FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005806348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005806348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32578
Sequences flagged as poor quality0
Sequence length150
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA598718.37743262324268No Hit
CATAGAGCTAATAAAGACATACCTGAGCCATGCTGAAGTCCCATGATCTA21116.479833016145865No Hit
CATAGATCTCACAGAAACTTCCTTGATGAGCATGCCTTTGATAAAACATC16965.205967217140402No Hit
CATAGAGGGGCCTGGTTATTACTTGGAGGCTGGAGAAAAAGCTCTATGTG16695.123089201301491No Hit
CATAGAGCTCATTCAACTAATAACTGGTAGACCATGGAGAGAATAATCCG16104.941985388912763No Hit
CATAGATTCTTTCCTGGCTGTGGGTGGGTTGAGGGAGTCTTCTCTATGTG11973.674258702191663No Hit
CATAGAAGGAACAGCAGAGAGGGCAGGCCCTTCAGCATCATTTCTATGTG11653.576032905641844No Hit
CATAGATGAAGGCAGAAAAGGAAGAACAACAACAAAACTCGTGACTTCTA10583.247590398428387No Hit
CATAGAATCTGGTTCAAGCACACAGTTGTGGAAAGTGCAATGAGTCTATG8012.458714469887654No Hit
CATAGACCTCCCATGGTTCCTGATGAGCATGCCTTTGATAAAACATCTAT7602.332862668058199No Hit
CATAGACCCTCTCTTACCCTTCCACCAACATGCTTTTAGTCACGTCTATG5571.7097427711952853No Hit
CATAGAGGTGAGCGGCTGTCTCCATGCTGAAGTCCCATGATCTATGTGTA4221.2953526920007368No Hit
CATAGATCTAGCCCATCACTTCAGCAGGCCCTTCAGCATCATTTCTATGT4011.2308920130149181No Hit
CATAGATGGGACAGCGACAATGACAGTTGTGGAAAGTGCAATGAGTCTAT3701.135735772607281No Hit
CATAGACCAGTACGCCTTACCCCTTGGAGGCTGGAGAAAAAGCTCTATGT3020.9270059549389158No Hit
CATAGAACACGAAGATTTCAGATTAGCCTCTCCAGTTTCTACGTGATCTA2690.8257106022469152No Hit
CATAGATCTCACAGAAACTTCCTTGATGATGGGAGTGTCTGCTGGTCTAT2580.7919454846829148No Hit
CATAGAAATTTTTCAGAGACAGTTGGGACAGTTGTGGAAAGTGCAATGAG2570.788875928540733No Hit
CATAGAGCTAATAAAGACATACCTGAGCCAGGTGCGATGGCTTTCTGACT2500.7673890355454601No Hit
CATAGATTCCTCCAAAGACTGCAGGGTCCTAAATGTCTTTGTCCTTCTAT2290.7029283565596415No Hit
CATAGATTCAGAGAAGGACAGCGTGGGTTGAGGGAGTCTTCTCTATGTGT2120.6507459021425502No Hit
CATAGATTCAGAGAAGGACAGCGTGGTCACATAGGTTGCTAGACAGATTT2020.6200503407207317No Hit
CATAGATTCAGTTTACTTAGAGGCAGACAGTTGTGGAAAGTGCAATGAGT1930.5924243354410952No Hit
CATAGACCCTCTCTTACCCTTCCACCCTGGGAGAAGAAATGTTGGTCTAT1650.5064767634600037No Hit
CATAGAGGTGAGCGGCTGTCTCCATGAATGATGGGAGTGTCTGCTGGTCT1570.481920314322549No Hit
CATAGAGGGGTAATACTTCCTACTTCTGCATTAGGAAGTCTTCTCAGCTT1520.46657253361163975No Hit
CATAGAGGCTTCCAGTCCTTTAAAATAAATGATGGGAGTGTCTGCTGGTC1510.4635029774694579No Hit
CATAGATGGTATTTGAGTGGGGAAACAGGCCCTTCAGCATCATTTCTATG1500.46043342132727605No Hit
CATAGAACATTGATTCCTGGCATGTCAGACCCAGGCTACACATCTATGTG1380.42359874762109395No Hit
CATAGAACAACTTGCTCTAATACTTGGGAGAGGAAATGATTTCCCTTGAA1360.41745963533673036No Hit
CATAGACCCCATGACTCAGCTCCTCACTCTCCCACCATTCAGACTCTATG1240.3806249616305482No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGATCA1200.36834673706182086No Hit
CATAGAAGTATTCACACTGGGGCCCATTGTAGATTTCTTTCACCTCCTCT1190.365277180919639No Hit
CATAGATTCAGAGAAGGACAGCGTGGTGCTGGAATAGTTGGACTCTATGT1150.35299895635091166No Hit
CATAGACCCCATGACTCAGCTCCTCATTCTAAAAAAGAACGATGAAATAC1070.3284425072134569No Hit
CATAGACCTCCCATGGTTCCTGATGATGGGAGTGTCTGCTGGTCTATGTG1040.3192338387869114No Hit
CATAGATGGTATTTGAGTGGGGAAACAGACACTCTATGTGTAGAACCATG1020.31309472650254777No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGGGCCTTCAGGTCA1010.31002517036036586No Hit
CATAGATGGCAAATGGTGGGATCTCTATGTGTAGAACCATGTCGTCAGTG960.29467738964945667No Hit
CATAGAGGTGAGCGGCTGTCTCCAGTGTGGGCTGGTGTTTTCCTTGATCT890.2731904966541838No Hit
CATAGAAGGAACAGCAGAGAGGGCAGTGGGGTCCTAAATGTCTTTGTCCT880.270120940512002No Hit
CATAGATGGCAAATGGTGGGATCTCTATATGATGGGAGTGTCTGCTGGTC840.25784271594327457No Hit
CATAGAGCAGCTTGAATTCTCCTCAGACCATGGAGAGAATAATCCGTCTA810.2486340475167291No Hit
CATAGACCAGTACGCCTTACCCCTTGGGCTGGTGTTTTCCTTGATCTATG800.24556449137454725No Hit
CATAGAGGTGAGCGGCTGTCTCCATAGATGCCTGGCCAACTGTTTTTTCT800.24556449137454725No Hit
CATAGATGGTATTTGAGTGGGGAAACAGACACTCTATGGCCATGCTGAAG770.2363558229480017No Hit
CATAGACCCCATGACTCAGCTCCTCAGTGTTGGAATAGGCCATGCTGAAG690.21179937381054698No Hit
CATAGATCTAGCCCATCACTTCAGCATCATTTCTATGTGTAGAACCATGT690.21179937381054698No Hit
CATAGAACATTGATTCCTGGCATGTCCCATGATTTACCATCACAAATTTT680.20872981766836518No Hit
CATAGAGCAACCCCAGCAGTTTGAGAAAAGATGCAGTACAGCTCTATGTG660.20259070538400148No Hit
CATAGACCCTCTCTTACCCTTCCACCTTCCTTTCAATAGCCTTTTAGGTC640.1964515930996378No Hit
CATAGAAATTTTTCAGAGACAGTTGGGACACACTGACGAATGAGGAAACA630.19338203695745596No Hit
CATAGATGGGACAGCGACAATGACAGCAGCAGCTCTGTCTTGCAGCAACT630.19338203695745596No Hit
CATAGAGCAGCTTGAATTCTCCTCAGAAAATGGGATTTTCTTTTCTATTG620.1903124808152741No Hit
CATAGAACAACTTGCTCTAATACTTGGGAGGTCACGAATGAGGAAACAGG590.18110381238872858No Hit
CATAGAACACGAAGATTTCAGATTAGCCTGGTGCTGGAATAGTTGGACTC590.18110381238872858No Hit
CATAGACCAGTACGCCTTACCCCTTGCATTAGGAAGTCTTCTCAGCTTCT580.17803425624654676No Hit
CATAGATTCAGAGAAGGACAGCGTGGGCTGGTGTTTTCCTTGATCTATGT570.1749647001043649No Hit
CATAGAGGCTTCCAGTCCTTTAAAATAAATGACTGTTAATATAGAAGAGA560.17189514396218308No Hit
CATAGACCAGTACGCCTTACCCCTTGGACTTGGGTCCTAAATGTCTTTGT540.16575603167781938No Hit
CATAGATCTCACAGAAACTTCCTTGATGACTGTTAATATAGAAGAGACAC530.16268647553563756No Hit
CATAGAGGTGAGCGGCTGTCTCCATGTGGGTGGGTTGAGGGAGTCTTCTC530.16268647553563756No Hit
CATAGAGGCTTCCAGTCCTTTAAAATAAATGAGCATGCCTTTGATAAAAC520.1596169193934557No Hit
CATAGAGCAAGCCCTCTAAGCCTATCACTCTCCCACCATTCAGACTCTAT490.15040825096691018No Hit
CATAGATCTAGCCCATCACTTCAGCAGCAGGCCCTTCAGCATCATTTCTA490.15040825096691018No Hit
CATAGATGGTATTTGAGTGGGGAAACAGACGAATGAGGAAACAGGCACTC480.14733869482472833No Hit
CATAGATGGTATTTGAGTGGGGAAACAGACACTCGTTGATGTTCGCACCC470.14426913868254648No Hit
CATAGATGGTATTTGAGTGGGGAAACAGATCCAAACCATATCATACCCCA460.14119958254036466No Hit
CATAGAGTGTGTAGTGTGGTATAGCGTCAGTGTATGTGCTCGTATCTTGG460.14119958254036466No Hit
CATAGAGTGTGTAGTGTGGTATAGCGTAGCATGGTGCATGCTCCACGCTC450.1381300263981828No Hit
CATAGATGGTATTTGAGTGGGGAAACAGACACATGATGGGAGTGTCTGCT450.1381300263981828No Hit
CATAGATTCTTTCCTGGCTGTGGGTGCATAAAAACTGTCGGAGGTCTATG450.1381300263981828No Hit
CATAGAGGCTGCAGTGAACAATGATCGTCAGTGTGGTGGGTTGAGGGAGT400.12278224568727363No Hit
CATAGAGGCTGCAGTGAACAATGATCGCCATGCTGAAGTCCCATGATCTA400.12278224568727363No Hit
CATAGACCAGTACGCCTTACCCCTTGACACTGTCAGACCCAGGCTACACA400.12278224568727363No Hit
CATAGACCAGTACGCCTTACCCCTTGATGGTGGGTTGAGGGAGTCTTCTC400.12278224568727363No Hit
CATAGAAATTTTTCAGAGACAGTTGGGACAGCCATGCTGAAGTCCCATGA390.11971268954509177No Hit
CATAGAGGTGAGCGGCTGTCTCCACAGAGACAGTTGTGGAAAGTGCAATG360.11050402111854626No Hit
CATAGACCCTCTCTTACCCTTCCACGAATGAGGAAACAGGCACTCTATGT350.10743446497636443No Hit
CATAGAGGCTGCAGTGAACAATGATCGATCCGCTGCACTCCAGGGAAACT350.10743446497636443No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCGGGTCA330.10129535269200074No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)