FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005804331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005804331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137673
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA3531025.647730491817565No Hit
CATAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA102017.409586483914783No Hit
CATAGAGGTGAGCGGCTGTCTCCATTCAACTTTCCTTGGCTCTATGTGTA39752.8872763722734307No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGAATTACAGGTGCCCGCCAC38232.776869829233038No Hit
CATAGAGGTGAGCGGCTGTCTCCATGCTGAAGTCCCATGATCTATGTGTA30542.218299884508945No Hit
CATAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT24751.7977381185853434No Hit
CATAGATTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA21891.5899994915488147No Hit
CATAGATTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA21651.5725668794898056No Hit
CATAGATTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT21161.5369752965359946No Hit
CATAGAAAGGCCTTCCTGTATCAGTCAGTGTGGAAGTACACAAAAAGGGC19681.42947418883877No Hit
CATAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGAAAG15391.1178662482839772No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGGTCAGGAGATTGAGACCATCCTG13140.9544355102307642No Hit
CATAGAATCCTTTCCCCATTGCTTGCTCATTATTATTGGATGGGATCTAT12800.9297393098138342No Hit
CATAGATTGCATAGCCATGTGTAGCCATGCTGAAGTCCCATGATCTATGT12100.8788941913083902No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT10530.764855854089037No Hit
CATAGAGGTGAGCGGCTGTCTCCAGATGATATGAACCCAAAGGTCTATGT9790.7111053002404247No Hit
CATAGACATGCTAACATTTCTGGGGATACTGGGGACTGTGACTCTGTTTT9000.6537229522128523No Hit
CATAGACCTCCTGTTTGACCATTTTGAACATAGCCTTTGGGGTTTCTATG8710.6326585459748825No Hit
CATAGAATAGCCAGCAGAAGGAAGCAGGAATTAGACTCTTGACATCTATG6520.4735859609364218No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTGCCTGCCAC5500.39949735968563194No Hit
CATAGACTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA5160.3748011592687019No Hit
CATAGAATCCTTTCCCCATTGCTTGTTCATTATTATTGGATGGGATCTAT5000.363179417896029No Hit
CATAGACATGCTAACATTTCTGGGGATATTACTGGGGACTGTGACTCTGT5000.363179417896029No Hit
CATAGAGGTGAGCGGCTGTCTCCACTAAAAATACAAAAATTAGCCAGGCA4930.3580949060454846No Hit
CATAGAGGTGAGCGGCTGTCTCCAGCCATGCTGAAGTCCCATGATCTATG4710.3421150116580593No Hit
CATAGAGCAGAAAACCTTTGAATAACAGATAGAGCAGGAATTAGACTCTT4380.3181451700769214No Hit
CATAGAGGTGAGCGGCTGTCTCCAAACTGGACAATGAGAGGATCTATGTG4210.30579706986845645No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGTACCACAGGCATGTGCCAC4190.3043443521968723No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTGCCCGCCAC4120.29925984034632785No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGACTACAGGTGCCTGCTAT4070.2956280461673676No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGTGCCAC4010.29126989315261526No Hit
CATAGACTGTCATTGCTTGCCCATTGCTTGTTTTTCTCAGGTTTGTCAAA4010.29126989315261526No Hit
CATAGAAGTTCTGATCCACATTCACGTATAGCCCCTAAGCCCAGGTCTAT3930.2854590224662788No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTGCCAC3900.2832799459589026No Hit
CATAGATTTAAAATAAAATTCCTGGATAGATCATTTAGCCATGCTGAAGT3630.26366825739251704No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGACTACAGGTGCACACCAC3560.2585837455419726No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGACTGCAGGCGCATGCTTG3460.2513201571840521No Hit
CATAGACTGTCATTGCTTGCCCATTGACACTGACGCCATGCTGAAGTCCC3390.24623564533350767No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGACGACAGCCCGGCTACTG3360.24405656882613147No Hit
CATAGAATCCTTTCCCCATTGCTTGTTTTTGTCAGGTTTGTCAAAGATCA2880.20919134470811268No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTGCCCACCAC2690.19539052682806363No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGCGCCAC2560.18594786196276686No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCTTGTGTCAC2360.1714206852469257No Hit
CATAGAGGTGAGCGGCTGTCTCCAAGTCCGCGCAGAGCCTTCAGGTCAGA2320.16851524990375746No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTGTGCACCAC2150.15616714969529247No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCCACCAC2120.1539880731879163No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCG2010.14599812599420367No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCGCCTGCCAC1940.14091361414365924No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCGGGTCA1890.13728181996469896No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGAGATTATAGGTACTAATTTT1880.1365554611289069No Hit
CATAGACATGCTAACATTTCTGGGGATAATTACTGGGGACTGTGACTCTG1850.13437638462153073No Hit
CATAGAATCCTTTCCCCATTGCTTGTTTTTGTCAAATTTGTTAAAAGAGG1830.13292366694994662No Hit
CATAGATATGCCCATGGTTAGGAAGGGTTTGGAAGTTAGGAAGCCAGCTC1770.12856551393519428No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGCCA1740.12638643742781808No Hit
CATAGAGGTGAGCGGCTGTCTCCATCTATGAGACCAACTCAGATGGGATC1730.12566007859202605No Hit
CATAGAGGTGAGCGGCTGTCTCCACTAAAAATACAAAAAATTAGCCAGGC1720.12493371975623399No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGCACCAC1720.12493371975623399No Hit
CATAGAGGTGAGCGGCTGTCTCCAGGACAGAGGAGAACTCGTGCCTGGAA1670.12130192557727368No Hit
CATAGAGGTGAGCGGCTGTCTCCACGAGTCCGCGCAGAGCCTTCAGGTCA1640.11912284906989751No Hit
CATAGAGGTGAGCGGCTGTCTCCACTAAAAATACAAAATTAGCCAGGCAT1630.11839649023410545No Hit
CATAGATGGAGAGAGTGAGGGAAAGGATTCTATTCGTCTCAGCCCAAAAT1610.11694377256252134No Hit
CATAGATATGCCCATGGTTAGGAAGGCAGCTTCCACATGATGGACATCTA1610.11694377256252134No Hit
CATAGAGTTCAAATGGCCAATCAGGTAAGGCTGGCAAAGTTCCAATCTAT1590.11549105489093722No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCAACATGGTG1580.11476469605514515No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCGCCCGCCAC1580.11476469605514515No Hit
CATAGATTGCATAGCCATGTGTAGCCAGATGATATGAACCCAAAGGTCTA1540.11185926071197692No Hit
CATAGAGGTGAGCGGCTGTCTCCACAGGTCCGCGCAGAGCCTTCAGGTCA1540.11185926071197692No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCGGAGCCTTCAGGTCA1500.1089538253688087No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCTAATTTTTG1480.1075011076972246No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTCCAGGTCA1480.1075011076972246No Hit
CATAGACCATCCAGAGGTAATTCTGCTCATTATTATTGGATGGGATCTAT1460.10604839002564047No Hit
CATAGAAAGGCCTTCCTGTATCAGTCAGTGTTCATAAATTCAACAGGCTT1450.10532203118984841No Hit
CATAGACTGCCTCAGCCTCCTGAGTATCTGGGATTACAGGCATGCGCCAT1450.10532203118984841No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGACTACAGGTGTGTGCCAT1440.10459567235405634No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGACTGCAGGTGTGTGCCAC1440.10459567235405634No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCCTCAGGTCA1390.10096387817509606No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTAATACAAAAAATTAG1380.10023751933930401No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)