FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005804274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005804274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88216
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCTGCG1705319.33096037000091No Hit
CATAGAGAGATTTTGGGCTGAGACGATAATGCCGCATATCTACAACTATC72308.195792146549378No Hit
CATAGAAAACAGAGTCACAGTCCCCAGTCAATTCCCAGACATAAGCCAGT61676.990795320576766No Hit
CATAGAGAGATTTTGGGCTGAGACGATGGGGTTTTCTAGATATACAACAA34793.9437290287476197No Hit
CATAGACACCATGCCTGGCTAATTTTTGTATTTTTAGTAGAGATAGGGTT32083.636528520903237No Hit
CATAGACACCATGCCTGGCTAATTTTTTGCGTTTTTAGTAGAGATGGGGT26242.9745170944046433No Hit
CATAGACCTGGGCTTAGGGGCTACACATGGCTATGCAATCTATGAGACCA19242.181010247574136No Hit
CATAGAACTTCACAAGGGCTTTAGGGGCTACACATGGCTATGCAATCTAT17902.029110365466582No Hit
CATAGACCTGGGCTTAGGGGCTATACATGGCTATGCAATCTATGAGACCA17662.001904416432393No Hit
CATAGAAAACAGAGTCACAGTCCCCAGTAATGTTAGCATGTCTATGAGAC14571.651627822617212No Hit
CATAGATCCCATCCAATAATAATGAGCAAGCAATGGGCAAGCAATGACAG14011.588147274870772No Hit
CATAGAGAGATTTTGGGCTGAGACGATGGGGTTTTCTAGATATACACTCA12191.38183549469484No Hit
CATAGAGAGATTTTGGGCTGAGACGATGGGGTTTTCTAGATATACAATCA12021.3625646141289562No Hit
CATAGATCCCATCCAATAATAATGAGCAAGCAATGGGGAAAGGATTCTAT9811.1120431667724675No Hit
CATAGAGAGATTTTGGGCTGAGACGATGAGGTTTTCTAAATATACAATCA9071.0281581572503855No Hit
CATAGATGTCCATCATGTGGAAGCTGGAGAGTCACTTCTTTTGATAATTC7480.8479187448988845No Hit
CATAGATGCCCTTTTTGTGTACTTCCACACTGACGACATGGTTCTACACA7070.801441915298812No Hit
CATAGATCATGGGACTTCAGCATGGCCAACATGGTGAACCTGTCTCTACT6940.7867053595719598No Hit
CATAGACACCATGCCTGGCTAATTTTTGTATTTTTAGTAGAGACAGCGTT6100.6914845379522989No Hit
CATAGACACCATGCCTGGCTAATTTTTGTATTTTTAGTAGTGGTGTGGTG6100.6914845379522989No Hit
CATAGACACCATGCCTGGCTAATTTTAAAATTAGTACCTATAATCTCAGC5840.6620114264985943No Hit
CATAGAAACCCCAAAGGCTATGTTCAAAATGGTCAAACAGGAGGTCTATG5650.6404733835131949No Hit
CATAGACACCATGCCTGGCTAATTTTTGTATTTTTTGTAGAGACAGAGTT5140.5826607418155436No Hit
CATAGATGTCAAGAGTCTAATTCCTGCTCTATGTCTGTTATTCAAAGGTT4960.562256280039902No Hit
CATAGATGTATACAAGCTGTCACAGACAACAAGCAATGGGGAAAGGATTC4820.5463861431032919No Hit
CATAGACACCATGCCTGGCTAATTTTTTGTATTTTTAGTAGAGATGGGGT4270.4840391765666092No Hit
CATAGACACCATGCCTGGCTAATTTTTTGTATTAATCCCAGCTACTCAGG3970.4500317402738732No Hit
CATAGACCTGGGCTTAGGGGCTATACGTGAATGTGGATCAGAACTTCTAT3890.4409630905958103No Hit
CATAGAGGATCCCATCTGAGTTGGTCAAAGTCTGTGAGTAAAGTGCTAAT3740.42395937244944226No Hit
CATAGATCCCATCCAATAATAATGAACAAGCAATGGGGAAAGGATTCTAT3450.39108551736646413No Hit
CATAGATTTGTAAATTGCCTAGTCTTGCATATGTCTTTATCAGCAGCATT3450.39108551736646413No Hit
CATAGAAAACAGAGTCACAGTCCCCAGTATCCCCAGAAATGTTAGCATGT3170.35934524349324387No Hit
CATAGATTCTGAAGAAATCAGATAGAGAGAAACAAATATGCTCCAAATTT3160.358211662283486No Hit
CATAGAAAACAGAGTCACAGTCCCCAGTAATGTTAGTATCCCCAGAAATG3130.3548109186542124No Hit
CATAGACAGGGAGGCGTATAAGTGATGATCCTTTCCCTCACTCTCTCCAT3130.3548109186542124No Hit
CATAGATGTCAAGAGTCTAATTCCTGCTTCCTTCTGCTGGCTATTCTATG3050.34574226897614946No Hit
CATAGATGCCCTTTTTGTGTACTTCCACACTGTACCATGTTTTCATCCTG3040.3446086877663916No Hit
CATAGATGCCCTTTTTGTGTACTTCCAACACTGATCTATCCAGGAATTTT2990.3389407817176023No Hit
CATAGATGCCCTTTTTGTGTACTTCCATGTGCTTCTTTCAAAAGTCTGGT2840.32193706357123425No Hit
CATAGATGTCAAGAGTCTAATTCCTGCTCTCTGTTATTCAAAGGTTTTCT2600.2947311145370454No Hit
CATAGAGCTGGCTTCCTAACTTCCAAATCCTATCCCCAGAAATGTTAGCA2480.281128140019951No Hit
CATAGACACCATGCCTGGCTAATTTTTAAATATTTTGTAGAGACCGGGTT2420.27432665276140383No Hit
CATAGATCATGGGACTTCAGCATGGCATAGATGGAGACAGCCGCTCACCT2410.27319307155164596No Hit
CATAGACCAATAAAAGTTGATGGGATGAAGTAAGAAACATTTTAGAGCAC2290.25959009703455155No Hit
CATAGAGAGATTTTGGGCTGAGACGATGGCGTATTCTAAATATACAATCA2250.25505577219552006No Hit
CATAGATCATGGGACTTCAGCATGGCCAACATGGTGAAACCCCGTCTCTA2200.24938786614673078No Hit
CATAGATGCCCTTTTTGTGTACTTCCAACACTGACTGATACAGGAAGGCC2090.2369184728393942No Hit
CATAGACACCATGCCTGGCTAATTTTTAAAAAGTAGCTGGGCATGGTGGC2050.23238414800036278No Hit
CATAGACACCATGCCTGGCTAATTTTTGTATTTTTAGTGGTGCACATGCC1940.2199147546930262No Hit
CATAGATTTGTAAATTGCCTAGTCTTGCATACACAAAGTCACGAAAGTGG1890.21424684864423688No Hit
CATAGACACCATGCCTGGCTAATTTTGTATTTTTAGTAGAGACAGGGTTT1740.19724313049786887No Hit
CATAGACCTTTGGGTTCATATCATCTGGACACTGACGACATGGTTCTACA1740.19724313049786887No Hit
CATAGAGCTGGCTTCCTAACTTCCAATGGGCAAGCAATGACAGTCTATGA1660.18817448081980592No Hit
CATAGACCTTTGGGTTCATATCATCTGGCTACACATGGCTATGCAATCTA1610.1825065747710166No Hit
CATAGACACCATGCCTGGCTAATTTTTTTGTGTTTTTGGTAGAGATGGGG1610.1825065747710166No Hit
CATAGACCTTTGGGTTCATATCATCTGGAGACAGCCGCTCACCTCTATGA1560.17683866872222725No Hit
CATAGATCCCATCCAATAATAATGAGCAAGCAATGACAGTCTATGAGACC1550.17570508751246938No Hit
CATAGAACTTCACAAGGGCTTTAGGGCTACACATGGCTATGCAATCTATG1450.16436927541489071No Hit
CATAGATGTCAAGAGTCTAATTCCTGCTCTATGAGACCAAGAATCTGTGA1430.162102112995375No Hit
CATAGATGAAAGCCTGTTGAATTTATGAGACCAAGTCTCTGCTACCGTAC1370.1553006257368278No Hit
CATAGACACCATGCCTGGCTAATTTTTGTATTTTTAGTAGAGACAGGGTT1360.15416704452706992No Hit
CATAGAGAGATTTTGGGCTGAGACGATGGGGTTCTCTAAATATACACTCA1270.14396481363924912No Hit
CATAGATGTCAAGAGTCTAATTCCTGCTCCCGTACAATGGGCAAGCAATG1230.13943048880021763No Hit
CATAGAGCTGGCTTCCTAACTTCCAACTGCCTTCCTAACCATGGGCATAT1220.1382969075904598No Hit
CATAGATCATGGGACTTCAGCATGGCCAACATGGTGAAACCCCATCTCTA1200.13602974517094404No Hit
CATAGATGCCCTTTTTGTGTACTTCCACTGACGACATGGTTCTACACATA1190.13489616396118617No Hit
CATAGATGTCAAGAGTCTAATTCCTGCTCTATCTGTTATTCAAAGGTTTT1130.12809467670263897No Hit
CATAGATCATGGGACTTCAGCATGGCCAACATGGTGAAACCCTGTCTCTA1120.12696109549288112No Hit
CATAGAGCCAAGGAAAGTTGAATGGAGACAGCCGCTCACCTCTATGAGAC1080.12242677065384963No Hit
CATAGATCCCATCCAATAATAATGAGCAATGGGCAAGCAATGACAGTCTA1060.1201596082343339No Hit
CATAGAGATGTTTGATAAGCAGGGTGAAGCCTGGTCTCTACTAAAAGTAC1020.11562528339530244No Hit
CATAGACACCATGCCTGGCTAATTTTTTGTATTTTTCGTAGAGACAGGGT1020.11562528339530244No Hit
CATAGATCATGGGACTTCAGCATGGCGATGTTTGCAGATTTGGACCTGCG1020.11562528339530244No Hit
CATAGATGAAAGCCTGTTGAATTTATGAGACACAAGCAATGGGGAAAGGA950.10769021492699737No Hit
CAAAGTCACGAAAGTGGGTCTATGAGACCAAGTCTCTGCTACCGTACGAG950.10769021492699737No Hit
CATAGATGTCAAGAGTCTAATTCCTGCTCCGTATCAAAATGGTCAAACAG920.10428947129772377No Hit
CATAGATCATGGGACTTCAGCATGGCCAACGTGGTGAAAACCTGTCTCTA900.10202230887820804No Hit
CATAGATCCTCTCATTGTCCAGTTTGGCTACACATGGCTATGCAATCTAT890.10088872766845017No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)