FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005804229

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005804229
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99805
Sequences flagged as poor quality0
Sequence length150
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNAGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA60406.051801011973348No Hit
NNTAGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA60106.021742397675467No Hit
NATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA58935.904513801913732No Hit
NNNNGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA58805.89148840238465No Hit
NNTNGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA47104.719202444767296No Hit
NNNNGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA15791.5820850658784629No Hit
NNNAGNGGTGAGCGGNTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA15051.50794048394369No Hit
NNNAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA14931.495917038224538No Hit
NNTAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA14591.4618506086869396No Hit
NATAGAGGTGAGCGGNTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA11011.103151144732228No Hit
NNTAGNCCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG8480.8496568308200992No Hit
NNNAGNCCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG7650.7664946645959622No Hit
NNTAGNCCAGCTTTGCATGAGTGTGGTTAATGAAGCTCTTCCATGTCCCA7320.7334301888682931No Hit
NNNNGNCCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG7250.7264165121987877No Hit
NNNNGNCCAGCTTTGCATGAGTGTGGTTAATGAAGCTCTTCCATGTCCCA7160.7173989279094234No Hit
NNNAGNCCAGCTTTGCATGAGTGTGGTTAATGAAGCTCTTCCATGTCCCA6990.7003657131406242No Hit
NATAGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG6230.6242172235859927No Hit
NATAGACCAGCTTTGCATGAGTGTGGTTAATGAAGCTCTTCCATGTCCCA6190.6202094083462752No Hit
NNTNGNCCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG6080.6091879164370522No Hit
NNTAGNTACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG6050.6061820550072642No Hit
NNNAGNTACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG5860.5871449326186062No Hit
NNTNGNCCAGCTTTGCATGAGTGTGGTTAATGAAGCTCTTCCATGTCCCA5590.5600921797505135No Hit
NNNNGNTACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG5340.5350433345022794No Hit
NNTAGNCACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT4940.49496518210510493No Hit
NNTNGNTACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG4780.4789339211462351No Hit
NNNAGNCACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT4690.46991633685687084No Hit
NATAGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG4680.46891438304694155No Hit
NNNNGNCACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT4670.4679124292370122No Hit
NNNAGNTGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT4170.41781473874054403No Hit
NNTAGNTGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT4070.4077952006412504No Hit
NATAGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT3990.39977957016181553No Hit
NNTNGNCACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT3820.3827463553930164No Hit
NNNNGNTGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT3820.3827463553930164No Hit
NATAGATGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT3350.33565452632633636No Hit
NNTNGNTGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT3210.3216271729873253No Hit
NNTAGNTGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG3140.31461349631781976No Hit
NNTAGNCACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG2520.2524923601021993No Hit
NNNAGNCACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG2510.25149040629226993No Hit
NNNAGNTGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG2470.24748259105255246No Hit
NNTAGNCCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG2350.23545914533340015No Hit
NNNNGNTGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG2350.23545914533340015No Hit
NATAGATGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG2270.22744351485396525No Hit
NNNNGNCACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG2200.22042983818445971No Hit
NNNNGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG2180.218425930564601No Hit
NNNAGNCCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG2070.207404438655378No Hit
NNNAGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG2060.20640248484544862No Hit
NNNNGACCAGCTTTGCATGAGTGTGGTTAATGAAGCTCTTCCATGTCCCA2030.20339662341566056No Hit
NNTNGNTGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG2010.2013927157958018No Hit
NATAGACACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG1920.19237513150643754No Hit
NNNNGNCCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG1880.1883673162667201No Hit
NNTNGNCACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG1880.1883673162667201No Hit
NNTAGACCAGCTTTGCATGAGTGTGGTTAATGAAGCTCTTCCATGTCCCA1850.185361454836932No Hit
NNNNGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG1850.185361454836932No Hit
NNNAGNCCTCCTTTCNTCCACGGCAAACACTCAAAACAGCCCTCTATGTG1850.185361454836932No Hit
NATAGACCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG1820.1823555934071439No Hit
NNTAGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG1760.17634387054756775No Hit
NNNAGACCAGCTTTGCATGAGTGTGGTTAATGAAGCTCTTCCATGTCCCA1750.1753419167376384No Hit
NNTNGNCCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG1680.16832824006813285No Hit
NNNAGNTACGATCATNAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG1540.1543008867291218No Hit
NNNAGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG1530.15329893291919242No Hit
NNNAGNCCAGCTTTGNATGAGTGTGGTTAATGAAGCTCTTCCATGTCCCA1530.15329893291919242No Hit
NNTAGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG1460.14628525624968688No Hit
NNNNGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT1310.13125594910074645No Hit
NNTAGNGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA1270.127248133861029No Hit
NNNNGATGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT1230.12324031862131156No Hit
NNNAGNGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA1210.12123641100145284No Hit
NNTAGNCCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC1210.12123641100145284No Hit
NNNAGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT1160.11622664195180601No Hit
NNNAGNCACCATTGGNGACCTGAGCATCCCTCATTACTCTGGTTCTATGT1110.11121687290215922No Hit
NNNAGNCCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT1080.10821101147237112No Hit
NNNAGNTGTTTGAAANTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT1080.10821101147237112No Hit
NNTAGATGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT1070.10720905766244176No Hit
NNNNGNCCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC1060.1062071038525124No Hit
NNTAGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT1040.10420319623265367No Hit
NNNAGNCCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC1020.10219928861279494No Hit
NNNAGATGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT1000.10019538099293622No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)