FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005804528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005804528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173839
Sequences flagged as poor quality0
Sequence length150
%GC36

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGAGCTTGGGTGCGAACATCAAGGAAGTTTCTGTGAGATCTATGAGA172239.907443093897227No Hit
CATAGAGCTTTTTCTCCAGCCTCCAAGTAATAACCAGGCCCCTCTATGAG92645.32906885106334No Hit
CATAGACGGATTATTCTCTCCATGGTCTACCAGTTATTAGTTGAATGAGC87205.016135619740105No Hit
CATAGAGAAGACTCCCTCAACCCACCCACAGCCAGGAAAGAATCTATGAG77864.478856873313814No Hit
CATAGATCATGGGACTTCAGCATGGCTCAGGTATGTCTTTATTAGCTCTA57523.3088087253148024No Hit
CATAGAAATGATGCTGAAGGGCCTGCCCTCTCTGCTGTTCCTTCTATGAG54023.1074730066325738No Hit
CATAGACTCATTGCACTTTCCACAACTGTGTGCTTGAACCAGATTCTATG50392.8986591041135763No Hit
CATAGAGGAGGTGAAAGAAATCTACAATGGGTTCTGGGCCCCAGTGTGAA49572.851489021450883No Hit
CATAGATGTTTTATCAAAGGCATGCTCATCAGGAACCATGGGAGGTCTAT43942.5276261368277546No Hit
CATAGATCACGTAGAAACTGGAGAGGCTAATCTGAAATCTTCGTGTTCTA39432.2681906821829396No Hit
CATAGATGTTTTATCAAAGGCATGCTCATCAAGGAAGTTTCTGTGAGATC31941.8373322442029696No Hit
CATAGAAGTCACGAGTTTTGTTGTTGTTCTTCCTTTTCTGCCTTCATCTA30971.781533487882466No Hit
CATAGACTCATTGCACTTTCCACAACTGTCATTGTCGCTGTCCCATCTAT28221.6233411374892859No Hit
CATAGATGTTTTATCAAAGGCATGCTCATTTATTTTAAAGGACTGGAAGC27921.6060837901736666No Hit
CATAGACGTGACTAAAAGCATGTTGGTGGAAGGGTAAGAGAGGGTCTATG27401.5761710548265924No Hit
CATAGAAATGATGCTGAAGGGCCTGTTTCCCCACTCAAATACCATCTATG25041.4404132559437182No Hit
CATAGACCAGCAGACACTCCCATCATTTATTTTAAAGGACTGGAAGCCTC21001.2080143120933737No Hit
CATAGACTCATTGCACTTTCCACAACTGTCCCAACTGTCTCTGAAAAATT18531.065928819194772No Hit
CATAGAGGAGGTGAAAGAAATCTACAATGGGCCCCAGTGTGAATACTTCT16600.9549065514642858No Hit
CATAGACCAGCAGACACTCCCATCATCAGGAACCATGGGAGGTCTATGAG16320.9387996939697076No Hit
CATAGAAGGACAAAGACATTTAGGACCCTGCAGTCTTTGGAGGAATCTAT15530.8933553460385759No Hit
CATAGATGGCCAAGATACGAGCACATACACTGACGCTATACCACACTACA14540.8364060998970312No Hit
CATAGAAATGATGCTGAAGGGCCTGCTGAAGTGATGGGCTAGATCTATGA14300.8226002220445354No Hit
CATAGAAGCTGAGAAGACTTCCTAATGCAGAAGTAGGAAGTATTACCCCT14130.8128210585656843No Hit
CATAGAGAAGACTCCCTCAACCCACGCTGTCCTTCTCTGAATCTATGAGA13940.801891405265792No Hit
CATAGACACAACAGAGAGAACGGGCTACACTGACGCTATACCACACTACA13890.7990151807131887No Hit
CATAGACCAGCAGACACTCCCATCATCAAGGAAGTTTCTGTGAGATCTAT11900.6845414435195785No Hit
CATAGATCAAGGAAAACACCAGCCCACGCTGTCCTTCTCTGAATCTATGA10700.6155120542571No Hit
CATAGAGTCCAACTATTCCAGCACCACGCTGTCCTTCTCTGAATCTATGA10620.6109100949729347No Hit
CATAGACCAACATTTCTTCTCCCAGGGTACACTGACGCTATACCACACTA10180.5855993189100259No Hit
CATAGATGTGTAGCCTGGGTCTGACATGCCAGGAATCAATGTTCTATGAG9560.5499341344577454No Hit
CATAGAAGGACAAAGACATTTAGGACCCACGCTGTCCTTCTCTGAATCTA9180.5280748278579606No Hit
CATAGAAGCCATGAACACAGGATTGAGGAGCTGAGTCATGGGGTCTATGA8890.5113927254528616No Hit
CATAGACCTCCGACAGTTTTTATGCACCTTCATTGTTGCTCTATGAGACC8410.48378096974787016No Hit
CATAGAGTCTGAATGGTGGGAGAGTGAAAGAAATCTACAAAGGATTCTGG8110.46652362243225054No Hit
CATAGAGTGCCTGTTTCCTCATTCGTCAGTGGGACATGCCAGGAATCAAT7990.45962068350600266No Hit
CATAGATCAAGGAAAACACCAGCCCATGCAAACTGTTCTAGGCAGAAGAA7790.44811578529558965No Hit
CATAGAAAGGTGCAATCAAGCCAGTTACACTGACGCTATACCACACTACA7680.4417880912798624No Hit
CATAGAGCTTTTTCTCCAGCCTCCAAGGGGTAAGGCGTACTGGTCTATGA7600.43718613199569717No Hit
CATAGAAGTCACGAGTTTTGTTGTTGTTGCATGACAGAGCGAGACTTCTA7420.4268317236063254No Hit
CATAGACTCATTGCACTTTCCACAACTGTCTGCCTCTAAGTAAACTGAAT6940.39921996790133396No Hit
CATAGACCAGCAGACACTCCCATCATACACTGACGATCATTGTTCACTGC6460.3716082121963426No Hit
CATAGATCACGTAGAAACTGGAGAGGCCACTGACGATCATTGTTCACTGC6260.36010331398592954No Hit
CATAGACCAACATTTCTTCTCCCAGGGTACACTGTCATTGTCGCTGTCCC6030.34687268104395447No Hit
CATAGATCAAGGAAAACACCAGCCCACCCACAGCCAGGAAAGAATCTATG5620.32328763971260766No Hit
CATAGAGCTGTACTGCATCTTTTCTCAAACTGCTGGGGTTGCTCTATGAG5580.320986660070525No Hit
CATAGATGTGTAGCCTGGGTCTGACAGTGTGTCCCAACTGTCTCTGAAAA5550.31926092533896305No Hit
CATAGATCATGGGACTTCAGCATGGCCCTGCAGTCTTTGGAGGAATCTAT5220.3002778432917815No Hit
CATAGACGGATTATTCTCTCCATGGTCTGAGGAGAATTCAAGCTGCTCTA4970.2858967205287651No Hit
CATAGACACAACAGAGAGAACGGGCTATGCTGAAGTGATGGGCTAGATCT4940.2841709857972031No Hit
CATAGACCTCCGACAGTTTTTATGCACTGTCCCAACTGTCTCTGAAAAAT4900.28187000615512053No Hit
CATAGATGTTTTATCAAAGGCATGCTCATACACTGTCATTGTCGCTGTCC4900.28187000615512053No Hit
CATAGAGCTGTACTGCATCTTTTCTCAGACACACTGACGATCATTGTTCA4890.2812947612445999No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCTGCG4740.27266608758679006No Hit
CATAGACACAACAGAGAGAACGGGCTACCCAAGGGGTAAGGCGTACTGGT4590.2640374139289803No Hit
CATAGAGTAGTTAAAACCCACAATGACAGTGTATGAGGAGCTGAGTCATG4560.26231167919741827No Hit
CATAGAAATGATGCTGAAGTGATGGGCTAGATCTATGAGACCAAGTCTCT4450.25598398518169113No Hit
CATAGACCAACATTTCTTCTCCCAGGGTACACTGACGATCATTGTTCACT4330.24908104625544328No Hit
CATAGATGTTTTATCAAAGGCATGCTCATACACTGGCTCAGGTATGTCTT4300.24735531152388127No Hit
CATAGATCAAGGAAAACACCAGCCCAAATGATGCTGAAGTGATGGGCTAG4050.23297418876086495No Hit
CATAGAGTAGTTAAAACCCACAATGACAGTGTCAAGGGGTAAGGCGTACT3940.22664649474513776No Hit
CATAGACCAACATTTCTTCTCCCAGGGTACACTGACGACATGGCTCAGGT3910.2249207600135758No Hit
CATAGATGTTTTATCAAAGGCATGCTCATACACTGACGATCATTGTTCAC3870.22261978037149316No Hit
CATAGATCCACGTGAGAATAAAGCATACAACACTGACGATCATTGTTCAC3860.2220445354609725No Hit
CATAGAGTAGTTAAAACCCACAATGACAGTGTCATTGCTTCTTGTGGTAA3710.2134158618031627No Hit
CATAGAGTAGTTAAAACCCACAATGACAGTGTAGAGATCCCACCATTTGC3710.2134158618031627No Hit
CATAGAGTGCCTGTTTCCTCATTCGTACACTGACGCTATACCACACTACA3660.21053963725055944No Hit
CATAGAGCTGTACTGCATCTTTTCTCAGACCAGTGAAGCAGAGCTTCCCA3520.2024862085032703No Hit
CATAGAAATGATGCTGAAGGGCCTGCTGCTGAAGTGATGGGCTAGATCTA3440.19788424921910502No Hit
CATAGACCAACATTTCTTCTCCCAGGGTATAAAGCTGGACCCTCTCTGGG3430.19730900430858436No Hit
CATAGACGTGACTAAAAGCATGTTGGTCAGTACGCCTGCCCTCTCTGCTG3280.18868033065077455No Hit
CATAGAACAGATGTTCTCCTTGTTTTCTATAACAAGGGGTAAGGCGTACT3150.18120214681400607No Hit
CATAGACCAACATTTCTTCTCCCAGGGTCTACCAGTTATTAGTTGAATGA3130.18005165699296477No Hit
CATAGAGTAGTTAAAACCCACAATGACAGTGTACAAATGAGAAACCTGGA3070.17660018752984083No Hit
CATAGAAATGATGCTGAAGGGCCTGCATAGAAATGATGCTGAAGTGATGG2940.16912200369307231No Hit
CATAGACCCAAGACCCCAGCCTTTACCACTGCGACATGGTCTACCAGTTA2930.16854675878255168No Hit
CATAGAGTGCCTGTTTCCTCATTCGTCTGTTTCCCCACTCAAATACCATC2930.16854675878255168No Hit
CATAGAGTAGTTAAAACCCACAATGACAGTGGGGTTACCACCTTGCAAGA2920.16797151387203102No Hit
CATAGACTTGAAAGTGATAACTGAGAGGTAAACACACTGGCTCAGGTATG2880.1656705342299484No Hit
CATAGACTCATTGCACTTTCCACAACTGTCACTGACGCTATACCACACTA2880.1656705342299484No Hit
CATAGATGTTTTATCAAAGGCATGCTCATACACTGACGACATGGTTCTAC2670.15359039110901465No Hit
CATAGACTCATTGCACTTTCCACAACTGTACATAGAGATCCCACCATTTG2590.14898843182484944No Hit
CATAGAGTGCCTGTTTCCTCATTCGTGTCTGTTTCCCCACTCAAATACCA2550.1466874521827668No Hit
CATAGAAAAAACAGTTGGCCAGGCATGATGTTCTTCCTTTTCTGCCTTCA2520.14496171745120484No Hit
CATAGAAATGATGCTGAAGGGCCTGCTACCGTACAAGGGGTAAGGCGTAC2510.1443864725406842No Hit
CATAGATGTTTTATCAAAGGCATGCTCATCACTGACGCTATACCACACTA2500.14381122763016355No Hit
CATAGAGTCTGAATGGTGGGAGAGTGATAGGCTTAGAGGGCTTGCTCTAT2470.14208549289860156No Hit
CATAGAGCTGTACTGCATCTTTTCTCAGACTGCTGGGGTTGCTCTATGAG2360.1357577988828744No Hit
CATAGAAATGATGCTGAAGGGCCTGGATGCTGAAGTGATGGGCTAGATCT2220.12770437013558525No Hit
CATAGACCCAAGACCCCAGCCTTTACACTGACGACATGGTTCTACACATA2190.12597863540402326No Hit
CATAGAGTGCCTGTTTCCTCATTCGTCAGTGTTCCAAGTAATAACCAGGC2130.12252716594089934No Hit
CATAGATGTTTTATCAAAGGCATGCTCATGGTTCTACAAAGGATTCTGGG2100.12080143120933738No Hit
CATAGACCTCCGACAGTTTTTATGCAGAAGTAGGAAGTATTACCCCTCTA2080.11965094138829606No Hit
CATAGAAATGATGCTGAAGGGCCTGCAGAAGTAGGAAGTATTACCCCTCT1990.11447373719361018No Hit
CATAGAGATCCCACCATTTGCCATCTATGAGACCAAGTCTCTGCTACCGT1970.11332324737256888No Hit
CATAGACCCAGATGCTGTCCCACTAACTATAAGCCATAGGACAATGAATC1930.11102226773048625No Hit
CATAGAAATGATGCTGAAGGGCCTGCACACTGGCTCAGGTATGTCTTTAT1930.11102226773048625No Hit
CATAGAGTCTGAATGGTGGGAGAGTGCTGAAGTGATGGGCTAGATCTATG1920.1104470228199656No Hit
CATAGAACAGATGTTCTCCTTGTTTTCTATAACACACTGACGCTATACCA1920.1104470228199656No Hit
CATAGAAATGATGCTGAAGGGCCTGCTGGAGGCTAATCTGAAATCTTCGT1790.10296883898319709No Hit
CATAGACTCATTGCACTTTCCACAACTGCCCTCTCTGCTGTTCCTTCTAT1770.10181834916215579No Hit
CATAGACTCATTGCACTTTCCACAACTGTACTCATTGCTTCTTGTGGTAA1760.10124310425163514No Hit
CATAGAAATGATGCTGAAGGGCCTGCGATGCTGAAGTGATGGGCTAGATC1740.10009261443059382No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)