FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005804638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005804638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53949
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCTGCG2585347.92118482270292No Hit
CATAGACTCAAGCAATCCTCCCACCTCAGCCTCCCAAATAGCCAGGACTA14112.6154330942186137No Hit
CATAGAGGGCTGTTTTGAGTGTTTGCCGTGGAGGAAAGGAGGTCTATGAG14002.5950434669780718No Hit
CATAGACTCAAGCAATCCTCCCACCTCAGCCTCCCAAGTAGCCAGGACTA12952.400415206954716No Hit
CATAGAGCTTCTGATCACTTTCGCCCCACCCTTATGATCGTATCTATGAG9121.6904854584885727No Hit
CATAGAGGGCTGTTTTGAGTGTTTGCAGATTTGGACCTGCGAGCGGGTTC6891.277132106248494No Hit
CATAGAACCAGAGTAATGAGGGATGCTCAGGTCTCCAATGGTGTCTATGA6841.2678640938664294No Hit
CATAGAACTTGTAATCATTCCCTACTTCCCACACTCATGCAAAGCTGGTC6821.2641568889136037No Hit
CATAGAACTTTGTGCCCCCAGTGTCTCCCCTCTATGAGACCAAGTCTCTG6631.2289384418617584No Hit
CATAGAACCAGAGTAATGAGGGATGCTCTCTCCAGAACCCACCATCTATG4980.9230940332536285No Hit
CATAGATGTGGCTATGCAGACTGAGCAGAGTTGGGTGTGTCTATGAGACC4640.8600715490555895No Hit
CATAGAAGATGGTCTGCTACACTGGTACACTGACGACATGGTTCTACACA4220.7822202450462473No Hit
CATAGATCATGCAAAGAACACAGGCCACACTCATGCAAAGCTGGTCTATG2590.48008304139094327No Hit
CATAGAAGGAGGGGAAACTGAGGCCACACTGACGACATGGTTCTACACAT2540.4708150290088787No Hit
CATAGAACTTTGTGCCCCCAGTGTCTTAGGTAGAGGAGTTTCAAACATCT2300.4263285695749689No Hit
CATAGACTCAAGCAATCCTCCCACCTCAGCTTCCCTAAGTACCAGGATTA2220.4114997497636657No Hit
CATAGAACTTTGTGCCCCCAGTGTCTCCCCTCTATGAGACCAAGTCTCTT2120.39296372499953663No Hit
CATAGAACTTTGTGCCCCCAGTGTCTCCCCTCTATGAGACCAAGTTCTTA2010.3725740977589946No Hit
CATAGATGTGCCTCTCCAACATTCATTTCCCCTCTCTCTGAATAACTCTA1980.3670132903297559No Hit
CATAGAAGGAGGGGAAACTGAGGCCAAATGGCATTAAATGACTTGCCCAA1930.35774527794769134No Hit
CATAGAAGATCCCATAGCAGCTTTGCTACCGTACAGTTTTTCCAAATATA1920.3558916754712784No Hit
CATAGATGTTAGGTGCTCATTTTAAAACATCAGGGATTATCAGTAGTGCT1860.34477006061280097No Hit
CATAGATGTGGCTATGCAGACTGAGTAGAGTTGGGTGTGTCTATGAGACC1760.3262340358486719No Hit
CATAGATCATGCAAAGAACACAGGCAGATCATGAGGTCAGGTCTATGAGA1730.32067322841943313No Hit
CATAGAACTTTGTGCCCCCAGTGTCTCCCCTCTTCTTATAGCCAGTGTCT1430.2650651541270459No Hit
CATAGAAGGAGGGGAAACTGAGGCCAAGTAGTGCAGCTATATTGGAGGAA1420.263211551650633No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGACTTGGACCTGCG1360.2520899367921556No Hit
CATAGAAGGAGGGGAAACTGAGGCCAAGTCAGCATGTTGAGATGAATAAT1340.24838273183932974No Hit
CATAGAGGGCTGTTTTGAGTGTTTGCCGTGGAGGAAAGCAATCACCGCCA1330.24652912936291685No Hit
CATAGAACTTTGTGCCCCCAGTGTCTCCCCTCTATGAGACCAAGTCTTCT1320.24467552688650393No Hit
CATAGAACTTTGTGCCCCCAGTGTCTCCCCTTCTTATAGCCAGTGTCTCC1310.242821924410091No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCGGATTTGGACCTGCG1270.23540751450443936No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTCTGCAGATTTGGACCTGCG1230.22799310459878774No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTCGCAGATTTGGACCTGCG1230.22799310459878774No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGGCCTGCG1190.2205786946931361No Hit
CATAGAAGAATTGCTTGAATCCGGGAGGCAGAGGTTGCAGTGAGCTGAGA1170.21687148974031029No Hit
CATAGAGGGCTGTTTTGAGTGTTTGCCGTGGAGGGAAGGAAGGGCAAACT1160.21501788726389737No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCCGCG1160.21501788726389737No Hit
CATAGAACTTTGTGCCCCCAGTGTCTTCTTATAGCCAGTGTCTCCCCTCT1110.20574987488183286No Hit
CATAGAAGATCCCATAGCAGCTTTGCTACCGTAAGTTTTTCCAAATATAA1110.20574987488183286No Hit
CATAGAAGGAGGGGAAACTGAGGCCAGGAGGCCCAATTGGACAGTGGCAC1080.20018906745259413No Hit
CATAGAAGGAGGGGAAACTGAGGCCAGACGCCGTGGCTCACACCTGTAAT960.1779458377356392No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGCTTGCAGATTTGGACCTGCG960.1779458377356392No Hit
CATAGATCATGCAAAGAACACAGGCCAGGCATGGTGGCTCATTCCTGTAA950.17609223525922632No Hit
CATAGATCATGGGACTTCAGCATGGCGGCGTTTGCAGATTTGGACCTGCG950.17609223525922632No Hit
CATAGAAGAATTGCTTGAATCCGGGAGGTGGAGGTTGCAGTGAGCCAAGA940.1742386327828134No Hit
CATAGAAGGAGGGGAAACTGAGGCCAAGTAGGAAAGCAATCACCGCCAGT930.17238503030640048No Hit
CATAGAGGGCTGTTTTGAGTGTTTGCTACCGTAGACGGAGTGAGGGCAGT930.17238503030640048No Hit
CATAGAAGGAGGGGAAACTGAGGCCAGGAGAGGTAGGGCCCGGCAAGTTG900.16682422287716175No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGGTTTGGACCTGCG900.16682422287716175No Hit
CATAGAAGATCCCATAGCAGCTTTGCTACCGTAGTTTTTCCAAATATAAG900.16682422287716175No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCTGCA870.16126341544792303No Hit
CATAGAACTTTGTGCCCCCAGTGTCTCCCCTCTATTCTTATAGCCAGTGT870.16126341544792303No Hit
CATAGAAGGAGGGGAAACTGAGGCCAAGTCTCTGCGGAGGGAAGGAAGGG850.15755621049509722No Hit
CATAGAGGGCTGTTTTGAGTGTTTGCCATGTCTTCTGTGTTTGGTCTATG830.1538490055422714No Hit
CATAGAGCTTCTGATCACTTTCGCCCTCTCTCCAGAACCCACCATCTATG830.1538490055422714No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTCGGACCTGCG820.15199540306585851No Hit
CATAGAAGGAGGGGAAACTGAGGCCACTGCCAGGCCCACAGATACTGTGC790.14643459563661979No Hit
CATAGAAGGAGGGGAAACTGAGGCCAGGAGAGGTAGGGCCCAGCAAGTTG770.14272739068379395No Hit
CATAGATCACCTACACAGACAGCTTTCCTACGGTGTCAGAGATAGCACAG740.13716658325455522No Hit
CATAGAAGATGGTCTGCTACACTGGTAACTTGTAATCATTTCCCCTCTCT740.13716658325455522No Hit
CATAGATGTGGCTATGCAGACGGAGTGAGGGCAGTAAGTCTATGAGACCA730.1353129807781423No Hit
CATAGACCCTTCTCCATGCCACACTCATGCAAAGCTGGTCTATGAGACCA720.1334593783017294No Hit
CATAGAAGATCCCATAGCAGCTTTGCCGTGGAGGAAAGGAGGTCTATGAG680.1260449683960778No Hit
CATAGAAGGAGGGGAAACTGAGGCCAAGGAGGGAAGGAAGGGCAAACTCT670.12419136591966487No Hit
CATAGAAGGAGGGGAAACTGAGGCCAGTCAGGGTGGCTCACGCCTGTAAT650.12048416096683906No Hit
CATAGATCACCTACACAGACAGCTTTCCCATGGGTCCTTTTCACATCATC640.11863055849042614No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATCTGGACCTGCG640.11863055849042614No Hit
CATAGACCCTTCTCCATGCCACATCATGCAAAGCTGGTCTATGAGACCAA630.11677695601401324No Hit
CATAGAACACAGATCTGCTTGGAGGGAAGGAAGGGCAAACTCTATGAGAC630.11677695601401324No Hit
CATAGAGGAAGTGCCTACCTTAAATTGATGGTCTCATAGAGGAGCACCCT630.11677695601401324No Hit
CATAGATCATGGGACTTCAGCATGGCTCTACACATAGATCATGGGACTTC600.11121614858477451No Hit
CATAGATCATGGGACTTCAGCATGGCAGTGTTTGCAGATTTGGACCTGCG590.10936254610836159No Hit
CATAGACTCAAGCAATCCTCCCACCTCGTCCTCCCAAAGTGCTGGGATTA570.10565534115553579No Hit
CATAGATGTGGCTATGCAGACTGAGTGAGGGCAGTAAGTCTATGAGACCA570.10565534115553579No Hit
CATAGAAGGAGGGGAAACTGAGGCCAGAGAAGTAAGGTGACTCTCCCCAG570.10565534115553579No Hit
CATAGAAGGAGGGGAAACTGAGGCCAGGCGCGGTGGCTCATGCCTGTAAT570.10565534115553579No Hit
CATAGAAGATCCCATAGCAGCTTTGCCTAATAGTTTTTCCAAATATAAGA560.10380173867912289No Hit
CATAGATCATGGGACTTCAGCATGGTGGTGTTTGCAGATTTGGACCTGCG540.10009453372629706No Hit
CATAGAAGGAGGGGAAACTGAGGCCAGGCGCAGTGGCTCACACCTGTCAT540.10009453372629706No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)