTATATTGGAGGA2180.17628412472506144No Hit
CATAGAGCTTCTGATCACTTTCGCCCATGGGTCCTTTTCACATCATCTAT2100.1698149825333161No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCGGATTTGGACCTGCG2090.1690063397593479No Hit
CATAGAAGATGGTCTGCTACACTGGTCTACACATAGAAGATGGTCTGCCA2050.16577176866347523No Hit
CATAGAAGGAGGGGAAACTGAGGCCAAGTCTCTGCTACCGTAGGAAAGCA2020.1633458403415707No Hit
CATAGAAGGAGGGGAAACTGAGGCCAAGGAGGGAAGGAAGGGCAAACTCT2020.1633458403415707No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTCGCAGATTTGGACCTGCG1980.16011126924569802No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTCTGCAGATTTGGACCTGCG1980.16011126924569802No Hit
CATAGAGGTCGCAGCTACCCAGAAAGCCACCTGGGAGCGCCAGGACCTGC1970.15930262647172985No Hit
CATAGAAGAATTGCTTGAATCCGGGAGGGAAGGAAGGGCAAACTCTATGA1890.15283348427998447No Hit
CATAGAACTTTGTGCCCCCAGTGTCTCTTCTTATAGCCAGTGTCTCCCCT1850.1495989131841118No Hit
CATAGAAGGAGGGGAAACTGAGGCCAGGAGGGAAGGAAGGGCAAACTCTA1800.14555569931427093No Hit
CATAGACCCTTCTCCATGCCACATCACTGACGACATGGTTCTACACATAG1770.1431297709923664No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTCGGACCTGCG1710.13827791434855738No Hit
CATAGAAGATGGTCTGCTACACTGGTACACTGACGCCATGGGTCCTTTTC1670.1350433432526847No Hit
CATAGAAGGAGGGGAAACTGAGGCCACACTCATGCAAAGCTGGTCTATGA1650.13342605770474836No Hit
CATAGAAGATGGTCTGCTACACTGGTACACTGACGACATGGTTCTACACA1560.1261482727390348No Hit
CATAGAAGGAGGGGAAACTGAGGCCAAATGGCATTAAATGACTTGCCCAA1550.12533962996506665No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGCTTGCAGATTTGGACCTGCG1530.12372234441713027No Hit
CATAGAACTTTGTGCCCCCAGTGTCTCCCCTCTTCTTATAGCCAGTGTCT1490.1204877733212576No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGGTTTGGACCTGCG1490.1204877733212576No Hit
CATAGAAGATGGTCTGCTACACTGGTGCATGAGCATGTGACCAAACAGAA1460.11806184499935307No Hit
CATAGATCATGGGACTTCAGCATGGCATGTTGAGATGAATAATTGAGACT1380.11159270280760772No Hit
CATAGAAGGAGGGGAAACTGAGGCCAGGAGCGATGGTTTATGCCTGTAAT1330.10754948893776685No Hit
CATAGATGTGGCTATGCAGACTGAGTGGGGGCAGATTAACAGGTCATCTA1310.1059322033898305No Hit
CATAGAACTTGTAATCATTCCCTACTTCCCTGCTGCCAAACCATTTGGGA1300.10512356061586232No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGGCCTGCG1290.10431491784189417No Hit
CATAGATCATGCAAAGAACACAGGCCCCACCCTTATGATCGTATCTATGA1270.10269763229395783No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGGTTGCAGATTTGGACCTGCG1260.10188898951998965No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)