FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005804562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005804562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60591
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCTGCG2890347.70180389826872No Hit
CATAGAGAATTTGCACACCACGGGACATGGATGAAGCTGGATCTATGAGA20993.4642108563978145No Hit
CATAGATGTCTCCCAGGCTAGAGTGAGCCCTTGTCCCTTTCTATGAGACC12812.1141753725800863No Hit
CATAGACCACACCCAAACACCACGTACACTGACGACATGGTTCTACACAT10731.770890066181446No Hit
CATAGATCATGGGACTTCAGCATGGTGGGTGCCTGATTGTTCTATGAGAC7711.2724662078526514No Hit
CATAGAGAATTTGCACACCACGGGACCATGCTCACACCTTGATCTATGAG7221.191596111633741No Hit
CATAGAACCCAAAGAGTGAGCAGCAGAATCAGGGTTGGTTCTATGAGACC7111.173441600237659No Hit
CATAGACTTGATGCCCAGGAAACCTCCTGCCCCTGCAGGCTGAAAGTGCC6711.1074251951609975No Hit
CATAGAACAGACACAAATCTCAAAGCTTCTACACTGACGACATGGGCAAC5700.940733772342427No Hit
CATAGAGAAGAGAAGGCGACAATGGCATGAACTTGGGAGGCGGAGCTTGT5000.8252050634582694No Hit
CATAGAGGGTGTTTTTCGGTGTTACAACCAATGCTCTTTCATCTATGAGA4860.8020993216814378No Hit
CATAGAGCTTCTTTCTGTTCATATTGGGCAACAGAACAACAACATCTATG4160.68657061279728No Hit
CATAGACCTTCCTGTTGGCCACACAGAATTAGCGGAGAGTGAGAACTCTA4100.6766681520357809No Hit
CATAGAACCCAAAGAGTGAGCAGCAGCACTGACGACATGGTTCTACACAT3940.6502615900051163No Hit
CATAGAGCTTCTTTCTGTTCATATTGGGGAACAGAACAACAACATCTATG3390.5594890330247067No Hit
CATAGACACTGATAGGTTCCAACTTTCCTGGGAGAGGCTGTTTGAGATCT3290.5429849317555412No Hit
CATAGAGGGTGTTTTTCGGTGTCACAACCAATGCTCTTTCATCTATGAGA3230.533082470994042No Hit
CATAGAACAGACACAAATCTCAAAGCTTCTACACTGATCTGAGCAAGGAT3170.5231800102325428No Hit
CATAGAGGGTGTTTTTCGGTGTCACACACTGACGACATGGTTCTACACAT2500.4126025317291347No Hit
CATAGACAGCTCACAGACTGCATACTACACTGACGACATGGTGGGTGCCT2500.4126025317291347No Hit
CATAGAATGCAGGAGAATATACACAGGTTACACTGACGACATGGTGGGTG2260.3729926886831378No Hit
CATAGAACTATGTCAAGAGAATTTTGCACACCACGGGACATGGATGAAGC2130.35153735703322275No Hit
CATAGAGCTATCAGTATACTCCCACCATGATCTGAGCAAGGATAGCCCTC1800.29707382284497696No Hit
CATAGAACCCAAAGAGTGAGCAGCAGAGTGAGCCCTTGTCCCTTTCTATG1780.2937730025911439No Hit
CATAGATCTTTGGTAGATATGGGGTCTCCCAGGCCTGAGATTGCTGCTAT1660.2739680810681454No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTCGCAGATTTGGACCTGCG1630.26901685068739584No Hit
CATAGACTTGATGCCCAGGAAACCTCTCCACAAAAAACTTAAAACTTAGC1580.2607648000528131No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCCGCG1480.24426069878364776No Hit
CATAGATCATGGGACTTCAGCATGGCGGCGTTTGCAGATTTGGACCTGCG1390.2294070076413989No Hit
CATAGACTTGATGCCCAGGAAACCTCACACTGACGACATGGTGGGTGCCT1360.22445577726064925No Hit
CATAGAACAAGGCCAGAATTACCCTGGGAGAGGCTGTTTGAGATCTATGA1350.22280536713373272No Hit
CATAGAACAATGTAATAAGCATCGGAGGACACTGACGACATGGTTCTACA1340.2211549570068162No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTCTGCAGATTTGGACCTGCG1300.21455331649915002No Hit
CATAGACAGGAGGAGGAAGAAGAGGAATTCTTGCCAGGGATCTATGAGAC1270.20960208611840045No Hit
CATAGAGAAGAGAAGGCGACAATGGCGTGAACCCGGGAGGCGGAGCTTGC1260.2079516759914839No Hit
CATAGATCTTTGGTAGATATGGGGTCTCCCAGCAGAATCAGGGTTGGTTC1190.19639880510306812No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCTGTG1150.18979716459540194No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGGCCTGCG1140.18814675446848542No Hit
CATAGATTCTCCCTTGTGCTTTTGGTACACTGACGACATGGTTCTACACA1140.18814675446848542No Hit
CATAGATTCTCCCTTGTGCTTTTGGTACACTGACGACATGGTGGGTGCCT1140.18814675446848542No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCGGATTTGGACCTGCG1130.1864963443415689No Hit
CATAGAATGCAGGAGAATATACACAGGTTACACTGACGACATGGTTCTAC1120.18484593421465234No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGCTTGCAGATTTGGACCTGCG1100.18154511396081927No Hit
CATAGAACTATGTCAAGAGAATTTTGCACACCACGGGACCATGCTCACAC1090.1798947038339027No Hit
CATAGAATCAAAATAGTACCCTTGCCTCTGGTAGCAGGCAGTCTCAAACG1090.1798947038339027No Hit
CATAGAACTATGTCAAGAGAATTTTGCACATATACACCATGGAATACTAT1060.17494347345315311No Hit
CATAGACAGGAGGAGGAAGAAGAGGATCAGAGGAGCCCAGGAGTTGGAGA1040.17164265319932No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATCTGGACCTGCG1030.1699922430724035No Hit
CATAGATCATGGGACTTCAGCATGGTGGTGTTTGCAGATTTGGACCTGCG1020.16834183294548696No Hit
CATAGAACTATGTCAAGAGAATTTTGCACACACTGACGACATGGTTCTAC1010.1666914228185704No Hit
CATAGACTTGATGCCCAGGAAACCTCTAAAAAGATGCTCTTCCTCATTCG990.16339060256473734No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGACTTGGACCTGCG970.16008978231090426No Hit
CATAGAACTATGTCAAGAGAATTTTGCACAGGGATCTATGGGACCAAGTC960.15843937218398774No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTCGGACCTGCG960.15843937218398774No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGGTTTGGACCTGCG950.15678896205707119No Hit
CATAGAGGGTGTTTTTCGGTGTCACAGAGGACCATGCTCACACCTTGATC920.15183773167632156No Hit
CATAGACAGGAGGAGGAAGAAGAGGAAGAAGAAGAGAGCCTCTGTCCCTG900.14853691142248848No Hit
CATAGACCCTTAGAGTTTCAGCCTCCGAATTAGCTGGGATTATAGGCATC900.14853691142248848No Hit
CATAGAGGGCCAGAAGTTTGTTTACCTGGGAGAGGCTGTTTGAGATCTAT870.14358568104173886No Hit
CATAGATGTCTCCCAGGCTAGAGTGCGCCCTTGTCCCTTTCTATGAGACC870.14358568104173886No Hit
CATAGAGCTTCTTTCTGTTCATATTGGGGAACACTGACGACATGGTTCTA860.14193527091482233No Hit
CATAGAACAAGGCCAGAATTACCCTGTGCTCATACAGAATTAGATCTAGC840.13863445066098926No Hit
CATAGAACAATGTAATAAGCATCGGAGGACCATGCTCACACCTTGATCTA840.13863445066098926No Hit
CATAGAACCCAAAGAGTGAGCAGCAGGTTCTACACATAGAACCCAAAGAG770.12708157977257348No Hit
CATAGAACCCAAAGAGTGAGCAGCAGCTGACGACATGGTTCTACACATAG710.11717911901107426No Hit
CATAGAGGGTGTTTTTCGGTGTCACATGGTGGGTGCCTGATTGTTCTATG700.11552870888415771No Hit
CATAGAACCCAAAGAGTGAGCAGCAGAGAGTGAGCCCTTGTCCCTTTCTA680.11222788863032462No Hit
CATAGAACAGACACAAATCTCAAAGCTTCTCTATGAGACCAAGTCTCTGC670.1105774785034081No Hit
CATAGAGGGCCAGAAGTTTGTTTACCTACACTGGGACATGGATGAAGCTG670.1105774785034081No Hit
CATAGAACCCAAAGAGTGAGCAGCAGACGACATGGTTCTACACATAGAAC660.10892706837649156No Hit
CATAGACAGCTCACAGACTGCATACTACACTGACGACATGGTTCTACACA650.10727665824957501No Hit
CATAGAGAAGAGAAGGCGACAATGGCGTGAACCCGAGAGGTGGAGCTTGC650.10727665824957501No Hit
CATAGAACAGACACAAATCTCAAAGCTTCTACACTGACGACATGGTTCTA650.10727665824957501No Hit
CGTAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCTGCG630.10397583799574195No Hit
CATAGAACCCAAAGAGTGAGCAGCAGTTCTACACATAGAACCCAAAGAGT620.1023254278688254No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGTACCTGCG620.1023254278688254No Hit
CATAGACAGGAGGAGGAAGAAGAGGAGGAGGAGAAGAGGCAAAGTCACTG610.10067501774190886No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGATCTGCG610.10067501774190886No Hit
CATAGATCATGGGACTTCAGCATGGCGCGGGATCCATTCCCAACCCTCTT610.10067501774190886No Hit
CATAGAACCCAAAGAGTGAGCAGCAGAACCCAAAGAGTGAGCCCTTGTCC610.10067501774190886No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)