FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005806413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005806413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66075
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCTGCG1492822.59250851305335No Hit
CATAGAGAGATTTTGGGCTGAGACGATAATGCCGCATATCTACAACTATC62519.46046159667045No Hit
CATAGAGAGATTTTGGGCTGAGACGATGGGGTTTTCTAGATATACAACAA37715.707150964812713No Hit
CATAGACACCATGCCTGGCTAATTTTTGTATTTTTAGTAGAGATAGGGTT19362.9300037835792656No Hit
CATAGACACCATGCCTGGCTAATTTTTTGCGTTTTTAGTAGAGATGGGGT17652.6712069617858494No Hit
CATAGACCTGGGCTTAGGGGCTACACATGGCTATGCAATCTATGAGACCA13982.11577752553916No Hit
CATAGAACTTCACAAGGGCTTTAGGGGCTACACATGGCTATGCAATCTAT13181.9947029890276202No Hit
CATAGAAAACAGAGTCACAGTCCCCAGTCAATTCCCAGACATAAGCCAGT12651.9144911085887248No Hit
CATAGACCTGGGCTTAGGGGCTATACATGGCTATGCAATCTATGAGACCA10551.5966704502459326No Hit
CATAGAGAGATTTTGGGCTGAGACGATGGGGTTTTCTAGATATACACTCA9781.4801362088535754No Hit
CATAGAGAGATTTTGGGCTGAGACGATGGGGTTTTCTAGATATACAATCA8471.2818766553159289No Hit
CATAGATGCCCTTTTTGTGTACTTCCATGACGACAGGTTCTACACATAGA5930.8974650018917896No Hit
CATAGACACCATGCCTGGCTAATTTTTGTATTTTTAGTAGTGGTGTGGTG5770.8732500945894817No Hit
CATAGACAGGGAGGCGTATAAGTGATGACTGATACAGGAAGGCCTTTCTA5520.8354143019296254No Hit
CATAGATCCTCTCATTGTCCAGTTTGGAGACAGCCGCTCACCTCTATGAG5320.8051456678017405No Hit
CATAGAAACCCCAAAGGCTATGTTCATTCTATGCCTTCATTCTGCCTGCA5150.7794173287930383No Hit
CATAGATGCCCTTTTTGTGTACTTCCACACTGACTGATACAGGAAGGCCT4630.7007188800605373No Hit
CATAGACACCATGCCTGGCTAATTTTAAAATTAGTACCTATAATCTCAGC4450.6734771093454408No Hit
CATAGACACCATGCCTGGCTAATTTTTTGTATTTTTAGTAGAGATGGGGT4390.6643965191070753No Hit
CATAGACACCATGCCTGGCTAATTTTTGTATTTTTAGTAGAGACAGCGTT4270.6462353386303443No Hit
CATAGATCCCATCCAATAATAATGAGCAAGCAATGGGGAAAGGATTCTAT4110.6220204313280363No Hit
CATAGACCTGGGCTTAGGGGCTATACGTGAATGTGGATCAGAACTTCTAT4080.6174801362088536No Hit
CATAGATCCCATCCAATAATAATGAGCAAGCAATGGGCAAGCAATGACAG3870.5856980703745743No Hit
CATAGACAGGGAGGCGTATAAGTGATGACACTGACTGATACAGGAAGGCC3710.5614831630722664No Hit
CATAGATGCCCTTTTTGTGTACTTCCACACTGACGACATGGTTCTACACA3590.5433219825955354No Hit
CATAGAAAACAGAGTCACAGTCCCCAGTAATGTTAGCATGTCTATGAGAC3390.5130533484676504No Hit
CATAGATGTCCATCATGTGGAAGCTGGAGAGTCACTTCTTTTGATAATTC3340.5054861899356791No Hit
CATAGATGCCCTTTTTGTGTACTTCCAACACTGACTGATACAGGAAGGCC3240.49035187287173665No Hit
CATAGAGCTGGCTTCCTAACTTCCAATGGGCAAGCAATGACAGTCTATGA3130.47370412410139995No Hit
CATAGATCATGGGACTTCAGCATGGCCAACATGGTGAACCTGTCTCTACT3090.4676503972758229No Hit
CATAGACAATTAAATTTTCTTAAGGGGTTGCACAGATCATTTAAGCTCAA3050.4615966704502459No Hit
CATAGAGCCAAGGAAAGTTGAATGGAGACAGCCGCTCACCTCTATGAGAC2980.4510026485054862No Hit
CATAGACCTTTGGGTTCATATCATCTGGAGACAGCCGCTCACCTCTATGA2900.43889519485433226No Hit
CATAGATGCCCTTTTTGTGTACTTCCACTGACGACATGGTTCTACACATA2860.4328414680287552No Hit
CATAGATTGGAACTTTGCCAGCCTTACACTGACTGATACAGGAAGGCCTT2820.4267877412031782No Hit
CATAGACACCATGCCTGGCTAATTTTTGTATTTTTAGTGGTGCACATGCC2750.41619371925841847No Hit
CATAGACAGGGAGGCGTATAAGTGATGAAATCTCTACTAAAAATACAAAA2750.41619371925841847No Hit
CATAGACACCATGCCTGGCTAATTTTTGTATTTTTTGTAGAGACAGAGTT2710.41013999243284144No Hit
CATAGAAACCCCAAAGGCTATGTTCAAAATGGTCAAACAGGAGGTCTATG2680.4055996973136587No Hit
CATAGAGGATCCCATCTGAGTTGGTCAAAGTCTGTGAGTAAAGTGCTAAT2660.40257283390087023No Hit
CATAGACACCATGCCTGGCTAATTTTTAAAAAGTAGCTGGGCATGGTGGC2610.395005675368899No Hit
CATAGATCATGGGACTTCAGCATGGCCAACATGGTGAAACCCCATCTCTA2480.37533106318577375No Hit
CATAGACCTTTGGGTTCATATCATCTGGCTACACATGGCTATGCAATCTA1940.2936057510404843No Hit
CATAGATCATGGGACTTCAGCATGGCCAACATGGTGAAACCCTGTCTCTA1890.28603859250851305No Hit
CATAGATCATGGGACTTCAGCATGGCCAACATGGTGAAACCCCGTCTCTA1850.27998486568293607No Hit
CATAGAACCTCTTTTGGGGCAAAGTTCCAATCTACACAAAGTCACGAAAG1620.24517593643586832No Hit
CATAGACACCATGCCTGGCTAATTTTTTGTATTAATCCCAGCTACTCAGG1610.2436625047294741No Hit
CATAGATTTGTAAATTGCCTAGTCTTGCATACACTGACTGATACAGGAAG1600.24214907302307984No Hit
CATAGACAATTAAATTTTCTTAAGGGGTTGTACGGTTGCACAGATCATTT1590.24063564131668558No Hit
CATAGACACCATGCCTGGCTAATTTTGTATTTTTAGTAGAGACAGGGTTT1560.23609534619750283No Hit
CATAGACAGGGAGGCGTATAAGTGATGGAGGCGTATACGTGAATGTGGAT1500.22701475595913734No Hit
CATAGACACCATGCCTGGCTAATTTTTAAATATTTTGTAGAGACCGGGTT1500.22701475595913734No Hit
CATAGAGAGATTTTGGGCTGAGACGATGGCGTATTCTAAATATACAATCA1460.22096102913356036No Hit
CATAGAAAACAGAGTCACAGTCCCCAGTAATGTTAGCATGTCTATCCCCA1430.2164207340143776No Hit
CATAGAGCAACAGGGACTCTCATTCACCTTTCCCTCACTCTCTCCATCTA1410.2133938706015891No Hit
CATAGATCCCATCCAATAATAATGAGCAGAATTACCTCTGGATGGTCTAT1410.2133938706015891No Hit
CATAGATGTCAAGAGTCTAATTCCTGCTTCCTTCTGCTGGCTATTCTATG1220.18463866818009836No Hit
CATAGACACCATGCCTGGCTAATTTTTTTGTGTTTTTGGTAGAGATGGGG1190.1800983730609156No Hit
CATAGATTTGTAAATTGCCTAGTCTTGCATATGTCTTTATCAGCAGCATT1170.17707150964812712No Hit
CATAGAACTTCACAAGGGCTTTAGGGCTACACATGGCTATGCAATCTATG1130.17101778282255015No Hit
CATAGACACCATGCCTGGCTAATTTTTGTATTTTTCGTAGAGATGGGGTT1070.16193719258418465No Hit
CATAGAAACCCCAAAGGCTATGTTCAGTGTGTGCTGGCTTGACTTTTCAT1050.15891032917139614No Hit
CATAGAAAACAGAGTCACAGTCCCCAGTGTTGATCTATCCAGGAATTTTA1030.15588346575860765No Hit
CATAGATCATGGGACTTCAGCATGGCCAAGTCTACTAAAAATACAAAAAT990.14982973893303064No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTCTGCAGATTTGGACCTGCG990.14982973893303064No Hit
CATAGATCCCATCCAATAATAATGAACAAGCAATGGGGAAAGGATTCTAT950.14377601210745364No Hit
CATAGAGAGATTTTGGGCTGAGACGATGGGGTTCTCTAAATATACACTCA950.14377601210745364No Hit
CATAGAGCAACAGGGACTCTCATTCAGTGTACAAGCAATGGGGAAAGGAT940.1422625804010594No Hit
CATAGAGAGATTTTGGGCTGAGACGATGAGGTTTTCTAAATATACAATCA910.13772228528187666No Hit
CATAGACACCATGCCTGGCTAATTTTTTAAAAATTGATTTTTGTAGAGAC880.13318199016269391No Hit
CATAGATCATGGGACTTCAGCATGGCCAACACGGTGAAACCCTGTCTCTA840.1271282633371169No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCCGCG810.12258796821793416No Hit
CATAGATCATGGGACTTCAGCATGGCGGCGTTTGCAGATTTGGACCTGCG790.11956110480514567No Hit
CATAGACCTTTGGGTTCATATCATCTGGACACTGACTGATACAGGAAGGC790.11956110480514567No Hit
CATAGACACCATGCCTGGCTAATTTTTGTATTTTTAGTAGAGACAGGGTT780.11804767309875142No Hit
CATAGACCAATAAAAGTTGATGGGATGAAGTAAGAAACATTTTAGAGCAC770.11653424139235716No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATCTGGACCTGCG770.11653424139235716No Hit
CATAGATTGGAACTTTGCCAGCCTTACCAACAAACCCCATCTCTACCAAA770.11653424139235716No Hit
CATAGATGTCCATCATGTGGAAGCTGCCTTCCTAACCATGGGCATATCTA760.11502080968596293No Hit
CATAGATGTCAAGAGTCTAATTCCTGCTCCGTAACCTGATTGGCCATTTG730.11048051456678018No Hit
CATAGAGCTGGCTTCCTAACTTCCAACCTTCCAGCAAGCCTATTAACCTT700.10594021944759742No Hit
CATAGATGTCAAGAGTCTAATTCCTGCTCTCTACACATAGATTTGTAAAT670.10139992432841467No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)