FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005806250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005806250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35772
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA390710.921950128592195No Hit
CATAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA360910.088896343508889No Hit
CATAGATATGCCCATGGTTAGGAAGGAAGGATCAATATTGTTAAAATGGG23396.538633568153863No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGGTCAGGAGATTGAGACCATCCTG12343.449625405344962No Hit
CATAGACCCTGGATTAGCATGAGGAACATGGCTTCCCATTTCAGACAAGG11223.136531365313653No Hit
CATAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGAAAG8772.4516381527451636No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT6521.8226545901822655No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGAATTACAGGTGCCCGCCAC6211.7359946326735993No Hit
CATAGATGGAGAGAGTGAGGGAAAGGAAATTATGGGAGCCCTGTGAAACG6091.7024488426702449No Hit
CATAGATGACTTGGGGGAAAATGGTAGAAACAGCCAGTTTTCCCTTCTAT4501.2579671251257967No Hit
CATAGATTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA3851.0762607626076262No Hit
CATAGATTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA3781.0566923851056693No Hit
CATAGATTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT3210.897349882589735No Hit
CATAGAAAGAGACAGAAGAGAAGGTCATTTAAAAAGGAAACAGAGATTGG2970.8302583025830259No Hit
CATAGATTGAGCTTAAATGATCTGTGCAAGGTTAAACAATATCCAAATCC2830.7911215475791121No Hit
CATAGAGGTGAGCGGCTGTCTCCATTCAACTTTCCTTGGCTCTATGTGTA2820.7883260650788325No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGACTGCAGGCGCATGCTTG2670.7463938275746393No Hit
CATAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT2640.7380073800738007No Hit
CATAGACTGTCATTGCTTGCCCATTGGGATTTAAAAAAAGGAAAAGAACA2550.7128480375712848No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTGCCTGCCAC2540.7100525550710053No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGACTACAGGTGCCTGCTAT2400.6709158000670915No Hit
CATAGAGGTGAGCGGCTGTCTCCATGCTGAAGTCCCATGATCTATGTGTA2100.587051325058705No Hit
CATAGAATCCTTTCCCCATTGCTTGTTTTTCCCAGGTTTGTCAAAGATCA2070.5786648775578664No Hit
CATAGAATCCTTTCCCCATTGCTTGTTTTTGTCAAATTTGTTAAAAGAGG2060.5758693950575869No Hit
CATAGAATCCTTTCCCCATTGCTTGCTCATTATTATTGGATGGGATCTAT2030.5674829475567483No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGACGACAGCCCGGCTACTG1720.4808229900480823No Hit
CATAGACTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA1670.46684557754668454No Hit
CATAGATTGCATAGCCATGTGTAGCCATGCTGAAGTCCCATGATCTATGT1590.4444817175444482No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCGCACATCAC1570.43889075254388904No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTATAGGTGACTGCCAC1470.4109359275410936No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGCACTACAGGCGCCTGCCAC1420.3969585150396958No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGTGCCAC1380.38577658503857765No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCAACATGGTG1270.35502627753550264No Hit
CATAGAAGTTCTGATCCACATTCACGTATGTGTATAAATTATTTTGGTGC1240.346639830034664No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACGCACCAC1230.3438443475343844No Hit
CATAGACATGCTAACATTTCTGGGGATTGTGACTCTGTTTTCTATGTGTA1220.3410488650341049No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGGCTACAGGCACGTGCCAC1110.31029855753102986No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTGCCAC1100.3075030750307503No Hit
CATAGAGGTGAGCGGCTGTCTCCATGTCGTCAGTGTGCCATGCTGAAGTC1070.2991166275299117No Hit
CATAGATGAAAAGTCAAGCCAGCACACTCTAGCAGATCACGAGGTGAGGA1050.2935256625293525No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCGCCTGCCCC1030.28793469752879347No Hit
CATAGACCCTTACCAATAACACAATCAACACATATTTTACGTGTTACATA1010.28234373252823436No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGACTACAGGCACGTGCTAC1000.27954825002795486No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCGGGCACCAC990.27675276752767525No Hit
CATAGAATAGCCAGCAGAAGGAAGCAGGAATTAGACTCTTGACATCTATG990.27675276752767525No Hit
CATAGACTGCCTCAGCCTCCTGAGTACCTGGGATTACAGGTGCCTGCCAC960.26836632002683664No Hit
CATAGAATCCTTTCCCCATTGCTTGTTTTTGTCAGGTTTGTCAAAGATCA960.26836632002683664No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGATGGGATTATAGGCATCCACCAC950.2655708375265571No Hit
CATAGACTGTCATTGCTTGCCCATTGCTTGTTTTTCTCAGGTTTGTCAAA910.25438890752543886No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGACTACAGGCGCATGCTGC870.24320697752432072No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACACACACCCACTAC850.23761601252376158No Hit
CATAGAATCCTTTCCCCATTGCTTGTATTTGTCAGGTTTGTTGAAGATCA840.23482053002348205No Hit
CATAGAATCCTTTCCCCATTGCTTGTTTTTGTCAGGTTTGTCAAAGATGA840.23482053002348205No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTGTGTGCCAC830.2320250475232025No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGACATGTGCCAC820.22922956502292297No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGTTGCCCACTAC800.22363860002236388No Hit
CATAGAGGTGAGCGGCTGTCTCCACGACCGGCTAATTTTTGTATTTTTAG770.21525215252152521No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTGTGCACCAC750.20966118752096613No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGACTACAGGCGCCCGCCAC750.20966118752096613No Hit
CATAGACTGCCTCAGCCTCCTGAGTAACTGGGATTACAGGCACATGCTAC740.20686570502068657No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGAGATTATAGGTACTAATTTT730.20407022252040705No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGTATTACAGGCGCCTGCCAC730.20407022252040705No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTGCCCACCAC720.20127474002012746No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCGCCTGCCAC670.18729732751872974No Hit
CATAGAAAGGCCTTCCTGTATCAGTCAGTGTGAGCAGGAATTAGACTCTT670.18729732751872974No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGAGATACAGGCGCGCCCCACC670.18729732751872974No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTAGAGATGCGTGCTGC650.18170636251817063No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGTTGGGATTACAGGCGCCCGCCAC650.18170636251817063No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGACGTGCACCAC640.1789108800178911No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCCCCCACTAC630.17611539751761154No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCCAGAACTACATGCGTGTGCCAC630.17611539751761154No Hit
CATAGACTGCCTCAGCCTCCTGAGTACCTGGGATTACATGTGCCCACCAC610.17052443251705246No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGTGGGGATTATAGGTACCCATCAT610.17052443251705246No Hit
CATAGAATCCTTTCCCCATTGCTTGTTCATTATTATTGGATGGGATCTAT600.16772895001677288No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGCATTACAGATGTGTACCAC590.16493346751649335No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCCACCAC560.15654702001565468No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTGCATGCCAC560.15654702001565468No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGACTACAGGTGCCTGCCAC550.15375153751537515No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTGCCCGCCAC540.15095605501509562No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGATATTACAGGCGCACACCAC540.15095605501509562No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGAATTACAGGTGCGCACCAC540.15095605501509562No Hit
CATAGAATCCTTTCCCCATTGCTTGTTTTTGTCAGGAAGTCAAATTGTCC530.14816057251481604No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTATAGGTGCCCACCAC530.14816057251481604No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGACTACACATGTGTGTCAC510.14256960751425696No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGACTACAGGCATGAGCCAC510.14256960751425696No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACTGGCGCCTGCCAC500.13977412501397743No Hit
CATAGAGGTGAGCGGCTGTCTCCACTAAAAATACAAAAATTAGCCAGGCA490.13697864251369785No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGGCTACAGGAGCATGCCAA480.13418316001341832No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCGCCCGCCAC480.13418316001341832No Hit
CATAGACCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG460.1285921950128592No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGCGATTACAGGCGCCTGCCAC460.1285921950128592No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTAGGATTACAGGTGCGTGCCAC450.12579671251257968No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAAGCATGTACCAC430.12020574751202058No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGACTATAGGTACGCACCAC420.11741026501174102No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGGACGTATCAC420.11741026501174102No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGACTACAGGTGTGCACCAC390.10902381751090237No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGGGTGTGCCAC390.10902381751090237No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGGGTACAGGCACGAGCCAC380.10622833501062283No Hit
CATAGAAGTTCTGATCCACATTCACGTATAGCCCCTAAGCCCAGGTCTAT380.10622833501062283No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATCACAGGTGCATGCCAC380.10622833501062283No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGAGATTGCAGGCGTGTGCCAC380.10622833501062283No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGTACCAC370.10343285251034329No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGAATTACAGGTGTGCACCAC360.10063737001006373No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)