FastQCFastQC Report
Tue 7 May 2024
EGAF00005804281

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005804281
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34164
Total Bases5.1 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC35

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGATTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA27848.148928696873902No Hit
CATAGAATCCTTTCCCCATTGCTTGCTCATTATTATTGGATGGGATCTAT26647.797681770284511No Hit
CATAGATTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT26497.753775904460836No Hit
CATAGATTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA24847.2708113804004215No Hit
CATAGAATCCTTTCCCCATTGCTTGTTCATTATTATTGGATGGGATCTAT18915.535066151504508No Hit
CATAGACTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA18595.441400304414003No Hit
CATAGAATAGCCAGCAGAAGGAAGCAGGAATTAGACTCTTGACATCTATG14494.241306638566913No Hit
CATAGACCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG8592.5143425828357335No Hit
CATAGAAAGGCCTTCCTGTATCAGTCAGTGTGGAAGTACACAAAAAGGGC7562.212855637513172No Hit
CATAGAAAGGCCTTCCTGTATCAGTCAGTGTTTGGAAGTTAGGAAGCCAG7162.095773328650041No Hit
CATAGAGGTGAGCGGCTGTCTCCATTCAACTTTCCTTGGCTCTATGTGTA5761.685985247629083No Hit
CATAGACATGCTAACATTTCTGGGGATAACTTGGTCTCATAGACATGCTA5671.6596417281348788No Hit
CATAGACCTCCTGTTTGACCATTTTGAACATAGCCTTTGGGGTTTCTATG5321.5571947078796393No Hit
CATAGATGGAGAGAGTGAGGGAAAGGATTCTATTTGGAAGTTAGGAAGCC4201.2293642430628733No Hit
CATAGAAGTTCTGATCCACATTCACGTATAGCCCCTAAGCCCAGGTCTAT3791.109354876478164No Hit
CATAGACTGTCATTGCTTGCCCATTGCTCATTATTATTGGATGGGATCTA3280.9600749326776724No Hit
CATAGAGGTGAGCGGCTGTCTCCAGATGATATGAACCCAAAGGTCTATGT3200.9366584709050463No Hit
CATAGACTTCCTTGCCCCCATTTATTTACGGGAGCAGGAATTAGACTCTT3070.8986067205245288No Hit
CATAGACATGCTAACATTTCTGGGGATAACATTACTGGGGACTGTGACTC2930.857627912422433No Hit
CATAGACATGCTAACATTACTGGGGACTGTGACTCTGTTTTCTATGTGTA2360.6907856222924716No Hit
CATAGACATGCTAACATTTCTGGGGATACTGGGGACTGTGACTCTGTTTT2270.6644421027982672No Hit
CATAGACCATCCAGAGGTAATTCTGCTCATTATTATTGGATGGGATCTAT2270.6644421027982672No Hit
CATAGACCAGACTTTTGAAAGAAGCACACAAAAAGGGTGGAAGTACACAA2020.5912656597588104No Hit
CATAGAAGCAGGATGAAAACATGGTACATTACTGGGGACTGTGACTCTGT2020.5912656597588104No Hit
CATAGACTGTCATTGCTTGCCCATTGCAGAGACTTGGTCTCATAGACTGT1760.5151621589977754No Hit
CATAAATTCAACAGGCTTTCATCTATGTGTAGAACCATGTCGTCAGTGTA1650.48296452406041446No Hit
CATAGATTGCATAGCCATGTGTAGCCAGATGATATGAACCCAAAGGTCTA1570.4595480622877883No Hit
CATAGATATGCCCATGGTTAGGAAGGCAGCTCTATGTGTAGAACTTGGAA1560.45662100456621No Hit
CATAGAAGTTCTGATCCACATTCACGTATAGCCCCTAAAGCCCTTGTGAA1560.45662100456621No Hit
CATAGATTGCATAGCCATGTGTAGCCCTAAAGCCCTTGTGAAGTTCTATG1490.4361316005151622No Hit
CATAGACATGCTAACATTTCTGGGGATATTACTGGGGACTGTGACTCTGT1450.424423369628849No Hit
CATAGATGGAGAGAGTGAGGGAAAGGATTCTATGAGACCAACTCAGATGG1420.41564219646411427No Hit
CATAGATATGCCCATGGTTAGGAAGGCAGTTGGAAGTTAGGAAGCCAGCT1270.3717363306404402No Hit
CATAGATTGCATAGCCATGTGTAGCCATGCTGAAGTCCCATGATCTATGT1270.3717363306404402No Hit
CATAGATTGAGCTTAAATGATCTGTGCAAGACTAGGCAATTTACAAATCT1160.33953869570307926No Hit
CATAGACTGTCATTGCTTGCCCATTGTTGCTTGCTCATTATTATTGGATG1140.3336845802599227No Hit
CATAGACATGCTAACATTTCTGGGGATTTACTGGGGACTGTGACTCTGTT1110.3249034070951879No Hit
CATAGAGCAGAAAACCTTTGAATAACAGAGCAGGAATTAGACTCTTGACA1100.32197634937360964No Hit
CATAGACTGTCATTGCTTGCCCATTGGACTGTCATTGCTTGCTCATTATT1100.32197634937360964No Hit
CATAGAGTTCAAATGGCCAATCAGGTATTCGGTGAGAACGATCTTGCTGG1080.31612223393045313No Hit
CATAGACATGCTAACATTTCTGGGGATACATTACTGGGGACTGTGACTCT980.2868516567146704No Hit
CATAGATATGCCCATGGTTAGGAAGGCCAGCTTCCACATGATGGACATCT940.2751434258283573No Hit
CATAGACATGCTAACATTTCTGGGGATTTGGTCTCATAGACATGCTAACA920.26928931038520076No Hit
CATAGACCAGACTTTTGAAAGAAGCACACAAAAAGGGCATGGAAGTACAC880.2575810794988877No Hit
CATAGATATGCCCATGGTTAGGAAGGCAGCTTCCACATGATGGACATCTA870.25465402177730945No Hit
CATAGACATGCTAACATTTCTGGGGATGACTTGGTCTCATAGACATGCTA830.24294579089099635No Hit
CATAGACATGCTAACATTTCTGGGGATACGGTAGTAGAGACTTGGTCTCA700.20489404051047885No Hit
CATAGATGAAAAGTCAAGCCAGCACAAAATCTCTCTCGTCTCAGCCCAAA640.18733169418100926No Hit
CATAGACATGCTAACATTTCTGGGGATACGGTAGCAGAGACATGGTCTCA640.18733169418100926No Hit
CATAGACATGCTAACATTTCTGGGGATATACGGTAGCAGAGACTTCGTCT610.17855052101627444No Hit
CATAGAGCAGGAATTAGACTCTTGACATCTATGTGTAGAACCATGTCGTC590.1726964055731179No Hit
CATAGAATAGCCAGCAGAAGGGAGCAGGAATTAGACTCTTGACATCTATG580.16976934785153963No Hit
CATAGACTGTCATTGCTTGCCCATTGTCATTGCTTGCTCATTATTATTGG540.15806111696522657No Hit
CATAGATTGAGCTTAAATGATCTGTGCAACCCCTTAAGAAAATTTAATTG540.15806111696522657No Hit
CATAGACCATCCAGAGGTAATTCTGCTACCGTACCCTAAAGCCCTTGTGA530.1551340592436483No Hit
CATAGACCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGGAAGT520.15220700152207No Hit
CATAGAATAGCCAGCAGAAGGAAGCAGCTTCCACATGATGGACATCTATG460.13464465519260038No Hit
CATAGACATGCTAACATTTCTGGGGATTGGTCTATAGACATGCTAACATT460.13464465519260038No Hit
CATAGACTGTCATTGCTTGCCCATTGTTATTGGAAGTTAGGAAGCCAGCT460.13464465519260038No Hit
CATAGAAAGGCCTTCCTGTATCAGTCAGTGTTTGGAAGTACACAAAAAGG450.13171759747102213No Hit
CATAGATGAAAAGTCAAGCCAGCACAAGGGAGCAGGAATTAGACTCTTGA430.1258634820278656No Hit
CATAGACTGTCATTGCTTGCCCATTGACTGTCATTGCTTGCTCATTATTA430.1258634820278656No Hit
CATAGACATGCTAACATTTCTGGGGACTGTGACTCTGTTTTCTATGTGTA380.11122819341997424No Hit
CATAGATATGCCCATGGTTAGGAAGGCTGGCAAAGTTCCAATCTATGTGT370.10830113569839597No Hit
CATAGACATGCTAACATTTCTGGGGATATAGACATGCTAACATTACTGGG370.10830113569839597No Hit
CATAGACATGCTAACATTTCTGGGGATATGCTAACATTACTGGGGACTGT370.10830113569839597No Hit
CATAGAAAGGCCTTCCTGTATCAGTCAGTGTACTGGGGACTGTGACTCTG360.10537407797681769No Hit
CATAGACTGTCATTGCTTGCCCATTGTTTGGAAGTTAGGAAGCCAGCTCT350.10244702025523943No Hit
CATAGACATGCTAACATTTCTGGGGATCTAACATTACTGGGGACTGTGAC350.10244702025523943No Hit

[FAIL]Adapter Content

Adapter graph