FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005806311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005806311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27059
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGAACTTCACAAGGGCTTTAGGGGCTACACATGGCTATGCAATCTAT18436.811042536679109No Hit
CATAGACCTGGGCTTAGGGGCTATACATGGCTATGCAATCTATGAGACCA16175.97583059240918No Hit
CATAGACCTGGGCTTAGGGGCTACACATGGCTATGCAATCTATGAGACCA15935.8871355186814No Hit
CATAGATCCCATCCAATAATAATGAGCAAGCAATGGGCAAGCAATGACAG13685.055619202483462No Hit
CATAGATCCCATCCAATAATAATGAGCAAGCAATGGGGAAAGGATTCTAT12764.715621419860305No Hit
CATAGATGCCCTTTTTGTGTACTTCCACACTGACGACATGGTTCTACACA10123.7399756088547247No Hit
CATAGACAGGGAGGCGTATAAGTGATGACTGATACAGGAAGGCCTTTCTA9943.67345430355889No Hit
CATAGAGCCAAGGAAAGTTGAATGGAGACAGCCGCTCACCTCTATGAGAC7612.8123729627850254No Hit
CATAGACCTGGGCTTAGGGGCTATACGTGAATGTGGATCAGAACTTCTAT5902.1804205624745925No Hit
CATAGATCCCATCCAATAATAATGAACAAGCAATGGGGAAAGGATTCTAT4671.7258583096197198No Hit
CATAGATGAAAGCCTGTTGAATTTATGATCTATCCAGGAATTTTATTTTA4161.5373812779481875No Hit
CATAGACCTTTGGGTTCATATCATCTGGAGACAGCCGCTCACCTCTATGA3431.2676004286928564No Hit
CATAGATCCTCTCATTGTCCAGTTTGGAGACAGCCGCTCACCTCTATGAG3151.164122842677113No Hit
CATAGATGTCAAGAGTCTAATTCCTGCTTCCTTCTGCTGGCTATTCTATG3111.1493403303891496No Hit
CATAGAGGATCCCATCTGAGTTGGTCTATGTACAAGCAATGGGGAAAGGA2811.0384714882294246No Hit
CATAGAAAACAGAGTCACAGTCCCCAGTAATGTTAGCATGTCTATGAGAC2400.8869507372778004No Hit
CATAGATCCCATCCAATAATAATGAGCAATGGGCAAGCAATGACAGTCTA2320.8573857127018737No Hit
CATAGATCATGGGACTTCAGCATGGAGACAGCCGCTCACCTCTATGAGAC2290.8462988284859012No Hit
CATAGACAGGGAGGCGTATAAGTGATGTTCTACCATTTTCCCCCAAGTCA2150.7945600354780294No Hit
CATAGATGCCCTTTTTGTGTACTTCCACTGACGACATGGTTCTACACATA2130.7871687793340478No Hit
CATAGAAAACAGAGTCACAGTCCCCAGTATCCCCAGAAATGTTAGCATGT2010.7428212424701578No Hit
CATAGAAAACAGAGTCACAGTCCCCAGTAATCCTTTCCCTCACTCTCTCC1930.7132562178942311No Hit
CATAGATGCCCTTTTTGTGTACTTCCAATCCTTTCCCTCACTCTCTCCAT1820.6726043091023319No Hit
CATAGAACCTCTTTTGGGGCAAAGTTCACAAAGTCACGAAAGTGGGTCTA1810.6689086810303411No Hit
CATAGATGAAAGCCTGTTGAATTTATGAGTACTCAGGAGGCTGAGGCAGT1780.6578217968143686No Hit
CATAGAGCTGGCTTCCTAACTTCCAATGGGCAAGCAATGACAGTCTATGA1760.650430540670387No Hit
CATAGATCATGGGACTTCAGCATGGCTATTGGAGACAGCCGCTCACCTCT1730.6393436564544145No Hit
CATAGAAAACAGAGTCACAGTCCCCAGTATAGAATCCTTTCCCTCACTCT1690.6245611441664511No Hit
CATAGATGCCCTTTTTGTGTACTTCCATGTGCTTCTTTCAAAAGTCTGGT1540.5691267230865885No Hit
CATAGACAGAAACCACCCAGACAACCTTCCTAACCATGGGCATATCTATG1520.5617354669426069No Hit
CATAGAACTTCACAAGGGCTTTAGGGCTACACATGGCTATGCAATCTATG1520.5617354669426069No Hit
CATAGATTCTGAAGAAATCAGATAGAGAGAAACAAGCAATGGGGAAAGGA1450.5358660704386711No Hit
CATAGATCCCATCCAATAATAATGAGCACACTGACGACATGGTTCTACAC1380.5099966739347352No Hit
CATAGATGTCAAGAGTCTAATTCCTGCTCTATGAGACCAAGTCTCTGACA1330.49151853357478104No Hit
CATAGATGTCAAGAGTCTAATTCCTGCTCCTATGAGACCAAGTCTCACCT1190.4397797405669093No Hit
CATAGATCATGGGACTTCAGCATGGCTACACATGGCTATGCAATCTATGA1190.4397797405669093No Hit
CATAGAAACCCCAAAGGCTATGTTCAAAATGGTCAAACAGGAGGTCTATG1160.42869285635093685No Hit
CATAGACCTTTGGGTTCATATCATCTGGCTACACATGGCTATGCAATCTA1080.39912783177501016No Hit
CATAGAACTTCACAAGGGCTTTAGGGGCTATACGTGAATGTGGATCAGAA1060.39173657563102854No Hit
CATAGATCCCATCCAATAATAATGAGCACAAAGTCACGAAAGTGGGTCTA950.3510846668391293No Hit
CNTAGACCTGGGCTTAGGGGCTACACATGGCTATGCAATCTATGAGACCA930.34369341069514764No Hit
CATAGATGTCAAGAGTCTAATTCCTGCTCTGTTATTCAAAGGTTTTCTGC920.33999778262315683No Hit
CNTAGACCTGGGCTTAGGGGCTATACATGGCTATGCAATCTATGAGACCA920.33999778262315683No Hit
CATAGATGAAAGCCTGTTGAATTTATGACCTGATTGGCCATTTGAACTCT900.33260652647917516No Hit
CATAGATCCCATCCAATAATAATGAGCAGAATTACCTCTGGATGGTCTAT860.3178240141912118No Hit
CNTAGAACTTCACAAGGGCTTTAGGGGCTACACATGGCTATGCAATCTAT830.3067371299752393No Hit
CATAGATCCCATCCAATAATAATGAGCAAGCAATGACAGTCTATGAGACC770.28456336154329426No Hit
CNTAGATGCCCTTTTTGTGTACTTCCACACTGACGACATGGTTCTACACA660.24391145275139509No Hit
CNTAGATCCCATCCAATAATAATGAGCAAGCAATGGGGAAAGGATTCTAT600.2217376843194501No Hit
CATAGAAAACAGAGTCACAGTCCCCAGTAATGTTAGCATGTCTATGTATC580.21434642817546842No Hit
CNTAGATCCCATCCAATAATAATGAGCAAGCAATGGGCAAGCAATGACAG570.2106508001034776No Hit
CATAGATGCCCTTTTTGTGTACTTCCATAGATGCCCTTTTTGTGTGCTTC530.19586828781551427No Hit
CATAGATGCCCTTTTTGTGTGCTTCTTTCAAAAGTCTGGTCTATGAGACC500.18478140359954176No Hit
CATAGAAAACAGAGTCACAGTCCCCAGTGCTGGCTTGACTTTTCATCTAT490.18108577552755092No Hit
CNTAGACAGGGAGGCGTATAAGTGATGACTGATACAGGAAGGCCTTTCTA490.18108577552755092No Hit
CATAGATGTCCATCATGTGGAAGCTGGCTATTCTATGAGACCAATGCTTC480.1773901474555601No Hit
CATAGAGCCAAGGAAAGTTGAATGGACAAGCAATGGGGAAAGGATTCTAT470.17369451938356925No Hit
CATAGATCCTCTCATTGTCCAGTTTGGCTACACATGGCTATGCAATCTAT450.16630326323958758No Hit
CATAGATGTCAAGAGTCTAATTCCTGCTCTATGAGACCAAGTCTCTGCTA440.16260763516759674No Hit
CATAGATTGGAACTTTGCCAGCCTTACACTGACTGATACAGGAAGGCCTT430.1589120070956059No Hit
CATAGATGCCCTTTTTGTGTACTTCCACGACATGGTTCTACACATAGATG400.1478251228796334No Hit
CATAGAAAACAGAGTCACAGTCCCCAGTAATGATCCCCAGAAATGTTAGC400.1478251228796334No Hit
CATAGATGTCCATCATGTGGAAGCTGCCTTCCTAACCATGGGCATATCTA400.1478251228796334No Hit
CATAGATGTCCATCATGTGGAAGCTGTCACAGACAAGCAATGGGGAAAGG380.14043386673565172No Hit
CATAGACAGAAACCACCCAGACAACCATGGTTCTACACAAAGTCACGAAA380.14043386673565172No Hit
CATAGATCATGGGACTTCAGCATGGCCTTCCTAACCATGGGCATATCTAT360.13304261059167005No Hit
CNTAGAGCCAAGGAAAGTTGAATGGAGACAGCCGCTCACCTCTATGAGAC340.12565135444768838No Hit
CATAGATGCCCTTTTTGTGTACTTCCATGTGTGCTTCTTTCAAAAGTCTG330.12195572637569754No Hit
CATAGATGTCCATCATGTGGAAGCTGCTTCCTTCTGCTGGCTATTCTATG320.1182600983037067No Hit
CATAGATCCCATCCAATAATAATGAGCAAGCAATGACAATGGGCAAGCAA300.11086884215972505No Hit
CATAGATGCCCTTTTTGTGTACTTCCAATGCCCTTTTTGTGTGCTTCCTT290.10717321408773421No Hit
CNTAGACCTGGGCTTAGGGGCTATACGTGAATGTGGATCAGAACTTCTAT280.10347758601574338No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)