FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005804640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005804640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66377
Sequences flagged as poor quality0
Sequence length150
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGAGCTTGGGTGCGAACATCAAGGAAGTTTCTGTGAGATCTATGAGA741611.172544706750832No Hit
CATAGAGCTTTTTCTCCAGCCTCCAAGTAATAACCAGGCCCCTCTATGAG57168.611416605149374No Hit
CATAGAGAAGACTCCCTCAACCCACCCACAGCCAGGAAAGAATCTATGAG52297.877728731337662No Hit
CATAGAAATGATGCTGAAGGGCCTGCCCTCTCTGCTGTTCCTTCTATGAG43236.512798107778297No Hit
CATAGACTCATTGCACTTTCCACAACTGTGTGCTTGAACCAGATTCTATG31144.691384063757025No Hit
CATAGAGGAGGTGAAAGAAATCTACAATGGGTTCTGGGCCCCAGTGTGAA19472.9332449493047292No Hit
CATAGACGGATTATTCTCTCCATGGTCTACCAGTTATTAGTTGAATGAGC17612.653027404070687No Hit
CATAGAAATGATGCTGAAGGGCCTGCTGAAGTGATGGGCTAGATCTATGA15702.3652771291260524No Hit
CATAGATCATGGGACTTCAGCATGGCTCAGGTATGTCTTTATTAGCTCTA15322.3080283833255497No Hit
CATAGATGTTTTATCAAAGGCATGCTCATCAAGGAAGTTTCTGTGAGATC14102.124229778387092No Hit
CATAGAAGTCACGAGTTTTGTTGTTGTTCTTCCTTTTCTGCCTTCATCTA14042.115190502734381No Hit
CATAGACTCATTGCACTTTCCACAACTGTCATTGTCGCTGTCCCATCTAT13892.0925923136026032No Hit
CATAGACCAGCAGACACTCCCATCATCAGGAACCATGGGAGGTCTATGAG10251.5442095906714675No Hit
CATAGACTCATTGCACTTTCCACAACTGTCTGCCTCTAAGTAAACTGAAT9251.393554996459617No Hit
CATAGATCAAGGAAAACACCAGCCCACGCTGTCCTTCTCTGAATCTATGA8001.2052367536948039No Hit
CATAGAGCTTTTTCTCCAGCCTCCAAGGGGTAAGGCGTACTGGTCTATGA7721.1630534673154858No Hit
CATAGATGTTTTATCAAAGGCATGCTCATCAGGAACCATGGGAGGTCTAT6931.0440363378881239No Hit
CATAGACCAGCAGACACTCCCATCATCAAGGAAGTTTCTGTGAGATCTAT6701.0093857812193983No Hit
CATAGACGTGACTAAAAGCATGTTGGTGGAAGGGTAAGAGAGGGTCTATG5940.8948882896183918No Hit
CATAGACTCATTGCACTTTCCACAACTGTCCCAACTGTCTCTGAAAAATT5660.8527050032390737No Hit
CATAGAGGAGGTGAAAGAAATCTACAATGGGCCCCAGTGTGAATACTTCT5630.8481853654127183No Hit
CATAGACCAACATTTCTTCTCCCAGGGTACACTGACGCTATACCACACTA5420.8165479006282297No Hit
CATAGATCACGTAGAAACTGGAGAGGCTAATCTGAAATCTTCGTGTTCTA5410.815041354686111No Hit
CATAGATCACGTAGAAACTGGAGAGGCCAGTGCAGTGGGGAGGCAGGCTG4660.7020504090272233No Hit
CATAGACCAGCAGACACTCCCATCATTTATTTTAAAGGACTGGAAGCCTC4600.6930111333745123No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCTGCG3580.5393434472784248No Hit
CATAGAGAAGACTCCCTCAACCCACGCTGTCCTTCTCTGAATCTATGAGA3100.4670292420567365No Hit
CATAGAGAAGACTCCCTCAACCCACCACACTGACGATCATTGTTCACTGC2940.4429245069828404No Hit
CATAGATGTTTTATCAAAGGCATGCTCATTTATTTTAAAGGACTGGAAGC2870.43237868538801094No Hit
CATAGAAGCTGAGAAGACTTCCTAATGCAGAAGTAGGAAGTATTACCCCT2810.4233394097352999No Hit
CATAGACCAGCAGACACTCCCATCATGCTAGGCTAATCTGAAATCTTCGT2470.3721168477032707No Hit
CATAGACCAACATTTCTTCTCCCAGGGTGGAAGGGTAAGAGAGGGTCTAT2430.36609066393479667No Hit
CATAGAGAAGACTCCCTCAACCCACCACGCTGTCCTTCTCTGAATCTATG2070.3118550100185305No Hit
CATAGACTCATTGCACTTTCCACAACTGTACACTGACGCTATACCACACT2000.30130918842370097No Hit
CATAGAAGGACAAAGACATTTAGGACCCTGCAGTCTTTGGAGGAATCTAT1950.2937764587131084No Hit
CATAGACCAGCAGACACTCCCATCATTCGGTGGAAGGGTAAGAGAGGGTC1940.29226991277098996No Hit
CATAGAGTCCAACTATTCCAGCACCAAGGGGTAAGGCGTACTGGTCTATG1810.27268481552344936No Hit
CATAGACCAACATTTCTTCTCCCAGGGTACACTGACGACATGGCTCAGGT1780.26816517769709386No Hit
CATAGATGTGTAGCCTGGGTCTGACAGTGTGCTGAAGTGATGGGCTAGAT1670.25159317233379036No Hit
CATAGACCCAGATGCTGTCCCACTAATCTACAAAGGATTCTGGGCCATCT1630.2455669885653163No Hit
CATAGACATTTAATCCTCACAATGACCCATTGCTTCTTGTGGTAACTGGT1560.23502116697048675No Hit
CATAGAAAAAACAGTTGGCCAGGCATCCAAGGGGTAAGGCGTACTGGTCT1560.23502116697048675No Hit
CATAGAAGGACAAAGACATTTAGGACCCACACTGACGACATGGGTCTACC1550.23351462102836826No Hit
CATAGATGGCCAAGATACGAGCACATATGCTGAAGTGATGGGCTAGATCT1540.23200807508624977No Hit
CATAGACCAGCAGACACTCCCATCATAATGATGCTGAAGTGATGGGCTAG1540.23200807508624977No Hit
CATAGAAAAAACAGTTGGCCAGGCATCAAGGGGTAAGGCGTACTGGTCTA1510.22748843725989426No Hit
CATAGAGTCTGAATGGTGGGAGAGTGAAAGAAATCTACAAAGGATTCTGG1500.2259818913177757No Hit
CATAGACCTAAAAGGCTATTGAAAGGAAGGATGGTGGAAGGGTAAGAGAG1490.22447534537565722No Hit
CATAGATGAGGACACCATAAAGACAACAAGGGGTAAGGCGTACTGGTCTA1420.2139295237808277No Hit
CATAGAGTGCCTGTTTCCTCATTCGTCCCAACTGTCTCTGAAAAATTTCT1410.21242297783870917No Hit
CATAGAAGGACAAAGACATTTAGGACCCAGGCATCTATGCCATGGAACCA1410.21242297783870917No Hit
CATAGACGTGACTAAAAGCATGTTGGTCAGTGTCTACAAAGGATTCTGGG1320.19886406435964266No Hit
CATAGACCAACATTTCTTCTCCCAGGGTACACTGTGTGCTTGAACCAGAT1310.19735751841752414No Hit
CATAGACTTGAAAGTGATAACTGAGAGGTAAACCACATAGAGTGTCTGTT1310.19735751841752414No Hit
CATAGAAGTCACGAGTTTTGTTGTTGTTGCATGACAGAGCGAGACTTCTA1290.19434442653328712No Hit
CATAGATGGCCAAGATACGAGCACATATTCCTGTCATTGTCGCTGTCCCA1280.19283788059116863No Hit
CATAGAGTGCCTGTTTCCTCATTCGTACACTGACGCTATACCACACTACA1270.19133133464905014No Hit
CATAGACGTGACTAAAAGCATGTTGGTCAGTGTCAAGGGGTAAGGCGTAC1220.18379860493845762No Hit
CATAGAAAAAACAGTTGGCCAGGCATGTCCAAGGGGTAAGGCGTACTGGT1220.18379860493845762No Hit
CATAGAAGGACAAAGACATTTAGGACCCAGGCTTGCATGACAGAGCGAGA1170.17626587522786508No Hit
CATAGAGCTGTACTGCATCTTTTCTCAGACACACTGCCCTCTCTGCTGTT1150.17325278334362806No Hit
CATAGATGTTTTATCAAAGGCATGCTCATATGGTTCTACACATAGAAATG1130.17023969145939105No Hit
CATAGACGTGACTAAAAGCATGTTGGTCAGTGTGGGCCCCAGTGTGAATA1120.16873314551727256No Hit
CATAGAAATGATGCTGAAGGGCCTGCTGCTGAAGTGATGGGCTAGATCTA1100.16572005363303552No Hit
CATAGATGGCCAAGATACGAGCACATACACTGACGCTATACCACACTACA1090.16421350769091703No Hit
CATAGATGTGTAGCCTGGGTCTGACAGTGTGGTGGAAGGGTAAGAGAGGG1070.16120041580668004No Hit
CATAGACACAACAGAGAGAACGGGCTCCGAGGTCAGGAAGGAACCTGCTC1050.158187323922443No Hit
CATAGATCAAGGAAAACACCAGCCCAAGGGGTAAGGCGTACTGGTCTATG1040.1566807779803245No Hit
CATAGAGCTGTACTGCATCTTTTCTCAGACCACATAGAAATGATGCTGAA1030.15517423203820602No Hit
CATAGAGTGCCTGTTTCCTCATTCGTCTGTTTCCCCACTCAAATACCATC990.149148048269732No Hit
CATAGAGCTTGGGTGCGAACATCAACAAATCTACAAAGGATTCTGGGCCA980.14764150232761347No Hit
CATAGACGTGACTAAAAGCATGTTGGTCAGTGTGGCTCAGGTATGTCTTT980.14764150232761347No Hit
CATAGAGTGCCTGTTTCCTCATTCGTCAGTGTCTGAGGAGAATTCAAGCT950.14312186450125797No Hit
CATAGATGTTTTATCAAAGGCATGCTCATACACTGGCTCAGGTATGTCTT940.14161531855913945No Hit
CATAGAAATGATGCTGAAGTGATGGGCTAGATCTATGAGACCAAGTCTCT930.14010877261702095No Hit
CATAGACAGTGCCTTTGTGCGTGTTAGGAGAGCTTCACAACCTTGAAAGG920.13860222667490246No Hit
CATAGATCAAGGAAAACACCAGCCCATCATAGACCTCCCAAGTATTAGAG890.13408258884854693No Hit
CATAGAGTGCCTGTTTCCTCATTCGTCAGTGTGGTGGAAGGGTAAGAGAG880.13257604290642844No Hit
CATAGATGTTTTATCAAAGGCATGCTCATTGCTTCTTGTGGTAACTGGTC880.13257604290642844No Hit
CATAGATGTGTAGCCTGGGTCTGACAAATGAGAAACCTGGATGCTTCTAT880.13257604290642844No Hit
CATAGATGTTTTATCAAAGGCATGCTCATACACTGACGCTATACCACACT870.13106949696430995No Hit
CATAGAAATGATGCTGAAGGGCCTGCACATAGAAATGATGCTGAAGTGAT870.13106949696430995No Hit
CATAGATGTTTTATCAAAGGCATGCTCATGTGTGGTCTACCAGTTATTAG860.12956295102219142No Hit
CATAGAGAAGACTCCCTCAACCCACCCAGTACGCCTTACCCCCTGCAGTC830.1250433131958359No Hit
CATAGACTCATTGCACTTTCCACAACTGTACACTGACGATCATTGTTCAC830.1250433131958359No Hit
CATAGAGCTTTTTCTCCAGCCTCCAACACTGACGACATGGTTCTACACAT820.1235367672537174No Hit
CATAGAAATGATGCTGAAGGGCCTGGTTCTACACATAGAAATGATGCTGA800.1205236753694804No Hit
CATAGAAGCTGAGAAGACTTCCTAATGCACACTGACGATCATTGTTCACT780.11751058348524338No Hit
CATAGAGTAGTTAAAACCCACAATGACAGTGTCAAGGGGTAAGGCGTACT770.11600403754312488No Hit
CATAGAAGGACAAAGACATTTAGGACCCGTCTCATAGATGCTGAAGTGAT770.11600403754312488No Hit
CATAGAAGGACAAAGACATTTAGGACCCCTGGGCCCCAGTGTGAATACTT750.11299094565888786No Hit
CATAGAAATGATGCTGAAGGGCCTGTGCTGAAGTGATGGGCTAGATCTAT740.11148439971676936No Hit
CATAGACGTGACTAAAAGCATGTTGGTACCTGACGATCATTGTTCACTGC730.10997785377465086No Hit
CATAGACTCATTGCACTTTCCACAACTGTTCTTCCTTTTCTGCCTTCATC720.10847130783253237No Hit
CATAGATGTGTAGCCTGGGTCTGACAGTGTGGTCTACCAGTTATTAGTTG720.10847130783253237No Hit
CATAGACCGCTTCCCAATTTTTGTAACACCCACAGCCAGGAAAGAATCTA710.10696476189041385No Hit
CATAGAGCTGTACTGCATCTTTTCTCAGACACTGACGACATGGTTCTACA690.10395167000617685No Hit
CATAGAAATGATGCTGAAGGGCCTGCGACATGGTTCTACACATAGAAATG690.10395167000617685No Hit
CATAGAAATGATGCTGAAGGGCCTGGACGACATGGTTCTACACATAGAAA680.10244512406405834No Hit
CATAGAGGAGGTGAAAGAAATCTACAATGGGTCTACCAGTTATTAGTTGA680.10244512406405834No Hit
CATAGAAAAAACAGTTGGCCAGGCATACAAATGAGAAACCTGGATGCTTC680.10244512406405834No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)