FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005806525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005806525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117662
Sequences flagged as poor quality0
Sequence length150
%GC37

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGAGCTTGGGTGCGAACATCAAGGAAGTTTCTGTGAGATCTATGAGA1362211.577229691829137No Hit
CATAGAGCTTTTTCTCCAGCCTCCAAGTAATAACCAGGCCCCTCTATGAG93217.92184392582142No Hit
CATAGAGAAGACTCCCTCAACCCACCCACAGCCAGGAAAGAATCTATGAG79816.782988560452822No Hit
CATAGAAATGATGCTGAAGGGCCTGCCCTCTCTGCTGTTCCTTCTATGAG69295.888902109432102No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCTGCG47474.034437626421444No Hit
CATAGACGGATTATTCTCTCCATGGTCTACCAGTTATTAGTTGAATGAGC47074.000441943873128No Hit
CATAGACTCATTGCACTTTCCACAACTGTGTGCTTGAACCAGATTCTATG37763.209192432561065No Hit
CATAGATGTTTTATCAAAGGCATGCTCATCAGGAACCATGGGAGGTCTAT35923.0528122928388095No Hit
CATAGATGTTTTATCAAAGGCATGCTCATCAAGGAAGTTTCTGTGAGATC31372.666111403851711No Hit
CATAGAGGAGGTGAAAGAAATCTACAATGGGTTCTGGGCCCCAGTGTGAA30682.6074688514558653No Hit
CATAGACTCATTGCACTTTCCACAACTGTCATTGTCGCTGTCCCATCTAT28972.462137308561813No Hit
CATAGATCATGGGACTTCAGCATGGCTCAGGTATGTCTTTATTAGCTCTA26732.2717614862912408No Hit
CATAGAAATGATGCTGAAGGGCCTGCTGAAGTGATGGGCTAGATCTATGA20841.7711750607672827No Hit
CATAGAAGTCACGAGTTTTGTTGTTGTTCTTCCTTTTCTGCCTTCATCTA20711.7601264639390797No Hit
CATAGAGTGCCTGTTTCCTCATTCGTCTGTTTCCCCACTCAAATACCATC14991.2739882034981558No Hit
CATAGACACAACAGAGAGAACGGGCTACACTGACGCTATACCACACTACA14561.2374428447587156No Hit
CATAGACTCATTGCACTTTCCACAACTGTCCCAACTGTCTCTGAAAAATT13071.1108089272662371No Hit
CATAGACTCATTGCACTTTCCACAACTGTACACTGACGCTATACCACACT12681.0776631367816287No Hit
CATAGAGGAGGTGAAAGAAATCTACAATGGGCCCCAGTGTGAATACTTCT12291.0445173462970203No Hit
CATAGACCAACATTTCTTCTCCCAGGGTACACTGACGCTATACCACACTA9870.8388434668797063No Hit
CATAGACTCATTGCACTTTCCACAACTGTCTGCCTCTAAGTAAACTGAAT9350.794649079566895No Hit
CATAGAGTCTGAATGGTGGGAGAGTGAGGAGCTGAGTCATGGGGTCTATG9120.7751015621016131No Hit
CATAGAAGCCATGAACACAGGATTGAGGAGCTGAGTCATGGGGTCTATGA8690.7385562033621731No Hit
CATAGACGTGACTAAAAGCATGTTGGTGGAAGGGTAAGAGAGGGTCTATG7480.6357192636535159No Hit
CATAGATCAAGGAAAACACCAGCCCACGCTGTCCTTCTCTGAATCTATGA7140.6068229334874471No Hit
CATAGATGTTTTATCAAAGGCATGCTCATTTATTTTAAAGGACTGGAAGC7120.6051231493600312No Hit
CATAGATCACGTAGAAACTGGAGAGGCTAATCTGAAATCTTCGTGTTCTA7080.6017235811051996No Hit
CATAGATGGCCAAGATACGAGCACATACACTGACGCTATACCACACTACA6180.5252332953714878No Hit
CATAGACCAGCAGACACTCCCATCATCAGGAACCATGGGAGGTCTATGAG6090.5175842667981166No Hit
CATAGAAATGATGCTGAAGGGCCTGTTTCCCCACTCAAATACCATCTATG5540.47084020329418164No Hit
CATAGAGAAGACTCCCTCAACCCACGCTGTCCTTCTCTGAATCTATGAGA5270.4478931175740681No Hit
CATAGATCAAGGAAAACACCAGCCCAACACTGACGCTATACCACACTACA5230.4444935493192364No Hit
CATAGACCAACATTTCTTCTCCCAGGGTATGATGCTGAAGTGATGGGCTA5090.43259506042732576No Hit
CATAGACACAACAGAGAGAACGGGCTACACTGACGATCATTGTTCACTGC5040.4283456001087862No Hit
CATAGAAAATTTGTGATGGTAAATCATGGGACATGCCAGGAATCAATGTT4740.40284883819754885No Hit
CATAGATCAAGGAAAACACCAGCCCATGCAAACTGTTCTAGGCAGAAGAA4560.38755078105080654No Hit
CATAGACTCATTGCACTTTCCACAACTGTACACTGACGATCATTGTTCAC4500.3824514286685591No Hit
CATAGACCAGCAGACACTCCCATCATTTATTTTAAAGGACTGGAAGCCTC4180.355254882629906No Hit
CATAGAGTCCAACTATTCCAGCACCATGCTGAAGTGATGGGCTAGATCTA3810.3238088762727134No Hit
CATAGAAGGACAAAGACATTTAGGACCCAGCAAGGGGTAAGGCGTACTGG3680.3127602794445105No Hit
CATAGAGTCTGAATGGTGGGAGAGTGAATGCTGAAGTGATGGGCTAGATC3540.3008617905525998No Hit
CATAGACTCATTGCACTTTCCACAACTGTAATGATGCTGAAGTGATGGGC3500.2974622222977682No Hit
CATAGAGAAGACTCCCTCAACCCACCACACTGACGCTATACCACACTACA3380.2872635175332733No Hit
CATAGAAATGATGCTGAAGGGCCTGCTACACATAGAAATGATGCTGAAGT3360.28556373340585745No Hit
CATAGACCAGCAGACACTCCCATCATGCTGAAGTGATGGGCTAGATCTAT3290.2796144889599021No Hit
CATAGAGTGCCTGTTTCCTCATTCGTACACTGCCCTCTCTGCTGTTCCTT3230.27451513657765464No Hit
CATAGAGTGCCTGTTTCCTCATTCGTACACTGACGATCATTGTTCACTGC3230.27451513657765464No Hit
CATAGAAGGACAAAGACATTTAGGACCCAGGGGTGGAAGGGTAAGAGAGG3220.27366524451394675No Hit
CATAGAGTCCAACTATTCCAGCACCACACTGACGATCATTGTTCACTGCA3210.2728153524502388No Hit
CATAGACCCAAGACCCCAGCCTTTACACACTGACGCTATACCACACTACA3190.27111556832282296No Hit
CATAGACTCATTGCACTTTCCACAACTGTACACTGTGTGCTTGAACCAGA3140.26686610800428345No Hit
CATAGATGGCCAAGATACGAGCACATACACTGACGATCATTGTTCACTGC3130.2660162159405755No Hit
CATAGAGTCTGAATGGTGGGAGAGTGAAAGAAATCTACAAAGGATTCTGG3080.261766755622036No Hit
CATAGAAAGGTGCAATCAAGCCAGTTACACTGACGCTATACCACACTACA3050.25921707943091227No Hit
CATAGAGTCCAACTATTCCAGCACCACACCCACAGCCAGGAAAGAATCTA3020.25666740323978854No Hit
CATAGACATTTAATCCTCACAATGACCCATACACTGACGATCATTGTTCA2810.23881966990192244No Hit
CATAGACGGATTATTCTCTCCATGGTCTACAAAGGATTCTGGGCCATCTA2790.23711988577450666No Hit
CATAGACCTCCGACAGTTTTTATGCACCCACAGCCAGGAAAGAATCTATG2650.2252213968825959No Hit
CATAGAGCTTTTTCTCCAGCCTCCAAGGGGTAAGGCGTACTGGTCTATGA2490.21162312386326937No Hit
CATAGAGCTTGGGTGCGAACATCAACACACTGGCTCAGGTATGTCTTTAT2490.21162312386326937No Hit
CATAGAAGGACAAAGACATTTAGGACCCACACTGACGCTATACCACACTA2460.20907344767214564No Hit
CATAGAAGGACAAAGACATTTAGGACCCACATTGCTTCTTGTGGTAACTG2450.20822355560843772No Hit
CATAGATGTTTTATCAAAGGCATGCTCATGGAGGTGAAAGAAATCTACAA2430.2065237714810219No Hit
CATAGACCAGCAGACACTCCCATCATCAAGGAAGTTTCTGTGAGATCTAT2390.20312420322619026No Hit
CATAGACGGATTATTCTCTCCATGGTCTGAGGAGAATTCAAGCTGCTCTA2290.1946252825891112No Hit
CATAGAAATGATGCTGAAGGGCCTGCAGAAGTAGGAAGTATTACCCCTCT2250.19122571433427954No Hit
CATAGAAGCTGAGAAGACTTCCTAATGCAGAAGTAGGAAGTATTACCCCT2040.17337798099641347No Hit
CATAGATGTTTTATCAAAGGCATGCTCATACACTGACGATCATTGTTCAC2020.17167819686899766No Hit
CATAGACCAACATTTCTTCTCCCAGGGTATAAAGCTGGACCCTCTCTGGG2010.17082830480528974No Hit
CATAGAAAAAACAGTTGGCCAGGCATTGCTTCTTGTGGTAACTGGTCTAT1990.16912852067787393No Hit
CATAGACCGCTTCCCAATTTTTGTAACAACACTGACGCTATACCACACTA1830.15553024765854737No Hit
CATAGAACAGATGTTCTCCTTGTTTTCTATAACCGTATCTGAGGAGAATT1830.15553024765854737No Hit
CATAGAGTGCCTGTTTCCTCATTCGTGTCTGTTTCCCCACTCAAATACCA1740.14788121908517618No Hit
CATAGAGCTGTACTGCATCTTTTCTCAAACTGCTGGGGTTGCTCTATGAG1620.13768251432068127No Hit
CATAGACACAACAGAGAGAACGGGCTACACTGACGGACATGCCAGGAATC1600.13598273019326546No Hit
CATAGAGTCCAACTATTCCAGCACCACACTGACGACATGGTTCTACACAT1600.13598273019326546No Hit
CATAGAGCTGTACTGCATCTTTTCTCAGACATGGTTCTACACATAGAGTG1400.11898488891910726No Hit
CATAGATGTTTTATCAAAGGCATGCTCATACACTGACGACATGGCTCAGG1360.11558532066427564No Hit
CATAGAAATGATGCTGAAGTGATGGGCTAGATCTATGAGACCAAGTCTCT1320.11218575240944399No Hit
CATAGACGGATTATTCTCTCCATGGTCTGTTTCCCCACTCAAATACCATC1280.10878618415461237No Hit
CATAGATGGCCAAGATACGAGCACATGAGCAGCATAATTTCCCATGTCAT1280.10878618415461237No Hit
CATAGACCGCTTCCCAATTTTTGTAACATAGAGATCCCACCATTTGCCAT1260.10708640002719655No Hit
CATAGACTCATTGCACTTTCCACAACTGTCTCATTGCTTCTTGTGGTAAC1230.10453672383607282No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)