FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005806534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005806534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74741
Sequences flagged as poor quality0
Sequence length150
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA2656235.53872707081789No Hit
CATAGATCCAGCTTCATCCATGTCCCGTGGTGTGCAAATTCTCTATGTGT31294.186457232308906No Hit
CATAGAAAGGGACAAGGGCTCACTCTAGCCTGGGAGACATCTATGTGTAG29984.011185293212561No Hit
CATAGAACCAACCCTGATTCTGCTGCTCACTCTTTGGGTTCTATGTGTAG14771.9761576644679628No Hit
CATAGAAAGGGACAAGGGCTCACTCTTTGGGTTCTATGTGTAGAACCATG14761.9748197107344028No Hit
CATAGAACAATCAGGCACCCACCATGCTGAAGTCCCATGATCTATGTGTA13371.7888441417695775No Hit
CATAGATGTTGTTGTTCTGTTGCCCAATATGAACAGAAAGAAGCTCTATG7921.0596593569794357No Hit
CATAGATCAAGGTGTGAGCATGGTCCCGTGGTGTGCAAATTCTCTATGTG7761.0382520972424774No Hit
CATAGATCTCAAACAGCCTCTCCCAGGAAAGTTGGAACCTATCAGTGTCT7681.027548467373998No Hit
CATAGAGGCGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA7661.0248725599068784No Hit
CATAGATGAAAGAGCATTGGTTGTAACACCGAAAAACACCCTCTATGTGT7521.0061412076370397No Hit
CATAGAGCCATCATCATTTCTCACCCAGGAAAGTTGGAACCTATCAGTGT7300.9767062254987222No Hit
CATAGACTCACTCTGTCACCCAAGCTGGAGTGCAGTGGTGTGATCATATC6760.9044567238864881No Hit
CATAGATCTCAAACAGCCTCTCCCAGGGTAATTCTGGCCTTGTTCTATGT5980.8000963326688162No Hit
CATAGAGGTGTTTCTTATAGTCAACTGAAGTGTCTCATAGAGGCAAGGGT5270.7051016175860638No Hit
CATAGATGTTGTTGTTCTGTTCCCCAATATGAACAGAAAGAAGCTCTATG5260.703763663852504No Hit
CATAGATAGCAGCAATCTCAGGCCTCAGAAACAAAAGGTAGAGGCTGAAC5140.6877082190497852No Hit
CATAGATGAAAGAGCATTGGTTGTGACACCGAAAAACACCCTCTATGTGT5130.6863702653162254No Hit
CATAGAGGTGTTTCTTATAGTCAACTGAAGTACGGTAGCAGAGGCAAGGG5100.6823564041155457No Hit
CATAGAACTGAAACTAGGAAACATGTCTGAAACATTTTCTGGTCAGCTGT4140.5539128456937958No Hit
CATAGAGTTCTCACTCTCCGCTAATTCTGTGTGGCCAACAGGAAGGTCTA3780.5057465112856397No Hit
CATAGATCCCTGGCAAGAATTCCTCTTCTTCCTCCTCCTGTCTATGTGTA3700.4950428814171606No Hit
CATAGATCCAGCTTCATCCATGTCCCGTGGTGTGCAAAATTCTCTTGACA3430.45891813061104353No Hit
CATAGAAAGGGACAAGGGCTCACTCTGCTGCTCACTCTTTGGGTTCTATG3370.45089040820968407No Hit
CATAGAGATCTTCTCGGCTTAGCGGCCATTGTCGCCTTCTCTTCTCTATG3300.4415247320747649No Hit
CATAGAGCCATCATCATTTCTCACCCAGGGTAATTCTGGCCTTGTTCTAT3220.43082110220628567No Hit
CATAGACTCACTCTGTCACCCAAGCTGGAGTGCAGTGGCGTGCTCTTGGC3220.43082110220628567No Hit
CATAGAGTTCTCACTCTCCGCTAATTCTCCAACCTGTGTATATTCTCCTG3180.4254692872720461No Hit
CATAGAGGTGTTTCTTATAGTCAACTGAAGTACACTGAAACATTTTCTGG3020.40406202753508785No Hit
CATAGAACAATCAGGCACCCACCATGTCGTCAGTGTAACCTGTGTATATT2980.39871021260084827No Hit
CATAGATCTCAAACAGCCTCTCCCAGAAGCTTTGAGATTTGTGTCTGTTC2480.33181252592285354No Hit
CATAGAACAATCAGGCACCCACCATGTCGTCAGTGTAGTATGCAGTCTGT2400.3211088960543744No Hit
CATAGACTCACTCTGTCACCCAAGCTGGAGTGCAATGGCATGATCTCAGC2150.2876600527153771No Hit
CATAGACAAGCCTGCCAAGACCTCTCTACGGAGGCTGAAACTCTAAGGGT2110.2823082377811375No Hit
CATAGATAGCAGCAATCTCAGGCCTGGGAGACCCCATATCTACCAAAGAT2070.2769564228468979No Hit
CATAGAACAATCAGGCACCCACCATGTCGTCAGTGTGAGGTTTCCTGGGC1940.25956302431061934No Hit
CATAGATCAAGGTGTGAGCATGGTCCCGTGGTGTGCAAAATTCTCTTGAC1920.2568871168434995No Hit
CATAGATGTTGTTGTTCTGTTGCCCACTCTTTGGGTTCTATGTGTAGAAC1850.2475214407085803No Hit
CATAGACTCACTCTGTCACCCAAGCTGGAGTGCAAGCGGCACGGTCCCGG1790.23949371830722094No Hit
CATAGAGGGCTATCCTTGCTCAGATCACATTTATCGGGAGCCACAGATCT1600.21407259736958298No Hit
CATAGACGGGAAGAGTAAGACCACCCTCTATGACATTTATCGGGAGCCAC1470.19667919883330434No Hit
CATAGAGCTAGATCTAATTCTGTATGAGCACAGGAAAGTTGGAACCTATC1460.19534124509974446No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCCTCAGGTCA1420.18998943016550487No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCGGGTCA1420.18998943016550487No Hit
CATAGAGTTCTCACTCTCCGCTAATTCTACACTATGGCTTAATGTTTCTC1360.18196170776414552No Hit
CATAGAAAGGGACAAGGGCTCACTCTCTGCTGCTCACTCTTTGGGTTCTA1350.18062375403058564No Hit
CATAGAGGTGAGCGGCTGTCTCCACAGGTCCGCGCAGAGCCTTCAGGTCA1280.17125807789566638No Hit
CATAGAGGTGAGCGGCTGTCTCCAAGTCCGCGCAGAGCCTTCAGGTCAGA1270.16992012416210647No Hit
CATAGAGGTGTTTCTTATAGTCAACTGAAGTCCCATGATCTATGTGTAGA1250.16724421669498668No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCG1230.16456830922786692No Hit
CATAGACTCACTCTGTCACCCAAGCTACACTGACACTATGGCTTAATGTT1220.163230355494307No Hit
CATAGAAAGGGACAAGGGCTCACTCTTTGGGTTCTGCTGCTCACTCTTTG1190.15921649429362733No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTCCAGGTCA1150.15386467935938775No Hit
CATAGATAGCAGCAATCTCAGGCCTCTCTTCCCTCCTCTCCTCGTCTTCT1110.1485128644251482No Hit
CATAGATGTTGTTGTTCTGTTGCCCATACGGTAGCAGAAGCTTTGAGATT1100.14717491069158828No Hit
CATAGAGGTGAGCGGCTGTCTCCACGAGTCCGCGCAGAGCCTTCAGGTCA1090.1458369569580284No Hit
CATAGATCTAGCTTCATCCATGTCCCGTGGTGTGCAAATTCTCTATGTGT1040.13914718829022893No Hit
CATAGAAAGGGACAAGGGCGCACTCTAGCCTGGGAGACATCTATGTGTAG1020.13647128082310914No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGCCA1010.13513332708954923No Hit
CATAGAACAATCAGGCACCCACCATGTCGTCAGTGCAGGAAAGTTGGAAC980.13111946588886958No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTGCAGGTCA980.13111946588886958No Hit
CATAGATCTCAAACAGCCTCTCCCAGTCACAGGGACAAGGGAATGATGGT970.12978151215530967No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCGGAGCCTTCAGGTCA950.12710560468818988No Hit
CATAGATGTTGTTGTTCTGTTGCCCAGATCTATGTGTAGAACCATGTCGT950.12710560468818988No Hit
CATAGAAAGGGACAAGGGCTCACTCTTTGGGCTGCTGCTCACTCTTTGGG920.1230917434875102No Hit
CATAGAACAATCAGGCACCCACCATGTCGTCAGTGTAGTGCCATACCAAG920.1230917434875102No Hit
CATAGAGGTGAGCGGCTGTCTCCATAAGTCCGCGCAGAGCCTTCAGGTCA900.12041583602039041No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGGGCCTTCAGGTCA880.11773992855327062No Hit
CGTAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA880.11773992855327062No Hit
CATAGAGGTGAGCGGCTGTCCCCACAAGTCCGCGCAGAGCCTTCAGGTCA810.10837425241835139No Hit
CATAGAGCTAGATCTAATTCTGTATGAGCACAGGGTAATTCTGGCCTTGT810.10837425241835139No Hit
CATAGATCCAGCTTCATCCATGTCCCGTGGTGTGCAAATTCTCTATGTGC810.10837425241835139No Hit
CATAGAACTGAAACTAGGAAACATGTCTGAGGCTGAAACTCTAAGGGTCT790.1056983449512316No Hit
CATAGAAAGGGACAAGGGCTCACTCTTTGGGTTCTATGTGTAGAACTGCT780.1043603912176717No Hit
CATAGATCCAGCTTCATCCATGTCCCTACAAAGGACATGAACTCATCATG780.1043603912176717No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGCGCCTTCAGGTCA770.1030224374841118No Hit
CATAGATGAAAGAGCATTGGTTGTAACACTACGGTAGCAGAGGCAAGGGT770.1030224374841118No Hit
CATAGATCCCTGGCAAGAATTCCTCAATATGAACAGAAAGAAGCTCTATG760.10168448375055189No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)