FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005806532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005806532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156174
Sequences flagged as poor quality0
Sequence length150
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGAGCAGTATCCCAGTTAGCTCCTGACAATACCGCGTCTATGTGTAG102186.542702370432979No Hit
CATAGAAGAGAACCAAAAATGGACCCAGAAGAGAAGTACCAGGTCTATGT74424.76519779220613No Hit
CATAGATTCCCCTCATACCACGCCTCCCTCTACCAGCCTCAGTTCTATGT73104.680676681137705No Hit
CATAGAGGTGAGCGCCAGTCTCCCAGTAATCTATGTGTAGAACCATGTCG59123.7855212775494No Hit
CATAGAAAAGTGTATTATGGGCCCAGGAGTTGACAAACGACAGCTTCTAT46002.9454326584450676No Hit
CATAGATGAGGGATGTAGGCTGTACATGTACACGGCAGGACTGTCTATGT42602.727726766299128No Hit
CATAGAAAAGGGAGGGATTACGGGTGACACCAAAAGCACAAGTTCTATGT42402.7149205373493666No Hit
CATAGACGTGTGGTTGGGATGTCAGCTCCTCTTCTCTTCCCTCTATGTGT39612.5362736435001985No Hit
CATAGATGGGTCCCATTCTGTTTGCAGGATGAATGGGGACATCTATGTGT36652.3467414550437335No Hit
CATAGAACATCTCCCTGTTCCTCCCACCTAAACTCCTGAGATCCTCTATG30751.968957701025779No Hit
CATAGAGGTGAGCGGCTGTCTCCAGTAATCTATGTGTAGAACCATGTCGT25261.617426716354835No Hit
CATAGAACTCTTACCCTTTTGCCAGGGCTGCATGGTGACTTTTCTATGTG24941.5969367500352172No Hit
CATAGAAAAGTGTATTATGGGCCCAGGGCTGCATGGTGACTTTTCTATGT23331.4938466069896397No Hit
CATAGAAGGATAAAGGCGGAAGAGAGCCGTTTCCCCCATACTGTCTATGT18571.189058357985324No Hit
CATAGAAGGATAAAGGCGGAAGAGAGCATAATCTATAGGAGTTGACAAAC16761.0731619859899855No Hit
CATAGAGCAGTATCCCAGTCAGCTCCTGACAATACCGCGTCTATGTGTAG15400.9860796291316096No Hit
CATAGAACCAACCAATAATAATGTGCTTGTACACTGCTCACGATGGTATT14760.9450996964923739No Hit
CATAGAACTGTCCCCTACTTTTCACCAAGGTCAAGGGCAGGGATTCTATG14300.9156453699079232No Hit
CATAGATGAAAACATTCCGAAGCTAGATCATTGTCCTCTTCACCTCCCAG13560.868262322793807No Hit
CATAGAGGTGAGCGGCTGTCTCCCAGTAATCTATGTGTAGAACCATGTCG13440.8605785854239502No Hit
CATAGACTGGTGTAATGTGTCATATACTTGGTACGGTAGGTGGCTTCTGG13340.8541754709490695No Hit
CATAGACGTGTGGTTGGGATGTCAGCGTGTATGTGAGTGCCAAATCTATG12820.8208792756796905No Hit
CATAGAACTGACCATACACTTGCCCTTCTGTCTAGGATGAGATAATTGCC12650.8099939810723936No Hit
CATAGAGGCCATTCTTCTCATGCTGAAGGATAGTTGGGTGCTCTATGTGT12610.8074327352824413No Hit
CATAGAATAGCTGTGTTGAAGAACCTCAAGAAAGAAAACCCACAAAAGAC11240.7197100669765774No Hit
CATAGATTCTGTTTCTCTTCCCCCAAGGTCAAGGGCAGGGATTCTATGTG10820.692816986182079No Hit
CATAGAACTGACCATACACTTGCCCTTCGTCTAGGATGAGATAATTGCCC10430.6678448397300447No Hit
CATAGAGCTTCAGATTCCATAAGTCATCCTACGGTAGGTGGCTTCTGGTG10270.6575998565702358No Hit
CATAGATCCATGGTGTGCAAGGACAATCACAGGGCTGCATGGTGACTTTT10140.649275807752891No Hit
CATAGAAAAGTGTATTATGGGCCCAGGAGGAGTTGACAAACGACAGCTTC10050.6435130047254984No Hit
CATAGAGGCCATTCTTCTCATGCTGGCATTCTTTCTCCAGACCTTCATCT9160.5865252858990613No Hit
CATAGATTCTGTTTCTCTTCCCCCAAAGCAGCAAACAATTTCAGAAAGTC8780.5621934508945151No Hit
CATAGACACTTGACCATGCCCTTGTTCGATGACACTTGAAAGCTCCCTGG8560.5481065990497778No Hit
CATAGATCCATGGTGTGCAAGGACAAGGGCAGGGCTGCATGGTGACTTTT8370.5359406815475047No Hit
CATAGACAGGGGCCAGAACTCCTAAGTACGGTTGCAGGATGAATGGGGAC8320.5327391243100644No Hit
CATAGATGGGTCCCATTCTGTTTGCATAGATGGGTCCCAAAGTAGGCAAT8190.5244150754927197No Hit
CATAGATGGGTCCCAAAGTAGGCAATTCTATGTGTAGAACCATGTCGTCA8160.5224941411502555No Hit
CATAGATCCATGGTGTGCAAGGACAAGGGCAGGGATTCTATGTGTAGAAC8070.516731338122863No Hit
CATAGATGGGTCCCATTCTGTTTGCACAGGACATTGGGGAATTCTATGTG7970.5103282236479824No Hit
CATAGAATAGCTGTGTTGAAGAACCTCATGCCAAGTAAGTCTACAAAGAA7810.5000832404881734No Hit
CATAGAAGGATAAAGGCGGAAGAGAGCATAATCTACAGAGTGGGAGTCAT7540.4827948314059959No Hit
CATAGACACTTGACCATGCCCTTGTTCTACGGTTGCAGGATGAATGGGGA7520.4815142085110197No Hit
CATAGAAGGATAAAGGCGGAAGAGAGCATTCTTTCTCCAGACCTTCATCT7510.4808738970635317No Hit
CATAGAACTGACCATACACTTGCCCTTACGGTTGCAGGATGAATGGGGAC7490.4795932741685556No Hit
CATAGAAAAGGGAGGGATTACGGGTGTACGGTAGCAGAGTGGGAGTCATT7440.4763917169311153No Hit
CATAGAAGGGGTGATGCTCTGATGGATCTCAGAGTTAGCTTGCCTCTATG7110.45526143916400935No Hit
CATAGAACTAGTTCCAGCACCACCAGGGCTGCATGGTGACTTTTCTATGT6760.43285053850192734No Hit
CATAGACTGGTGTAATGTGTCATATACTTGGGTAGAATCTTGAACTCAGG6750.4322102270544393No Hit
CATAGACTGGTGTAATGTGTCATATACTTGGTAGGTGGCTTCTGGTGCAT6700.429008669816999No Hit
CATAGATGAGGGATGTAGGCTGTACACGGCAGGGCTGCATGGTGACTTTT6660.4264474240270467No Hit
CATAGACTCAGAACTGGCTCTAATTCCTGGATCTCAGAGTTAGCTTGCCT6500.41620244086723784No Hit
CATAGAACCAACCAATAATAATGTGCTTGTTGATTTAGGAAGCAGGGTTC6480.4149218179722617No Hit
CATAGAGGGATGGTATCAGAGAAGGCTACATAGATCTGCTCACGATGGTA6370.40787839204989307No Hit
CATAGACAGGGGCCAGAACTCCTAAGGACAGGGGTGTGGTGTTCTGTCTA5960.3816256227028827No Hit
CATAGACAATTTCAGTGCGGCCGATGGTTTGTAAAAGCAGCAAACAATTT5900.37778375401795433No Hit
CATAGAACTAGTTCCAGCACCACCAGTTAGGCTACTTGGGGGTCTCTATG5860.37522250822800207No Hit
CATAGAACTGACCATACACTTGCCCTTTTGCCAAGTAAGTCTACAAAGAA5860.37522250822800207No Hit
CATAGATGGGTCCCATTCTGTTTGCAGAGACTTGGTCTCATAGATGGGTC5550.3553728533558723No Hit
CATAGATTCTGTTTCTCTTCCCCCAAACCTGTGTGTGTAAGTGGGTCTAT5510.35281160756592006No Hit
CATAGACCAGGACAATCACAGGGCACGGTCAAGTGTCAAGAAAGAAAACC5500.352171296118432No Hit
CATAGACGTGTGGTTGGGATGTCAGCTCCTGACAATACCGCGTCTATGTG5470.3502503617759678No Hit
CATAGATGGGTCCCATTCTGTTTGCATTCTTTCTCCAGACCTTCATCTAT5340.3419263129586231No Hit
CATAGAGCTGAGAAGGTAGGTAAAGGGGTACACATGTACACGGCAGGACT5240.3355231984837425No Hit
CATAGAAGGATAAAGGCGGAAGAGAGCTACGGCAGCTCCTCTTCTCTTCC5180.33168132979881415No Hit
CATAGAACCAACCAATAATAATGTGCTTGTCCTCTTCACCTCCCAGATCT5150.32976039545634994No Hit
CATAGAAGGATAAAGGCGGAAGAGAGCGCCAGTCTCCCAGTAATCTATGT4940.31631385505910076No Hit
CATAGAACTCTTACCCTTTTGCCAAGTAAGTCTACAAAGAACCTCTATGT4840.3099107405842202No Hit
CATAGATCCATGGTGTGCAAGGACAAGGACAGGGGTGTGGTGTTCTGTCT4830.30927042913673214No Hit
CATAGAGGCCCAGGAGAGTTGCTTAATACGGTAGGTGGCTTCTGGTGCAT4680.2996657574244112No Hit
CATAGAACATCTCCCTGTTCCTCCCAAGGTCAAGGGCAGGGATTCTATGT4530.2900610857120903No Hit
CATAGACACTTGACCATGCCCTTGTTCTTGGTCTCATAGACACTTGAAAG4360.2791757911047934No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA4360.2791757911047934No Hit
CATAGAGGGATGGTATCAGAGAAGGCTGTCTCTCCCTCTCTCACACATCT4330.2772548567623292No Hit
CATAGAAGGGGTGATGCTCTGATGGATACGGTTGCAGGATGAATGGGGAC4270.27341298807740083No Hit
CATAGATGGGTCCCATTCTGTTTGCACTTGGTCTCATAGATGGGTCCCAA4170.26700987360252026No Hit
CATAGAACTAGTTCCAGCACCACCAGCTGCCAGGCTAGGCCTCCAAGGCT4150.26572925070754416No Hit
CATAGAACTGACCATACACTTGCCCTTTTGCCATGCTGAAGTCCCATGAT4100.26252769347010385No Hit
CATAGAACATCTCCCTGTTCCTCCCAGATCAAGAAAGAAAACCCACAAAA4090.2618873820226158No Hit
CATAGAGGTGTTAAGTGTGCCCATTGTTACGGTTGCAGGATGAATGGGGA4040.25868582478517554No Hit
CATAGATGGGTCCCATTCTGTTTGCATGGGTCCCAAAGTAGGCAATTCTA4030.25804551333768744No Hit
CATAGAACCAACCAATAATAATGTGCTTGTGACTGGCTAATGTTTGCTAT4010.25676489044271134No Hit
CATAGATGGGTCCCATTCTGTTTGCAGCAGAGACTTGGTCTCATAGATGG3940.2522827103102949No Hit
CATAGAGTTCCAGCAAAATGAAGGGGTGATGCTCTGAGGAAGTGACATGC3850.2465199072829024No Hit
CATAGACACTTGACCATGCCCTTGTTCCAGACACTTGAAAGCTCCCTGGA3740.2394764813605338No Hit
CATAGATCCATGGTGTGCAAGGACAATAGATCCATGGTGTGCACAGGACA3560.2279508753057487No Hit
CATAGAAGGATAAAGGCGGAAGAGAGCAGCTCCTCTTCTCTTCCCTCTAT3440.22026713793589203No Hit
CATAGATCCATGGTGTGCAAGGTCAAGGGCAGGGATTCTATGTGTAGAAC3220.20618028609115474No Hit
CATAGACAATTTCAGTGCGGCCGATGTACGGTAGCAGACACTTGAAAGCT3210.20553997464366666No Hit
CATAGATCCATGGTGTGCACAGGACATTGGGGAATTCTATGTGTAGAACC3140.20105779451125025No Hit
CATAGACAATTTCAGTGCGGCCGATGACACTGAACGAAATTTAGGTGAGC2870.1837693854290727No Hit
CATAGATCCATGGTGTGCAAGGACATTGGGGAATTCTATGTGTAGAACCA2710.17352440226926377No Hit
CATAGATTCTGTTTCTCTTCCCCCAAATACGGTAGGTGGCTTCTGGTGCA2710.17352440226926377No Hit
CATAGATCCATGGTGTGCAAGGACAGGGGTGTGGTGTTCTGTCTATGTGT2670.17096315647931154No Hit
CATAGACTGGCTATTGTTTGGTTTGTACATGTACACGGCAGGACTGTCTA2600.16648097634689513No Hit
CATAGAACTTTCCCACCGCCCTTTTCTATGTGTAGAACCATGTCGTCAGT2550.16327941910945484No Hit
CATAGAGGCCATTCTTCTCATGCTGGGTCCCAAAGTAGGCAATTCTATGT2540.1626391076619668No Hit
CATAGAGAAGGAAAACTGGGCTGTGGCAGGCAGAGTGGGAGTCATTCATG2520.16135848476699066No Hit
CATAGAACTAGTTCCAGCACCACCAGAGTGGGAGTCATTCATGATCTATG2510.1607181733195026No Hit
CATAGACCAGGACAATCACAGGGCAGGGCTGCATGGTGACTTTTCTATGT2490.1594375504245265No Hit
CATAGATGGGTCCCATTCTGTTTGCAGATGGGTCCCAAAGTAGGCAATTC2480.15879723897703843No Hit
CATAGAACCAACCAATAATAATGTGCTTGTGACACCAAAAGCACAAGTTC2480.15879723897703843No Hit
CATAGACACTTGACCATGCCCTTGTTCTGGTCTCATAGACACTTGAAAGC2240.14342976423732504No Hit
CATAGACACTTGACCATGCCCTTGTTCTAGACACTTGAAAGCTCCCTGGA2140.13702664976244444No Hit
CATAGAAGAGAGCATAATCTATAGAGTTGGAACACTGAACGAAATTTAGG2130.1363863383149564No Hit
CATAGAGCAGACAATGGATGATCAAGTGACACCAAAAGCACAAGTTCTAT2130.1363863383149564No Hit
CATAGAAGGATAAAGGCGGAAGAGAGCCTCATAGATGTGGTTGCATGGGG2110.1351057154199803No Hit
CATAGATGGGTCCCATTCTGTTTGCAATCTTTGTTGATTTAGGAAGCAGG2100.1344654039724922No Hit
CATAGAACATCTCCCTGTTCCTCCCAGTTAGGCTACTTGGGGGTCTCTAT2100.1344654039724922No Hit
CATAGACGTGTGGTTGGGATGTCAGCCAAGGTCAAGGGCAGGGATTCTAT2060.13190415818253998No Hit
CATAGATCCATGGTGTGCAAGGACAATGGTGTGCACAGGACATTGGGGAA2030.12998322384007582No Hit
CATAGAACATCTCCCTGTTCCTCCCACACTGAACGAAATTTAGGTGAGCA2010.1287026009450997No Hit
CATAGATCCATGGTGTGCAAGGACAAGTGCACAGTTTGGAATGACTCTAT1950.12486073226017134No Hit
CATAGAAAAGTGTATTATGGGCCCAGGCAGCTCCTCTTCTCTTCCCTCTA1860.11909792923277883No Hit
CATAGACACTTGACCATGCCCTTGTTGACACTTGAAAGCTCCCTGGATCT1760.11269481475789825No Hit
CATAGAGAAGCCAGGGAACAAAGTGGGCAATCTATGTGTAGAACCATGTC1710.10949325752045795No Hit
CATAGAACTCTTACCCTTTTGCCAGGTAAGTCTACAAAGAACCTCTATGT1640.10501107738804154No Hit
CATAGATGGGTCCCATTCTGTTTGCAGTCTCATAGATGGGTCCCAAAGTA1610.10309014304557737No Hit
CATAGACTTGTTGCCGAGACTGGAGTACAATGGCGCTATCTTCTGCTGCA1610.10309014304557737No Hit
CATAGAGAAGCCAGGGAACAAAGTGGGTCCCAAAGTAGGCAATTCTATGT1580.10116920870311319No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)