FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005806504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005806504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377465
Sequences flagged as poor quality0
Sequence length150
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA7690020.372749791371387No Hit
CATAGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG252196.681149245625423No Hit
CATAGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG191015.060336719960791No Hit
CATAGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT148963.9463261494443196No Hit
CATAGATGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG127183.3693190097095096No Hit
CATAGACCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC123843.280833984607844No Hit
CATAGACACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG110352.9234498562780655No Hit
CATAGACCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG95102.5194388883737564No Hit
CATAGACCAGCTTTGCATGAGTGTGGTTAATGAAGCTCTTCCATGTCCCA85122.2550435139681824No Hit
CATAGAGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA58901.5604095743976263No Hit
CATAGACACACCCAACTCTACTCAGTCTGCATAGCCACATCTATGTGTAG54911.4547044096803676No Hit
CATAGAGGGGAGACACTGGCTATAAGAAGACACTGGGGGCACAAAGTTCT34160.9049845681056521No Hit
CATAGATGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT32250.8543838501582928No Hit
CATAGACTTACTGCCCTCACTCCGTCTGCATAGCCACATCTATGTGTAGA31430.8326599817201595No Hit
CATAGAGGGGAGACACTGGGGGCACAAAGTTCTATGTGTAGAACCATGTC30660.8122607394063026No Hit
CATAGACTGGCGGTGATTGCTTTCCTCTCATAGACACTGGGGGCACAAAG27550.729868994476309No Hit
CATAGAGTTTGCCCTTCCTTCCCTCCACGGCAAACACTCAAAACAGCCCT23970.6350257639781172No Hit
CATAGAGTCTCAATTATTCATCTCAACATGCTACACTGATGTGGCATGGA23570.6244287549839058No Hit
CATAGAGTTTGCCCTTCCTTCCCTCCCAGAGACTTGGCCTCAGTTTCCCC23410.6201899513862212No Hit
CATAGACTGGCGGTGATTGCTTTCCTCCACGGCAAACACTCAAAACAGCC23260.616216073013392No Hit
CATAGACCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT22930.6074735405931676No Hit
CATAGAGGGGAGACACTGGCTATAAGAAGGGGAGACACTGGGGGCACAAA22560.597671307273522No Hit
CATAGAGTTTGCCCTTCCTTCCCTCCTACTTGGCCTCAGTTTCCCCTCCT22450.5947571298001139No Hit
CATAGACCAGCTTTGCATGAGTGTGGCATGGAGAAGGGTCTATGTGTAGA21030.5571377478706635No Hit
CATAGATTAGGGGTGACGGACTAGCCCTTGGCCTCAGTTTCCCCTCCTTC20030.530645225385135No Hit
CATAGAGATGATGGGATCTTATATTTGGAAAAACTTACGGTAGCAAAGCT19200.5086564317221465No Hit
CATAGACCAGCTTTGCATGAGTGTGGAACACCAGTGTAGCAGACCATCTT19060.5049474785741724No Hit
CATAGAGTTATTCAGAGAGAGGGGAAATGAGAGAAAACTACCCCAGGCTC18450.48878703985800004No Hit
CATAGACTTACTGCCCTCACTCAGTCTGCATAGCCACATCTATGTGTAGA18060.47845495608864397No Hit
CATAGACCAAACACAGAAGACATGGCATGTACCAGTGTAGCAGACCATCT17580.4657385452955903No Hit
CATAGAGTTTGCCCTTCCTTCCCTCCAAGCAGATCTGTGTTCTATGTGTA15030.3981826129574927No Hit
CATAGAGGGGAGACACTGGCTATAAGAACTTGGTCTCATAGAGGGGAGAC14930.3955333607089399No Hit
CATAGAGTCTCAATTATTCATCTCAACATGCTATGATGTGGCATGGAGAA14480.3836117255904521No Hit
CATAGAGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT14400.38149232379160986No Hit
CATAGACCAAACACAGAAGACATGGCAAACACTCAAAACAGCCCTCTATG14310.3791079967679123No Hit
CATAGAGGGGAGACACTGGCTATAAGAAGACTTGGTCTCATAGAGGGGAG14300.37884307154305696No Hit
CATAGATGACCTGTTAATCTGCCCCCGTAACCTAGTGATTACCTGGGTGA14270.37804829586849115No Hit
CATAGACTTTTCCTCCAATATAGCTGCACTGACTTGGCCTCAGTTTCCCC14260.3777833706436358No Hit
CATAGATTAGGGGTGACGGACTAGCCTAGCCGACTTGGCCTCAGTTTCCC13880.367716212099135No Hit
CATAGATGTTTGAAACTCCTCTACCTAAGAGACAACACAGGATCCAGGTG13390.3547348760812261No Hit
CATAGATGTTTGAAACTCCTCTACCTAAGACAACACAGGATCCAGGTGTC12740.33751473646563257No Hit
CATAGAGATGATGGGATCTTATATTTGGAAAAACTGGCAAAGCTGCTATG12730.3372498112407773No Hit
CATAGACTGGCGGTGATTGCTTTCCTAGACTTGGCCTCAGTTTCCCCTCC12440.32956697971997406No Hit
CATAGAGGGGAGACACTGGCTATAAGAAGGGAGACACTGGGGGCACAAAG11900.3152610175777887No Hit
CATAGAGGCTGGGTGACTTGGTGAATGTTGGAGAGGCACATCTATGTGTA11490.304399083358722No Hit
CATAGAGATGATGGGATCTTATATTTGGAAAAACTGGTAGCAAAGCTGCT10330.27366775727550896No Hit
CATAGAGATGATGGGATCTTATATTTGGAAAAACTATTAGCGAGACTCTA10280.27234313115123254No Hit
CATAGAGGTGAGCGGCTGTCTCCATCAATTTAAGGTAGGCACTTCCTCTA9410.24929463658882278No Hit
CATAGACCTCCTTTCCTCCACGGCAAAGCTGCTATGGGATCTTCTATGTG8510.2254513663518472No Hit
CATAGAGATGATGGGATCTTATATTTGGAAAAACTATTAGCAAAGCTGCT8480.22465659067728133No Hit
CATAGAGTCTCAATTATTCATCTCAACATGCTGATGTGGCATGGAGAAGG8300.21988793662988623No Hit
CATAGAGGGGAGACACTGGCTATAAGAAGAGGGGAGACACTGGGGGCACA7750.20531704926284558No Hit
CATAGAGTGGGGGACTAGCATTGATTGAGTCTATGTGTAGAACCATGTCG7440.19710436729233174No Hit
CATAGATGGTGGGTTCTGGAGAGAGGGCGAAAGTGATCAGAAGCTCTATG7430.19683944206747647No Hit
CATAGAGGGGAGACACTGGCTATAAGAATAGAGGGGAGACACTGGGGGCA7410.19630959161776587No Hit
CATAGACCAGCTTTGCATGATGTGGCATGGAGAAGGGTCTATGTGTAGAA6630.17564542407905367No Hit
CATAGACACCTTGTATATGCCAGGACTAGATCGGAAAGCTGTCTGTGTAG6530.17299617183050084No Hit
CATAGAGGGGAGACACTGGCTATAAGAAGTCTCATAGAGGGGAGACACTG6350.1682275177831057No Hit
CATAGAGTTATTCAGAGAGAGGGGAAATGAGACCAGATTTATCCAGCATG5980.1584252844634602No Hit
CATAGACACACCCAACTCTGCTCAGTGTACTCAGTCTGCATAGCCACATC5850.15498125654034148No Hit
CATAGACTTCTGTTTGGTCACATGCTCAGTGTGGAAAGCTGTCTGTGTAG5700.15100737816751222No Hit
CATAGATGGTGGGTTCTGGAGAGAGACACTGGGGGCACAAAGTTCTATGT5670.15021260249294635No Hit
CATAGACTTACTGCCCTCACTCCGTCTACGGTAGCAAACACTCAAAACAG5430.14385439709641953No Hit
CATAGATGATGTTTTAAAATGAGCACCTAACATCTATGTGTAGAACCATG5100.13511186467619513No Hit
CATAGATCCACTGTGCTATCTCTGACAGACTTGGCCTCAGTTTCCCCTCC5010.1327275376524976No Hit
CAGTTTCCCCTCCTTCTATGTGTAGAACCATGTCGTCAGTGTAGATCTCG5000.1324626124276423No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCGGGTCA4930.1306081358536553No Hit
CATAGATACGATCATAAGGGTGGGGCTGGGCTCAGTGGCTTATGCCTGTA4710.12477978090683906No Hit
CATAGATGACCTGTTAATCTGCCCCCACTCAGTCTGCATAGCCACATCTA4550.1205409773091545No Hit
CATAGAGTCTCAATTATTCATCTCAACATGCCATGCTGAAGTCCCATGAT4470.11842157551031221No Hit
CATAGAGTTTGCCCTTCCTTCCCTCCTTCTATGTGTGGCCTCAGTTTCCC4420.11709694938603579No Hit
CATAGATTAGGGGTGACGGACTAGCCGACTCAGTCTGCATAGCCACATCT4360.11550739803690409No Hit
CATAGACCTGACCTCATGATCTGCCTGCCTCGGCCTCCCAAAGTGCTGGG4330.11471262236233823No Hit
CATAGACCTGACCTCATGATCTGCCTACCTCAGCCTCCCAGATTGCTGGG4090.1083544169658114No Hit
CATAGATGATGTGAAAAGGACCCATGGCGTCAGTGTAGGTGGGAGGATTG4070.10782456651610084No Hit
CATAGACCTGACCTCATGATCTGCCTTCAGAACGCGGCAAATGGTCTATG4070.10782456651610084No Hit
CATAGACTTTTCCTCCAATATAGCTGCACTTACGGTAGCAGAGACTCAGT4000.10597008994211385No Hit
CATAGACTTTTCCTCCAATATAGCTGCACTTAATCTATGTGTAGAACCAT4000.10597008994211385No Hit
CATAGAGGTGAGCGGCTGTCTCCAGAAGTCCGCGCAGAGCCTTCAGGTCA3970.10517531426754798No Hit
CATAGATTAGGGGTGACGGACTAGCCACACTCAGTCTGCATAGCCACATC3790.10040666022015285No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)