FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005804303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005804303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59032
Sequences flagged as poor quality0
Sequence length150
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNAGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA15132.5630166689253286No Hit
NNNNGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA15032.546076704160455No Hit
NNTAGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA14702.4901748204363736No Hit
NATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA14152.3970050142295705No Hit
NNTAGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA13312.254709310204635No Hit
NNNNGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA12782.1649274969508063No Hit
NNNAGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA12702.1513755251389077No Hit
NATAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA11611.966729909201789No Hit
NNTNGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA11371.9260739937660931No Hit
NNTNGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA9741.6499525680986584No Hit
NNNAGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT5360.9079821113972084No Hit
NNTAGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT5070.8588562135790758No Hit
NNNNGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT4550.7707683968017347No Hit
NATAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT4460.7555224285133487No Hit
NNTAGNTTGAGCTTAAATGATCTGTGCAAGGTTAAACAATATCCAAATCC4000.6775985905949315No Hit
NNNAGNTTGAGCTTAAATGATCTGTGCAAGGTTAAACAATATCCAAATCC3920.664046618783033No Hit
NNTNGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT3870.6555766364005963No Hit
NNNAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA3850.6521886434476216No Hit
NNNAGNGGTGAGCGGNTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA3830.648800650494647No Hit
NNTAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA3800.6437186610651849No Hit
NNNNGNTTGAGCTTAAATGATCTGTGCAAGGTTAAACAATATCCAAATCC3780.6403306681122103No Hit
NNNNGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA3670.6216967068708497No Hit
NNNNGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA3580.6064507385824637No Hit
NATAGATTGAGCTTAAATGATCTGTGCAAGGTTAAACAATATCCAAATCC3510.5945927632470525No Hit
NNTNGNTTGAGCTTAAATGATCTGTGCAAGGTTAAACAATATCCAAATCC3250.5505488548583819No Hit
NNNAGNATCCTTTCCNCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA3140.5319148936170213No Hit
NNNAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA3080.5217509147580973No Hit
NNTAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA3030.5132809323756606No Hit
NNNAGNTTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT2750.4658490310340154No Hit
NNTAGNTTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT2690.4556850521750915No Hit
NNTAGNTTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA2380.4031711614039843No Hit
NNTAGNTTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA2330.39470117902154767No Hit
NNNAGNTTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA2260.38284320368613634No Hit
NNNAGNTTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA2260.38284320368613634No Hit
NATAGAGGTGAGCGGNTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA2240.3794552107331617No Hit
NNNNGNTTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA2210.3743732213036997No Hit
NNNNGNTTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA2170.36759723539775035No Hit
NNNNGNTTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT2090.3540452635858517No Hit
NNNAGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGAAAG2030.34388128472692775No Hit
NNTNGNTTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT2010.3404932917739531No Hit
NATAGATTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA1940.3286353164385418No Hit
NATAGATTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT1920.3252473234855671No Hit
NNTAGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGAAAG1920.3252473234855671No Hit
NNTAGNCTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA1880.3184713375796179No Hit
NATAGATTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA1860.31508334462664317No Hit
NNTNGNTTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA1850.31338934815015584No Hit
NNNAGNCTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA1840.3116953516736685No Hit
NATAGAATCCTTTCCNCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA1770.29983737633825724No Hit
NNNNGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT1650.2795094186204093No Hit
NNTAGNATCCTTTCCCCATTGCTTGCTCATTATTATTGGATGGGATCTAT1650.2795094186204093No Hit
NNTNGNTTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA1630.2761214256674346No Hit
NATAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGAAAG1530.25918146090256133No Hit
NNNAGNATCCTTTCCCCATTGCTTGCTCATTATTATTGGATGGGATCTAT1500.25409947147309936No Hit
NNNNGNATCCTTTCCCCATTGCTTGCTCATTATTATTGGATGGGATCTAT1460.24732348556715No Hit
NNTNGNCTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA1460.24732348556715No Hit
NNTNGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGAAAG1450.2456294890906627No Hit
NNNNGNCTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA1440.24393549261417535No Hit
NNNNGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGAAAG1380.23377151375525138No Hit
NATAGAATCCTTTCCCCATTGCTTGCTCATTATTATTGGATGGGATCTAT1370.23207751727876408No Hit
NATAGACTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA1350.2286895243257894No Hit
NNTAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT1300.22021954194335275No Hit
NNTAGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCAACATGGTG1280.21683154899037807No Hit
NNNAGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCAACATGGTG1250.2117495595609161No Hit
NNNNGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCAACATGGTG1250.2117495595609161No Hit
NATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCAACATGGTG1220.20666757013145412No Hit
NNNAGNATCCTTTCCNCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT1170.19819758774901747No Hit
NNTNGNATCCTTTCCCCATTGCTTGCTCATTATTATTGGATGGGATCTAT1130.19142160184306817No Hit
NNNNGATTGAGCTTAAATGATCTGTGCAAGGTTAAACAATATCCAAATCC1100.18633961241360616No Hit
NNNAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT1100.18633961241360616No Hit
NNNAGNTATGCCCATGGTTAGGAAGGAAGGATCAATATTGTTAAAATGGG1030.17448163707819486No Hit
NNTNGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCAACATGGTG1030.17448163707819486No Hit
NNTAGNCCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG1020.17278764060170757No Hit
NNNAGNCCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG1000.1693996476487329No Hit
NNNAGNTTGAGCTTANATGATCTGTGCAAGGTTAAACAATATCCAAATCC990.16770565117224556No Hit
NNTAGATTGAGCTTAAATGATCTGTGCAAGGTTAAACAATATCCAAATCC950.16092966526629623No Hit
NNTAGNTATGCCCATGGTTAGGAAGGAAGGATCAATATTGTTAAAATGGG950.16092966526629623No Hit
NNNNGNTATGCCCATGGTTAGGAAGGAAGGATCAATATTGTTAAAATGGG920.15584767583683426No Hit
NNNAGATTGAGCTTAAATGATCTGTGCAAGGTTAAACAATATCCAAATCC920.15584767583683426No Hit
NNNNGNCCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG870.1473776934543976No Hit
NNTAGNAAGGCCTTCCTGTATCAGTCAGTGTCATCACTTATACGCCTCCC840.14229570402493563No Hit
NNTNGNTATGCCCATGGTTAGGAAGGAAGGATCAATATTGTTAAAATGGG840.14229570402493563No Hit
NNNAGNAAGGCCTTCCTGTATCAGTCAGTGTCATCACTTATACGCCTCCC810.13721371459547363No Hit
NNTNGNCCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG790.133825721642499No Hit
NNNAGATTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT760.12874373221303698No Hit
NATAGACCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG750.12704973573654968No Hit
NNNNGNTGGAGAGAGTGAGGGAAAGGAAATTATGGGAGCCCTGTGAAACG730.123661742783575No Hit
NNNAGNCTGCCTCAGCCTCCTGAGTAGCTGGAATTACAGGTGCCCGCCAC720.12196774630708768No Hit
NNNNGNAAGGCCTTCCTGTATCAGTCAGTGTCATCACTTATACGCCTCCC720.12196774630708768No Hit
NATAGACTGCCTCAGCCTCCTGAGTAGCTGGAATTACAGGTGCCCGCCAC710.12027374983060037No Hit
NNNNGATTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA710.12027374983060037No Hit
NNNNGATTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT700.11857975335411303No Hit
NNNAGNTTGCATAGCNATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA690.11688575687762569No Hit
NNNAGATTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA680.11519176040113836No Hit
NNTAGNAGTTCTGATCCACATTCACGTATAGCCCCTAAGCCCAGGTCTAT670.11349776392465105No Hit
NNNAGNATCCTTTCCCCATTGCTTGTTCATTATTATTGGATGGGATCTAT660.11180376744816371No Hit
NNNAGNTGGAGAGAGTGAGGGAAAGGAAATTATGGGAGCCCTGTGAAACG660.11180376744816371No Hit
NATAGATATGCCCATGGTTAGGAAGGAAGGATCAATATTGTTAAAATGGG650.11010977097167637No Hit
NNTAGNCCAGACTTTTGAAAGAAGCACATCACTTATACGCCTCCCTGTCT640.10841577449518904No Hit
NNNNGNAGTTCTGATCCACATTCACGTATAGCCCCTAAGCCCAGGTCTAT640.10841577449518904No Hit
NNTAGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTAATACAAAAAATTAG640.10841577449518904No Hit
NNNAGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTAATACAAAAAATTAG630.10672177801870172No Hit
NNNAGATTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA630.10672177801870172No Hit
NNNAGNTTGCATAGCNATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA630.10672177801870172No Hit
NNTNGNAAGGCCTTCCTGTATCAGTCAGTGTCATCACTTATACGCCTCCC620.1050277815422144No Hit
NNNNGNCTGTCATTGCTTGCCCATTGCTTGTTTTTCTCAGGTTTGTCAAA620.1050277815422144No Hit
NNNNGATTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA620.1050277815422144No Hit
NNNAGNTTGCATAGCNATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT610.10333378506572706No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)