FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005804633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005804633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110867
Sequences flagged as poor quality0
Sequence length150
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNTAGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA65725.9278234280714726No Hit
NNNNGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA64175.788016271749032No Hit
NNNAGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA63625.738407280795908No Hit
NATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA60055.41639983042745No Hit
NNTNGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA50924.59289058060559No Hit
NNNNGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA17191.5505064626985487No Hit
NNTAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA16201.4612102789829253No Hit
NNNAGNGGTGAGCGGNTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA15821.4269349761425851No Hit
NNNAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA15811.426032994488892No Hit
NNTAGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA15511.398973544878097No Hit
NNNAGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA15241.3746200402283817No Hit
NNNNGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA13861.2501465720187253No Hit
NATAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA12881.161752369956795No Hit
NNTNGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA11151.0057095438678778No Hit
NATAGAGGTGAGCGGNTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA10140.9146093968448682No Hit
NNNAGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT5710.5150315242587966No Hit
NNTAGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT5710.5150315242587966No Hit
NNNAGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT5570.5024037811070923No Hit
NNTAGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT5480.4942859462238538No Hit
NNTAGNCTGCCTCAGCCTCCTGAGTAGGTCAGGAGATTGAGACCATCCTG5390.4861681113406153No Hit
NNNNGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT5320.4798542397647632No Hit
NATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT5260.4744423498426042No Hit
NNNNGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT5080.45820668007612725No Hit
NNNAGNCTGCCTCAGCCTCCTGAGTAGGTCAGGAGATTGAGACCATCCTG4660.42032345062101434No Hit
NNTNGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT4580.4131075973914691No Hit
NATAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT4550.41040165243038956No Hit
NATAGACTGCCTCAGCCTCCTGAGTAGGTCAGGAGATTGAGACCATCCTG4530.4085976891230032No Hit
NNNNGNCTGCCTCAGCCTCCTGAGTAGGTCAGGAGATTGAGACCATCCTG4500.40589174416192375No Hit
NNTNGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT4330.39055805604913996No Hit
NNTAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA3830.3454589733644818No Hit
NNTNGNCTGCCTCAGCCTCCTGAGTAGGTCAGGAGATTGAGACCATCCTG3740.3373411384812433No Hit
NNNNGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA3690.33283123021277744No Hit
NNNAGNATCCTTTCCNCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA3610.3256153769832322No Hit
NNNAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA3570.3220074503684595No Hit
NNTAGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGTGCCAC2870.25886873460993803No Hit
NNNAGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGTGCCAC2870.25886873460993803No Hit
NNTAGNCCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG2800.25255486303408586No Hit
NNTAGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGAAAG2700.24353504649715424No Hit
NNNAGNCCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG2630.2372211749213021No Hit
NNNNGNCCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG2550.2300053216917568No Hit
NNNAGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGAAAG2520.2272993767306773No Hit
NNNNGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGTGCCAC2460.22188748680851833No Hit
NNTAGNTTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT2430.21918154184743882No Hit
NNTAGNTTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA2410.21737757854005246No Hit
NATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGTGCCAC2330.21016172531050717No Hit
NNNNGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGAAAG2270.2047498353883482No Hit
NATAGACCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG2240.2020438904272687No Hit
NNNNGNTTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT2220.2002399271198824No Hit
NNTNGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGTGCCAC2140.19302407389033707No Hit
NATAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGAAAG2060.18580822066079178No Hit
NNTNGNCCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG2050.1849062390070986No Hit
NNNAGNTTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT2010.18129831239232594No Hit
NNNAGNTTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA1990.1794943490849396No Hit
NATAGAATCCTTTCCNCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA1970.1776903857775533No Hit
NNNNGNTTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA1890.17047453254800796No Hit
NNNAGNTTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA1850.16686660593323532No Hit
NNTAGNTTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA1770.15965075270369No Hit
NNTNGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGAAAG1760.15874877104999685No Hit
NNTNGNTTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT1650.14882697285937205No Hit
NATAGATTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA1650.14882697285937205No Hit
NNTNGNTTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA1620.14612102789829254No Hit
NNNNGNTTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA1590.14341508293721306No Hit
NNNAGNCTGCCTCAGNCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT1580.14251310128351988No Hit
NNNNGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT1550.1398071563224404No Hit
NNNNGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT1540.13890517466874724No Hit
NATAGATTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT1530.13800319301505407No Hit
NNNNGACTGCCTCAGCCTCCTGAGTAGGTCAGGAGATTGAGACCATCCTG1490.1343952664002814No Hit
NATAGATTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA1440.1298853581318156No Hit
NNNAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT1380.12447346820965663No Hit
NNNAGACTGCCTCAGCCTCCTGAGTAGGTCAGGAGATTGAGACCATCCTG1380.12447346820965663No Hit
NNNAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT1360.12266950490227028No Hit
NNTNGNTTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA1340.12086554159488395No Hit
NNNAGNCTGCCTCAGNCTCCTGAGTAGGTCAGGAGATTGAGACCATCCTG1330.1199635599411908No Hit
NNTAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT1250.11274770671164547No Hit
NNTAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT1210.10913978009687282No Hit
NNNAGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGTGCACC1180.10643383513579334No Hit
NATAGACTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA1170.10553185348210017No Hit
NNTAGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCAACATGGTG1150.10372789017471386No Hit
NNNAGNATCCTTTCCNCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT1150.10372789017471386No Hit
NNNNGNCTGCCTCAGCCTCCTGAGTAGCTGGAATTACAGGTGCCCGCCAC1140.10282590852102068No Hit
NNNNGNCTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA1110.1001199635599412No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)