FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005804316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005804316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250218
Sequences flagged as poor quality0
Sequence length150
%GC35

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGAGCTTGGGTGCGAACATCAAGGAAGTTTCTGTGAGATCTATGAGA2564910.250661423238935No Hit
CATAGAGCTTTTTCTCCAGCCTCCAAGTAATAACCAGGCCCCTCTATGAG154556.176613992598455No Hit
CATAGACGGATTATTCTCTCCATGGTCTACCAGTTATTAGTTGAATGAGC141625.659864598070483No Hit
CATAGAGAAGACTCCCTCAACCCACCCACAGCCAGGAAAGAATCTATGAG133225.324157334804051No Hit
CATAGAAATGATGCTGAAGGGCCTGCCCTCTCTGCTGTTCCTTCTATGAG103154.122405262611003No Hit
CATAGATCACGTAGAAACTGGAGAGGCTAATCTGAAATCTTCGTGTTCTA100934.033682628747732No Hit
CATAGATGTTTTATCAAAGGCATGCTCATCAAGGAAGTTTCTGTGAGATC88543.538514415429745No Hit
CATAGAGGAGGTGAAAGAAATCTACAATGGGTTCTGGGCCCCAGTGTGAA71182.84471940467912No Hit
CATAGATCATGGGACTTCAGCATGGCTCAGGTATGTCTTTATTAGCTCTA70092.8011573907552614No Hit
CATAGACTCATTGCACTTTCCACAACTGTGTGCTTGAACCAGATTCTATG65032.598933729787625No Hit
CATAGACTCATTGCACTTTCCACAACTGTCATTGTCGCTGTCCCATCTAT56192.2456418003500946No Hit
CATAGATGTTTTATCAAAGGCATGCTCATCAGGAACCATGGGAGGTCTAT47891.9139310521225492No Hit
CATAGAAGTCACGAGTTTTGTTGTTGTTCTTCCTTTTCTGCCTTCATCTA47311.8907512648970097No Hit
CATAGACTCATTGCACTTTCCACAACTGTCCCAACTGTCTCTGAAAAATT46311.850786114508149No Hit
CATAGAAAATTTGTGATGGTAAATCATGGGACATGCCAGGAATCAATGTT41381.6537579230910644No Hit
CATAGATCAAGGAAAACACCAGCCCACGCTGTCCTTCTCTGAATCTATGA41151.6445659385016267No Hit
CATAGATGTTTTATCAAAGGCATGCTCATTTATTTTAAAGGACTGGAAGC39471.5774244858483402No Hit
CATAGAAGCTGAGAAGACTTCCTAATGCAGAAGTAGGAAGTATTACCCCT38211.5270683963583755No Hit
CATAGAAATGATGCTGAAGGGCCTGCTGAAGTGATGGGCTAGATCTATGA35231.40797224819957No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCTGCG25581.022308546947062No Hit
CATAGACCAGCAGACACTCCCATCATTTATTTTAAAGGACTGGAAGCCTC24670.9859402600931988No Hit
CATAGACTCATTGCACTTTCCACAACTGTCTGCCTCTAAGTAAACTGAAT22860.9136033378893604No Hit
CATAGAGTGCCTGTTTCCTCATTCGTCTGTTTCCCCACTCAAATACCATC22830.9124043833776947No Hit
CATAGAAATGATGCTGAAGGGCCTGTTTCCCCACTCAAATACCATCTATG22720.90800821683492No Hit
CATAGAGTCTGAATGGTGGGAGAGTGAAAGAAATCTACAAAGGATTCTGG21200.8472611882438514No Hit
CATAGACGTGACTAAAAGCATGTTGGTGGAAGGGTAAGAGAGGGTCTATG17820.7121789799295014No Hit
CATAGACCTCCGACAGTTTTTATGCACCTTCATTGTTGCTCTATGAGACC11960.47798319865077654No Hit
CATAGAGGAGGTGAAAGAAATCTACAATGGGCCCCAGTGTGAATACTTCT11850.4735870321080018No Hit
CATAGACCAACATTTCTTCTCCCAGGGTACACTGCCCTCTCTGCTGTTCC11260.4500075933785739No Hit
CATAGATGGCCAAGATACGAGCACATAATGATGCTGAAGTGATGGGCTAG10460.4180354730674851No Hit
CATAGAAGGACAAAGACATTTAGGACCCACGCTGTCCTTCTCTGAATCTA9660.3860633527563964No Hit
CATAGAAATGATGCTGAAGGGCCTGCGATGCTGAAGTGATGGGCTAGATC8990.3592867019958596No Hit
CATAGAGAAGACTCCCTCAACCCACGCTGTCCTTCTCTGAATCTATGAGA8740.34929541439864437No Hit
CATAGATCATGGGACTTCAGCATGGCTACCCTTCCCCACACCCTCTTCTT8670.3464978538714241No Hit
CATAGAAATGATGCTGAAGGGCCTGCTGCTGAAGTGATGGGCTAGATCTA8530.3409027328169836No Hit
CATAGAGCTTTTTCTCCAGCCTCCAAGGGGTAAGGCGTACTGGTCTATGA8390.3353076117625431No Hit
CATAGACCCAAGACCCCAGCCTTTACGATCATTGTTCACTGCAGCCTCTA8240.32931283920421395No Hit
CATAGATGTGTAGCCTGGGTCTGACATGCCAGGAATCAATGTTCTATGAG8210.3281138846925481No Hit
CATAGACCAGCAGACACTCCCATCATCAAGGAAGTTTCTGTGAGATCTAT8100.3237177181497734No Hit
CATAGATGTTTTATCAAAGGCATGCTCATACACTGACGCTATACCACACT7670.30653270348256323No Hit
CATAGACACAACAGAGAGAACGGGCTAGATGCTGAAGTGATGGGCTAGAT7340.2933442038542391No Hit
CATAGATGTTTTATCAAAGGCATGCTCATGGGTCTACCAGTTATTAGTTG7330.2929445523503505No Hit
CATAGAGCTTGGGCGCGAACATCAAGGAAGTTTCTGTGAGATCTATGAGA7150.28575082528035556No Hit
CATAGAAGGACAAAGACATTTAGGACCCAGGCAAGGGGTAAGGCGTACTG7040.2813546587375808No Hit
CATAGACACAACAGAGAGAACGGGCTACACTGGCTCAGGTATGTCTTTAT6950.27775779520258337No Hit
CATAGATCCACGTGAGAATAAAGCATACAACACTGTCATTGTCGCTGTCC6880.27496023467536307No Hit
CATAGAGTAGTTAAAACCCACAATGACAGTGTGGTGGAAGGGTAAGAGAG6850.2737612801636973No Hit
CATAGAGTAGTTAAAACCCACAATGACAGTGTCAAGGGGTAAGGCGTACT6840.2733616286598086No Hit
CATAGAAAAAACAGTTGGCCAGGCATCCGATCATTGTTCACTGCAGCCTC6710.26816615910925673No Hit
CATAGATGTTTTATCAAAGGCATGCTCATACACTGACGATCATTGTTCAC6550.261771735047039No Hit
CATAGAAATGATGCTGAAGGGCCTGCTACCGTACAAGGGGTAAGGCGTAC6420.25657626549648704No Hit
CATAGAGAAGACTCCCTCAACCCACCATTACGCTATACCACACTACACAC6320.25257975045760095No Hit
CATAGACGTGACTAAAAGCATGTTGGTCTACCAGTTATTAGTTGAATGAG6210.24818358391482626No Hit
CATAGATGGCCAAGATACGAGCACATACACTGACGCTATACCACACTACA6110.2441870688759402No Hit
CATAGATCCACGTGAGAATAAAGCATACACACTGACGATCATTGTTCACT6050.24178915985260852No Hit
CATAGAGCTGTACTGCATCTTTTCTCAAACTGCTGGGGTTGCTCTATGAG5560.2222062361620667No Hit
CATAGAAAAAACAGTTGGCCAGGCATCTATGCACCCACAGCCAGGAAAGA5520.22060763014651225No Hit
CATAGAAGCCATGAACACAGGATTGAGGAGCTGAGTCATGGGGTCTATGA5210.20821843352596536No Hit
CATAGACCAACATTTCTTCTCCCAGGGTGGAAGGGTAAGAGAGGGTCTAT5180.20701947901429954No Hit
CATAGAGTCTGAATGGTGGGAGAGTGAAGTTCTGCTTGTGGTGAGTTGAC5150.2058205245026337No Hit
CATAGACCTCCGACAGTTTTTATGCACTGTCCCAACTGTCTCTGAAAAAT5060.20222366096763622No Hit
CATAGAAATGATGCTGAAGTGATGGGCTAGATCTATGAGACCAAGTCTCT4810.19223237337042098No Hit
CATAGACACAACAGAGAGAACGGGCTCCGAGGTCAGGAAGGAACCTGCTC4700.1878362068276463No Hit
CATAGACTCATTGCACTTTCCACAACTGTACACTGACGCTATACCACACT4380.1750473587032108No Hit
CATAGAGCTTTTTTTCCAGCCTCCAAGTAATAACCAGGCCCCTCTATGAG4310.17224979817599054No Hit
CATAGACACAACAGAGAGAACGGGCTCTGGGCCCCAGTGTGAATACTTCT4230.16905258614488167No Hit
CATAGACCTCCGACAGTTTTTATGCACTGTCATTGTCGCTGTCCCATCTA4140.16545572260988417No Hit
CATAGACGGATTACTCTCTCCATGGTCTACCAGTTATTAGTTGAATGAGC4040.16145920757099808No Hit
CATAGAGAAGACTTCCTCAACCCACCCACAGCCAGGAAAGAATCTATGAG3910.15626373802044616No Hit
CATAGATCAAGGAAAACACCAGCCCACCCACAGCCAGGAAAGAATCTATG3880.15506478350878034No Hit
CATAGACTCATTGCACTTTCCACAACTGTACACTGACGATCATTGTTCAC3660.14627245042323095No Hit
CATAGAGTCTGAATGGTGGGAGAGTGAGCAGCATAATTTCCCATGTCATC3500.1398780263610132No Hit
CATAGACGTGACTAAAAGCATGTTGGTCAGTGTAGTACACTGACGATCAT3300.13188499628324102No Hit
CATAGACGTGACTAAAAGCATGTTGGTGCATTACAGAGAGGAGATCTATG3280.1310856932754638No Hit
CATAGAAATGATGTTGAAGGGCCTGCCCTCTCTGCTGTTCCTTCTATGAG3080.12309266319769162No Hit
CATAGAGCTGTACTGCATCTTTTCTCAGACCAGTGAAGCAGAGCTTCCCA3030.12109440567824857No Hit
CATAGACGGATTATTCTCTCCATGGTCTACACTGACGCTATACCACACTA2860.11430033011214222No Hit
CATAGACCAACATTTCTTCTCCCAGGGTACACTGACGACATGGTTCTACA2780.11110311808103335No Hit
CATAGATGTTTTACCAAAGGCATGCTCATCAAGGAAGTTTCTGTGAGATC2660.10630730003437003No Hit
CATAGACTCATTGCACTTTCCACAACTGTCTCTGAAGTCCCAACTGTCTC2550.10191113349159533No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)