FastQCFastQC Report
Tue 7 May 2024
EGAF00005806553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005806553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49991
Total Bases7.4 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCTGCG2756755.143925906663206No Hit
CATAGAGAATTTGCACACCACGGGACATGGATGAAGCTGGATCTATGAGA19043.8086855634014123No Hit
CATAGATGTCTCCCAGGCTAGAGTGAGCCCTTGTCCCTTTCTATGAGACC11442.2884119141445463No Hit
CATAGATCATGGGACTTCAGCATGGTGGGTGCCTGATTGTTCTATGAGAC4790.9581724710447881No Hit
CATAGACCACACCCAAACACCACGTACACTGACGACATGGTTCTACACAT4510.9021623892300614No Hit
CATAGAGAATTTGCACACCACGGGACCATGCTCACACCTTGATCTATGAG4450.8901602288411914No Hit
CATAGAACCCAAAGAGTGAGCAGCAGAATCAGGGTTGGTTCTATGAGACC4170.8341501470264647No Hit
CATAGAACCCAAAGAGTGAGCAGCAGCACTGACGACATGGTTCTACACAT3750.7501350243043747No Hit
CATAGACACTGATAGGTTCCAACTTTCCTGGGAGAGGCTGTTTGAGATCT3490.6981256626192714No Hit
CATAGACTTGATGCCCAGGAAACCTCTATGAGACACTTCAGTTGACTATA3000.6001080194434998No Hit
CATAGAGCTTCTTTCTGTTCATATTGGGCAACAGAACAACAACATCTATG2430.4860874957492349No Hit
CATAGAACCCAAAGAGTGAGCAGCAGAGTGAGCCCTTGTCCCTTTCTATG2350.4700846152307416No Hit
CATAGAACAGACACAAATCTCAAAGCTTCTACACTGACGACATGGGCAAC2340.46808425516592983No Hit
CATAGATTAGTTCTTATTCTTGGTATGGCACGAACACTGACGACATGGTG2100.4200756136104499No Hit
CATAGACACTGATAGGTTCCAACTTTCCTGACACTGACGACATGGTGGGT2070.41407453341601486No Hit
CATAGAGCTTCTTTCTGTTCATATTGGGGAACAGAACAACAACATCTATG1860.3720669720549699No Hit
CATAGAGGGTGTTTTTCGGTGTTACAACCAATGCTCTTTCATCTATGAGA1860.3720669720549699No Hit
CATAGACCTTCCTGTTGGCCACACAGAATTAGCGGAGAGTGAGAACTCTA1740.3480626512772299No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTCTGCAGATTTGGACCTGCG1730.3460622912124182No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCGGATTTGGACCTGCG1670.33406013082354824No Hit
CATAGACACTGATAGGTTCCAACTTTCCTGGGCAACAGAACAACAACATC1560.3120561701106199No Hit
CATAGAACTATGTCAAGAGAATTTTGCACACCACGGGACATGGATGAAGC1480.29605328959212657No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTCGCAGATTTGGACCTGCG1440.2880518493328799No Hit
CATAGACTTGATGCCCAGGAAACCTCACACTGACGACATGGTGGGTGCCT1430.2860514892680683No Hit
CATAGACAGCTGACCAGAAAATGTTTCAGTGTGCCGCTAAGCCGAGAAGA1380.27604968894400994No Hit
CATAGATTAGTTCTTATTCTTGGTATGGCACTATGCTGCTTTTCTCTTCT1350.2700486087495749No Hit
CATAGAACAAGGCCAGAATTACCCTGGGAGAGGCTGTTTGAGATCTATGA1290.2580464483607049No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGACTTGGACCTGCG1260.25204536816626993No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCCGCG1260.25204536816626993No Hit
CATAGAACCCAAAGAGTGAGCAGCAGCGGTGAGGAAGCTTCCTGAGCTGT1250.25004500810145824No Hit
CATAGATCATGGGACTTCAGCATGGCGGCGTTTGCAGATTTGGACCTGCG1170.23404212758296492No Hit
CATAGAGGGTGTTTTTCGGTGTCACAACCAATGCTCTTTCATCTATGAGA1160.23204176751815325No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGCTTGCAGATTTGGACCTGCG1120.2240403272589066No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTCGGACCTGCG1120.2240403272589066No Hit
CATAGAGGGTGTTTTTCGGTGTCACAACACTGACGACATGGTGGGTGCCT1090.21803924706447161No Hit
CATAGATCATGGGACTTCAGCATGGTGGTGTTTGCAGATTTGGACCTGCG1090.21803924706447161No Hit
CATAGAACCCAAAGAGTGAGCAGCAGAGAGTGAGCCCTTGTCCCTTTCTA1090.21803924706447161No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGGTTTGGACCTGCG1010.20203636654597826No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGGCCTGCG1000.2000360064811666No Hit
CATAGAACAGACACAAATCTCAAAGCTTCTCTACCCCCAGTATTTCATTA970.1940349262867316No Hit
CATAGAACCCAAAGAGTGAGCAGCAGAACCCAAAGAGTGAGCCCTTGTCC950.19003420615710828No Hit
CATAGAACAGACACAAATCTCAAAGCTTCTACACTGACGACATGGTGGGT940.18803384609229662No Hit
CATAGACAGCTCACAGACTGCATACTACACTGGAGACAGCCGCTCACCTC870.17403132563861495No Hit
CATAGAATGCAGGAGAATATACACAGGTTACACTGACGACATGGTGGGTG830.16602988537936827No Hit
CATAGAACAAGGCCAGAATTACCCTGGAGACAGCCGCTCACCTCTATGAG820.16402952531455664No Hit
CATAGAACAAGGCCAGAATTACCCTGGGTGAGAAATGATGATGGCTCTAT820.16402952531455664No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATCTGGACCTGCG810.16202916524974495No Hit
CATAGAACAAGGCCAGAATTACCCTGGGCAACAGAACAACAACATCTATG810.16202916524974495No Hit
CATAGAACCCAAAGAGTGAGCAGCAGGTTCTACACATAGAACCCAAAGAG790.15802844512012162No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGATCTGCG760.15202736492568664No Hit
CATAGAACCCAAAGAGTGAGCAGCAGACATGGTTCTACACATAGAACCCA750.15002700486087495No Hit
CATAGATCATGGGACTTCAGCATGGCAGTGTTTGCAGATTTGGACCTGCG740.14802664479606328No Hit
CATAGAACCCAAAGAGTGAGCAGCAGCCCAAAGAGTGAGCCCTTGTCCCT720.14402592466643996No Hit
CATAGACAGGAGGAGGAAGAAGAGGATCTATAGGGACATGGATGAAGCTG710.1420255646016283No Hit
CATAGAACCCAAAGAGTGAGCAGCAGCTGACGACATGGTTCTACACATAG690.13802484447200497No Hit
CATAGAACTATGTCAAGAGAATTTTGCACACCACGGGACCATGCTCACAC650.1300234042127583No Hit
CATAGACAGCTCACAGACTGCATACTACACTGGGACATGGATGAAGCTGG630.12602268408313497No Hit
CATAGATTCTCCCTTGTGCTTTTGGTCCCTTGTGCTTTTGGTCCCTTGTG620.1240223240183233No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGTAGATTTGGACCTGCG610.12202196395351163No Hit
CATAGAACCCAAAGAGTGAGCAGCAGTTCTACACATAGAACCCAAAGAGT600.12002160388869995No Hit
CATAGAGGGTGTTTTTCGGTGTCACACTGACGACATGGTTCTACACATAG580.11602088375907663No Hit
CGTAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCTGCG570.11402052369426498No Hit
TAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCTGCGAG560.1120201636294533No Hit
CATAGATCTGTGGCTCCCGATAAATGTGATCTGAGCAAGGATAGCCCTCT560.1120201636294533No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCTGTG550.11001980356464162No Hit
CATAGATCATGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCTGCGA550.11001980356464162No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGTTTTGGACCTGCG550.11001980356464162No Hit
CATAGATGTCTCCCAGGCTAGAGTGCAATCAAATTCGAACTCAGGATTGG540.10801944349982998No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTTGACCTGCG540.10801944349982998No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGTACCTGCG530.1060190834350183No Hit
CATAGATCATGGGACTTCAGCATGGCGGTATTTGCAGATTTGGACCTGCG520.10401872337020664No Hit
CATAGAACCCAAAGAGTGAGCAGCAGGCAGTCTCAAACGTTGTGTCTATG520.10401872337020664No Hit
CATAGACTTGATGCCCAGGAAACCTCCTGCCCCTGCAGGCTGAAAGTGCC510.10201836330539497No Hit
TATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCTGCG510.10201836330539497No Hit
CATAGAACAGACACAAATCTCAAAGCTTCTCTATGAGACCAAGTCACTTC510.10201836330539497No Hit
CATAGAACCCAAAGAGTGAGCAGCAGACGACATGGTTCTACACATAGAAC510.10201836330539497No Hit
CATAGATGTCTCCCAGGCTAGAGTGCGCCCTTGTCCCTTTCTATGAGACC510.10201836330539497No Hit
CATAGATTCTCCCTTGTGCTTTTGGTACACTGACGACATGGTGGGTGCCT500.1000180032405833No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)