FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005804354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005804354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51422
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCTGCG1367926.601454630313874No Hit
CATAGAGAGATTTTGGGCTGAGACGATAATGCCGCATATCTACAACTATC36107.0203414880790325No Hit
CATAGAGAGATTTTGGGCTGAGACGATGGGGTTTTCTAGATATACAACAA19473.786317140523511No Hit
CATAGAACTTCACAAGGGCTTTAGGGGCTACACATGGCTATGCAATCTAT16303.169849480766987No Hit
CATAGACCTGGGCTTAGGGGCTACACATGGCTATGCAATCTATGAGACCA15993.1095639998444247No Hit
CATAGACCTGGGCTTAGGGGCTATACATGGCTATGCAATCTATGAGACCA15433.000661195597215No Hit
CATAGACACCATGCCTGGCTAATTTTTGTATTTTTAGTAGAGATAGGGTT14762.8703667690871613No Hit
CATAGACACCATGCCTGGCTAATTTTTTGCGTTTTTAGTAGAGATGGGGT12312.39391700050562No Hit
CATAGATCCCATCCAATAATAATGAGCAAGCAATGGGCAAGCAATGACAG10812.102213060557738No Hit
CATAGAGAGATTTTGGGCTGAGACGATGGGGTTTTCTAGATATACACTCA7741.5051923301310723No Hit
CATAGATCCCATCCAATAATAATGAGCAAGCAATGGGGAAAGGATTCTAT7311.4215705340126794No Hit
CATAGAGCCAAGGAAAGTTGAATGGAGACAGCCGCTCACCTCTATGAGAC6351.2348800124460348No Hit
CATAGAGAGATTTTGGGCTGAGACGATGGGGTTTTCTAGATATACAATCA5971.1609816809925713No Hit
CATAGACAATTAAATTTTCTTAAGGGGTTGCACAGATCATTTAAGCTCAA4890.9509548442300961No Hit
CATAGACCTGGGCTTAGGGGCTATACGTGAATGTGGATCAGAACTTCTAT4380.8517755046478162No Hit
CATAGAAAACAGAGTCACAGTCCCCAGTACACTGGAGACAGCCGCTCACC4080.7934347166582397No Hit
CATAGATCCTCTCATTGTCCAGTTTGGAGACAGCCGCTCACCTCTATGAG3650.7098129205398467No Hit
CATAGATGTCAAGAGTCTAATTCCTGCTTCCTTCTGCTGGCTATTCTATG3170.6164676597565245No Hit
CATAGAAAACAGAGTCACAGTCCCCAGTATCCCCAGAAATGTTAGCATGT3140.6106335809575668No Hit
CATAGACACCATGCCTGGCTAATTTTAAAATTAGTACCTATAATCTCAGC2940.5717397222978492No Hit
CATAGACACCATGCCTGGCTAATTTTTTGTATTTTTAGTAGAGATGGGGT2910.5659056434988916No Hit
CATAGACAGGGAGGCGTATAAGTGATGACTGATACAGGAAGGCCTTTCTA2500.4861732332464704No Hit
CATAGACCTTTGGGTTCATATCATCTGGAGACAGCCGCTCACCTCTATGA2490.4842285403134845No Hit
CATAGATCCCATCCAATAATAATGAACAAGCAATGGGGAAAGGATTCTAT2440.4745050756485551No Hit
CATAGATGAAAGCCTGTTGAATTTATGAATCTGTGAGTTGCTGATGCATC2400.4667263039166115No Hit
CATAGACCAATAAAAGTTGATGGGATGAACCAAGAATAAATGGGGGCAAG2300.44727937458675276No Hit
CATAGATCCCATCCAATAATAATGAGCAATGGGCAAGCAATGACAGTCTA2240.4356112169888375No Hit
CATAGAGCTGGCTTCCTAACTTCCAAACATGGTTCTACACATAGAGCTGC2040.39671735832911986No Hit
CATAGATGCCCTTTTTGTGTACTTCCACACTGACGACATGGTTCTACACA2030.3947726653961339No Hit
CATAGACACCATGCCTGGCTAATTTTTTGTATTAATCCCAGCTACTCAGG1920.3733810431332893No Hit
CATAGATGTCCATCATGTGGAAGCTGATCTATCCAGGAATTTTATTTTAA1920.3733810431332893No Hit
CATAGACACCATGCCTGGCTAATTTTTAAAAAGTAGCTGGGCATGGTGGC1880.3656022714013457No Hit
CATAGACACCATGCCTGGCTAATTTTTAAATATTTTGTAGAGACCGGGTT1810.35198942087044455No Hit
CATAGAAAACAGAGTCACAGTCCCCAGTAATGTTAGCATGTCTATGAGAC1810.35198942087044455No Hit
CATAGATGTCCATCATGTGGAAGCTGGAGACAGCCGCTCACCTCTATGAG1800.35004472793745867No Hit
CATAGACAGGGAGGCGTATAAGTGATGACACTGACGACACTGATCTATCC1660.32281902687565633No Hit
CATAGACACCATGCCTGGCTAATTTTTGTATTTTTAGTAGAGATGGGGTT1660.32281902687565633No Hit
CATAGACAATTAAATTTTCTTAAGGGGTTGACACTGACTGATACAGGAAG1640.3189296410096846No Hit
CATAGATCATGGGACTTCAGCATGGCCAACATGGTGAAACCCCGTCTCTA1590.30920617634475517No Hit
CATAGATGTCAAGAGTCTAATTCCTGCTCTAGTCACACTGGATTTCCTTC1580.3072614834117693No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCGGATTTGGACCTGCG1560.3033720975457975No Hit
CATAGAAACCCCAAAGGCTATGTTCAAAATGGTCAAACAGGAGGTCTATG1520.295593325813854No Hit
CATAGACACCATGCCTGGCTAATTTTTGTATTTTTAGTGGTGCACATGCC1410.2742017035510093No Hit
CATAGACACCATGCCTGGCTAATTTTTGTATTTTTTGTAGAGACAGAGTT1400.2722570106180234No Hit
CATAGACCTTTGGGTTCATATCATCTGGCTACACATGGCTATGCAATCTA1340.2605888530201081No Hit
CATAGAACTTCACAAGGGCTTTAGGGCTACACATGGCTATGCAATCTATG1320.2566994671541364No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATCTGGACCTGCG1300.25281008128816457No Hit
CATAGATCATGGGACTTCAGCATGGCCAACATGGTGAAACCCCATCTCTA1270.24697600248920695No Hit
CATAGACACCATGCCTGGCTAATTTTGTATTTTTAGTAGAGACAGGGTTT1190.2314184590253199No Hit
CATAGAGCTGGCTTCCTAACTTCCAACAATGGGCAAGCAATGACAGTCTA1180.22947376609233403No Hit
CATAGACAATTAAATTTTCTTAAGGGGTTGATCCTTTCCCTCACTCTCTC1180.22947376609233403No Hit
CATAGATCATGGGACTTCAGCATGGCCAACATGGTGAACCTGTCTCTACT1140.22169499436039047No Hit
CATAGAAAACAGAGTCACAGTCCCCAGTAATGTACCATGTTTTCATCCTG1130.21975030142740462No Hit
CATAGATGTATACAAGCTGTCACAGACAACACACTGACGACATGGTTCTA1110.21586091556143283No Hit
CATAGAAACCCCAAAGGCTATGTTCACACTGACGACATGGTTCTACACAT1060.20613745089650345No Hit
CATAGATCCCATCCAATAATAATGAGCAAGCAATGACAATGGGCAAGCAA1050.20419275796351755No Hit
CATAGATGAAAGCCTGTTGAATTTATGAGACCAAGAATCTGTGAGTTGCT1020.19835867916455993No Hit
CATAGATCCCATCCAATAATAATGAGCAAGCAATGACAGTCTATGAGACC980.19057990743261638No Hit
CATAGATGAAAGCCTGTTGAATTTATGAGACCAAGTCTCTGCTACCGTAG960.18669052156664465No Hit
CATAGATGTATACAAGCTGTCACAGACAACAAGCAATGGGGAAAGGATTC900.17502236396872933No Hit
CATAGATGTCCATCATGTGGAAGCTGACTGATACAGGAAGGCCTTTCTAT880.17113297810275757No Hit
CATAGATCCTCTCATTGTCCAGTTTGGCTACACATGGCTATGCAATCTAT850.16529889930379993No Hit
CATAGAGAGATTTTGGGCTGAGACGATGGCGTATTCTAAATATACAATCA840.16335420637081405No Hit
CATAGATCATGGGACTTCAGCATGGCCAACATGGTGAAACCCTGTCTCTA810.1575201275718564No Hit
CATAGATGTCAAGAGTCTAATTCCTGCTCTATGTGTAGCAGAATTACCTC810.1575201275718564No Hit
CATAGAGGATCCCATCTGAGTTGGTCTAAAGTGCAGTTTAAATTCAGTGC800.15557543463887052No Hit
CATAGATCATGGGACTTCAGCATGGAGACAGCCGCTCACCTCTATGAGAC790.15363074170588464No Hit
CATAGAACTTCACAAGGGCTTTAGGGGCTATACGTGAATGTGGATCAGAA760.147796662906927No Hit
CATAGACACCATGCCTGGCTAATTTTTGTATTTTTAGTAGTGGTGTGGTG760.147796662906927No Hit
CATAGACACCATGCCTGGCTAATTTTTGTATTTTTAGTAGAGACAGGGTT700.1361285053090117No Hit
CATAGAGAGATTTTGGGCTGAGACGATGGGGTTCTCTAAATATACACTCA700.1361285053090117No Hit
CATAGATGTCCATCATGTGGAAGCTGCCTTCCTAACCATGGGCATATCTA700.1361285053090117No Hit
CATAGAAAACAGAGTCACAGTCCCCAGTAATGTTAGCATGTCTATATCCC670.13029442651005405No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCCGCG630.12251565477811055No Hit
CATAGATCATGGGACTTCAGCATGGCAACCAACATGGCAAAACCCTGTCC600.11668157597915288No Hit
CATAGATTTGTAAATTGCCTAGTCTTGCATACACTGACTGATACAGGAAG600.11668157597915288No Hit
CATAGATGAAAGCCTGTTGAATTTATGAGACCAAGTCACCTGATTGGCCA590.11473688304616701No Hit
CATAGATCCCATCCAATAATAATGAGCAAGCAGGCAAGCAATGACAGTCT590.11473688304616701No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTCTGCAGATTTGGACCTGCG540.1050134183812376No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTCGCAGATTTGGACCTGCG530.10306872544825173No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)