FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005806312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005806312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27059
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNTAGNTTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT3481.2860785690528105No Hit
NNTAGNTTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA3231.1936878672530395No Hit
NNNAGNTTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT3051.1271665619572047No Hit
NNNNGNTTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT2781.027384604013452No Hit
NNNAGNTTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA2751.0162977197974796No Hit
NATAGATTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA2580.9534720425736354No Hit
NNTAGNCCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG2550.9423851583576628No Hit
NNTAGNTTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA2510.9276026460696996No Hit
NNNAGNTTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA2470.9128201337817362No Hit
NNNNGNTTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA2460.9091245057097453No Hit
NNNNGNTTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA2440.9017332495657637No Hit
NNNAGNCTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA2370.8758638530618279No Hit
NATAGATTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT2350.8684725969178462No Hit
NNTNGNTTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT2310.8536900846298828No Hit
NNNNGNCCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG2300.849994456557892No Hit
NNNAGNATCCTTTCCCCATTGCTTGCTCATTATTATTGGATGGGATCTAT2260.8352119442699287No Hit
NNNNGNCTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA2230.8241250600539561No Hit
NNTNGNTTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA2200.8130381758379837No Hit
NNNAGNCCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG2190.8093425477659929No Hit
NATAGATTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA2170.8019512916220112No Hit
NNTNGNTTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA2170.8019512916220112No Hit
NNTAGNATCCTTTCCCCATTGCTTGCTCATTATTATTGGATGGGATCTAT2170.8019512916220112No Hit
NATAGACCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG2120.7834731512620571No Hit
NNTAGNCTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA2080.7686906389740937No Hit
NNNAGNAAGGCCTTCCTGTATCAGTCATCACTTATACGCCTCCCTGTCTA2080.7686906389740937No Hit
NNNNGNATCCTTTCCCCATTGCTTGCTCATTATTATTGGATGGGATCTAT2020.7465168705421487No Hit
NNTNGNCCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG1990.7354299863261762No Hit
NNTAGNAAGGCCTTCCTGTATCAGTCATCACTTATACGCCTCCCTGTCTA1940.716951845966222No Hit
NATAGACTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA1920.7095605898222404No Hit
NATAGAATCCTTTCCCCATTGCTTGCTCATTATTATTGGATGGGATCTAT1820.6726043091023319No Hit
NNTNGNATCCTTTCCCCATTGCTTGCTCATTATTATTGGATGGGATCTAT1640.6060830038064969No Hit
NNTNGNCTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA1620.5986917476625152No Hit
NNNNGNAAGGCCTTCCTGTATCAGTCATCACTTATACGCCTCCCTGTCTA1570.5802136073025611No Hit
NNTAGNGGTGAGCGGCTGTCTCCATTCAACTTTCCTTGGCTCTATGTGTA1520.5617354669426069No Hit
NATAGAAAGGCCTTCCTGTATCAGTCATCACTTATACGCCTCCCTGTCTA1440.5321704423666802No Hit
NNNAGNGGTGAGCGGCTGTCTCCATTCAACTTTCCTTGGCTCTATGTGTA1410.5210835581507077No Hit
NNNNGNGGTGAGCGGCTGTCTCCATTCAACTTTCCTTGGCTCTATGTGTA1260.4656491370708452No Hit
NNTNGNAAGGCCTTCCTGTATCAGTCATCACTTATACGCCTCCCTGTCTA1260.4656491370708452No Hit
NNTAGNAGTTCTGATCCACATTCACGTATAGCCCCTAAGCCCAGGTCTAT1140.4213016002069552No Hit
NNNNGNAGTTCTGATCCACATTCACGTATAGCCCCTAAGCCCAGGTCTAT1130.41760597213496436No Hit
NATAGAGGTGAGCGGCTGTCTCCATTCAACTTTCCTTGGCTCTATGTGTA1080.39912783177501016No Hit
NNNAGNAGTTCTGATCCACATTCACGTATAGCCCCTAAGCCCAGGTCTAT1040.38434531948704687No Hit
NNNNGATTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT990.36586717912709266No Hit
NNTNGNGGTGAGCGGCTGTCTCCATTCAACTTTCCTTGGCTCTATGTGTA920.33999778262315683No Hit
NNTAGATTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT880.3252152703351935No Hit
NNNAGATTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT880.3252152703351935No Hit
NNNNGATTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA850.31412838611922095No Hit
NNNAGNATCCTTTCCCCATTGCTTGTTCATTATTATTGGATGGGATCTAT840.31043275804723014No Hit
NNNAGATTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA820.30304150190324847No Hit
NNNAGNGGTGAGCGGCTGTCTCCAGATGATATGAACCCAAAGGTCTATGT810.2993458738312576No Hit
NNNAGNTTGCATAGCNATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT800.2956502457592668No Hit
NNTAGNATCCTTTCCCCATTGCTTGTTCATTATTATTGGATGGGATCTAT780.2882589896152851No Hit
NNTAGNTTTAAAATAAAATTCCTGGATAGATCATAAATTCAACAGGCTTT780.2882589896152851No Hit
NNNNGACTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA760.28086773347130345No Hit
NNNAGNTTGCATAGCNATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA760.28086773347130345No Hit
NATAGATTTAAAATAAAATTCCTGGATAGATCATAAATTCAACAGGCTTT740.2734764773273218No Hit
NNNNGATTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA740.2734764773273218No Hit
NNNAGNTTGCATAGCNATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA730.2697808492553309No Hit
NNTAGNGGTGAGCGGCTGTCTCCAAACTGGACAATGAGAGGATCTATGTG710.2623895931113493No Hit
NNNNGACCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG690.2549983369673676No Hit
NNNAGNTTTAAAATAAAATTCCTGGATAGATCATAAATTCAACAGGCTTT690.2549983369673676No Hit
NNNNGAATCCTTTCCCCATTGCTTGCTCATTATTATTGGATGGGATCTAT690.2549983369673676No Hit
NATAGAAGTTCTGATCCACATTCACGTATAGCCCCTAAGCCCAGGTCTAT680.25130270889537676No Hit
NNTNGNAGTTCTGATCCACATTCACGTATAGCCCCTAAGCCCAGGTCTAT660.24391145275139509No Hit
NNTAGATTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA660.24391145275139509No Hit
NNNAGACTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA650.24021582467940428No Hit
NNNNGNATCCTTTCCCCATTGCTTGTTCATTATTATTGGATGGGATCTAT650.24021582467940428No Hit
NNNNGNATAGCCAGCAGAAGGAAGCAGGAATTAGACTCTTGACATCTATG640.2365201966074134No Hit
NNTNGNATCCTTTCCCCATTGCTTGTTCATTATTATTGGATGGGATCTAT630.2328245685354226No Hit
NNNAGNCCAGACTTTNGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG620.22912894046343174No Hit
NNTAGNGGTGAGCGGCTGTCTCCATGCTGAAGTCCCATGATCTATGTGTA620.22912894046343174No Hit
NNNNGNTTTAAAATAAAATTCCTGGATAGATCATAAATTCAACAGGCTTT610.22543331239144093No Hit
NATAGAATCCTTTCCCCATTGCTTGTTCATTATTATTGGATGGGATCTAT590.21804205624745926No Hit
NNNNGNGGTGAGCGGCTGTCTCCAAACTGGACAATGAGAGGATCTATGTG580.21434642817546842No Hit
NNTAGNGGTGAGCGGCTGTCTCCAGATGATATGAACCCAAAGGTCTATGT580.21434642817546842No Hit
NNNAGNCTGTCATTGNTTGCCCATTGCTTGCTCATTATTATTGGATGGGA570.2106508001034776No Hit
NNTAGNATAGCCAGCAGAAGGAAGCAGGAATTAGACTCTTGACATCTATG570.2106508001034776No Hit
NNNAGAATCCTTTCCCCATTGCTTGCTCATTATTATTGGATGGGATCTAT560.20695517203148675No Hit
NNTAGATTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA550.20325954395949591No Hit
NNNAGACCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG540.19956391588750508No Hit
NNNAGAAAGGCCTTCCTGTATCAGTCATCACTTATACGCCTCCCTGTCTA530.19586828781551427No Hit
NNNAGNATCCTTTCCCCATTGCTTGTACATAGACCAACTCAGATGGGATC530.19586828781551427No Hit
NNNAGATTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA530.19586828781551427No Hit
NNTAGACTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA520.19217265974352343No Hit
NNTAGNTGGAGAGAGTGAGGGAAAGGATTACTGGGGACTGTGACTCTGTT520.19217265974352343No Hit
NNNNGNGGTGAGCGGCTGTCTCCAGATGATATGAACCCAAAGGTCTATGT520.19217265974352343No Hit
NNTNGNTTTAAAATAAAATTCCTGGATAGATCATAAATTCAACAGGCTTT510.1884770316715326No Hit
NNTNGNGGTGAGCGGCTGTCTCCAGATGATATGAACCCAAAGGTCTATGT510.1884770316715326No Hit
NNTAGNATCCTTTCCCCATTGCTTGTACATAGACCAACTCAGATGGGATC510.1884770316715326No Hit
NNTAGACCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG500.18478140359954176No Hit
NNNAGNGGTGAGCGGCTGTCTCCATGCTGAAGTCCCATGATCTATGTGTA500.18478140359954176No Hit
NNNAGNATCCTTTCCNCATTGCTTGCTCATTATTATTGGATGGGATCTAT500.18478140359954176No Hit
NNTAGNTGGAGAGAGTGAGGGAAAGGATTCTATACTGGGGACTGTGACTC490.18108577552755092No Hit
NNNAGNGGTGAGCGGCTGTCTCCAAACTGGACAATGAGAGGATCTATGTG490.18108577552755092No Hit
NNTAGAATCCTTTCCCCATTGCTTGCTCATTATTATTGGATGGGATCTAT470.17369451938356925No Hit
NATAGAGGTGAGCGGCTGTCTCCAGATGATATGAACCCAAAGGTCTATGT470.17369451938356925No Hit
NNTAGNCTGTCATTGCTTGCCCATTGCTCATTATTATTGGATGGGATCTA460.16999889131157842No Hit
NNTNGNATAGCCAGCAGAAGGAAGCAGGAATTAGACTCTTGACATCTATG460.16999889131157842No Hit
NNNAGNCTGTCATTGCTTGCCCATTGCTCATTATTATTGGATGGGATCTA450.16630326323958758No Hit
NNNAGNTGGAGAGAGTGAGGGAAAGGATTACTGGGGACTGTGACTCTGTT430.1589120070956059No Hit
NNNAGNAAGGCCTTCNTGTATCAGTCATCACTTATACGCCTCCCTGTCTA430.1589120070956059No Hit
NNNNGAAAGGCCTTCCTGTATCAGTCATCACTTATACGCCTCCCTGTCTA420.15521637902361507No Hit
NNNNGAGGTGAGCGGCTGTCTCCATTCAACTTTCCTTGGCTCTATGTGTA420.15521637902361507No Hit
NNNNGNATCCTTTCCCCATTGCTTGTACATAGACCAACTCAGATGGGATC410.15152075095162423No Hit
NATAGAATCCTTTCCCCATTGCTTGTACATAGACCAACTCAGATGGGATC410.15152075095162423No Hit
NNNAGNATAGCCAGCAGAAGGAAGCAGGAATTAGACTCTTGACATCTATG400.1478251228796334No Hit
NNNAGNTGGAGAGAGTGAGGGAAAGGATTGGAAGTACACAAAAAGGGCAT400.1478251228796334No Hit
NNTNGNTGGAGAGAGTGAGGGAAAGGATTGGAAGTACACAAAAAGGGCAT390.14412949480764256No Hit
NATAGATGGAGAGAGTGAGGGAAAGGATTACTGGGGACTGTGACTCTGTT390.14412949480764256No Hit
NNTAGNCATGCTAACATTTCTGGGGATACTGGGGACTGTGACTCTGTTTT390.14412949480764256No Hit
NNTAGNCCCACTTTCGTGACTTTGTGAACTTTGCCCCAAAAGAGGTTCTA390.14412949480764256No Hit
NNTAGNTGGAGAGAGTGAGGGAAAGGATTGGAAGTACACAAAAAGGGCAT390.14412949480764256No Hit
NATAGAGGTGAGCGGCTGTCTCCAAACTGGACAATGAGAGGATCTATGTG380.14043386673565172No Hit
NNNNGNTGGAGAGAGTGAGGGAAAGGATTACTGGGGACTGTGACTCTGTT380.14043386673565172No Hit
NATAGATTGCATAGCNATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA380.14043386673565172No Hit
NATAGATTGCATAGCNATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA380.14043386673565172No Hit
NATAGAGGTGAGCGGCTGTCTCCAATAGCCATGCTGAAGTCCCATGATCT380.14043386673565172No Hit
NNNAGNGGTGAGCGGNTGTCTCCATTCAACTTTCCTTGGCTCTATGTGTA370.1367382386636609No Hit
NATAGAATAGCCAGCAGAAGGAAGCAGGAATTAGACTCTTGACATCTATG370.1367382386636609No Hit
NNNNGNTGGAGAGAGTGAGGGAAAGGATTCTATACTGGGGACTGTGACTC360.13304261059167005No Hit
NNNNGNGGTGAGCGGCTGTCTCCATGCTGAAGTCCCATGATCTATGTGTA360.13304261059167005No Hit
NNTAGAAAGGCCTTCCTGTATCAGTCATCACTTATACGCCTCCCTGTCTA360.13304261059167005No Hit
NNTNGNATCCTTTCCCCATTGCTTGTACATAGACCAACTCAGATGGGATC360.13304261059167005No Hit
NNNAGNCTGCCTCAGCCTCCTGAGTACTCATAAATTCAACAGGCTTTCAT360.13304261059167005No Hit
NNNAGNTGGAGAGAGTGAGGGAAAGGATTCTATACTGGGGACTGTGACTC350.12934698251967922No Hit
NNNAGNTGACTTGGGGGAAAATGGTAGAACATCACTTATACGCCTCCCTG350.12934698251967922No Hit
NNNNGNTGGAGAGAGTGAGGGAAAGGATTGGAAGTACACAAAAAGGGCAT350.12934698251967922No Hit
NNNAGNCATGCTAACATTTCTGGGGATACTGGGGACTGTGACTCTGTTTT350.12934698251967922No Hit
NATAGATGGAGAGAGTGAGGGAAAGGATTGGAAGTACACAAAAAGGGCAT340.12565135444768838No Hit
NNTAGNGGTGAGCGGCTGTCTCCAATAGCCATGCTGAAGTCCCATGATCT340.12565135444768838No Hit
NNNAGNCTGTCATTGCTTGCCCATTGGAAGTTAGGAAGCCAGCTCTATGT340.12565135444768838No Hit
NATAGACATGCTAACATTTCTGGGGATACTGGGGACTGTGACTCTGTTTT340.12565135444768838No Hit
NNTNGNTGGAGAGAGTGAGGGAAAGGATTACTGGGGACTGTGACTCTGTT340.12565135444768838No Hit
NNTAGNCTGTCATTGCTTGCCCATTGGAAGTTAGGAAGCCAGCTCTATGT340.12565135444768838No Hit
NNTAGAGGTGAGCGGCTGTCTCCATTCAACTTTCCTTGGCTCTATGTGTA330.12195572637569754No Hit
NNTAGNTGACTTGGGGGAAAATGGTAGAACATCACTTATACGCCTCCCTG330.12195572637569754No Hit
NNNNGNATCCTTTCCCCATTGCTTGTTTCTCTCTATCTGATTTCTTCAGA330.12195572637569754No Hit
NNTNGNTGACTTGGGGGAAAATGGTAGAACATCACTTATACGCCTCCCTG310.11456447023171587No Hit
NNNNGNCTGCCTCAGCCTCCTGAGTACTCATAAATTCAACAGGCTTTCAT310.11456447023171587No Hit
NATAGACCAGACTTTNGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG310.11456447023171587No Hit
NNNAGNGGTGAGCGGCTGTCTCCAATAGCCATGCTGAAGTCCCATGATCT300.11086884215972505No Hit
NNNNGAAGTTCTGATCCACATTCACGTATAGCCCCTAAGCCCAGGTCTAT300.11086884215972505No Hit
NATAGATTGCATAGCNATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT300.11086884215972505No Hit
NNNNGNCTGTCATTGCTTGCCCATTGCTCATTATTATTGGATGGGATCTA300.11086884215972505No Hit
NNNNGNCCAGACTTTTGAAAGAAGCACATGGAAGTACACAAAAAGGGCAT300.11086884215972505No Hit
NNNNGNCATGCTAACATTTCTGGGGATACTGGGGACTGTGACTCTGTTTT300.11086884215972505No Hit
NNNNGNATCCTTTCCCCATTGCTTGTCAGAGACTTGGTCTCATAGAGCAG290.10717321408773421No Hit
NNNAGNAGTTCTGATNCACATTCACGTATAGCCCCTAAGCCCAGGTCTAT290.10717321408773421No Hit
NNTNGNCTGTCATTGCTTGCCCATTGCTCATTATTATTGGATGGGATCTA290.10717321408773421No Hit
NNTNGNGGTGAGCGGCTGTCTCCATGCTGAAGTCCCATGATCTATGTGTA290.10717321408773421No Hit
NNTAGNTATGCCCATGGTTAGGAAGGTTGTCTGGGTGGTTTCTGTCTATG280.10347758601574338No Hit
NNNNGNGGTGAGCGGCTGTCTCCAATAGCCATGCTGAAGTCCCATGATCT280.10347758601574338No Hit
NNNNGNTTGCATAGCCATGTGTAGCCCTAAAGCCCTTGTGAAGTTCTATG280.10347758601574338No Hit
NNTNGNGGTGAGCGGCTGTCTCCAAACTGGACAATGAGAGGATCTATGTG280.10347758601574338No Hit
NNTAGNCCAGACTTTTGAAAGAAGCACATGGAAGTACACAAAAAGGGCAT280.10347758601574338No Hit
NNNNGNCTGTCATTGCTTGCCCATTGGAAGTTAGGAAGCCAGCTCTATGT280.10347758601574338No Hit
NNNAGNCCCACTTTCGTGACTTTGTGAACTTTGCCCCAAAAGAGGTTCTA280.10347758601574338No Hit
NNTAGNATCCTTTCCCCATTGCTTGTTTCTCTCTATCTGATTTCTTCAGA280.10347758601574338No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)