FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005806614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005806614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119433
Sequences flagged as poor quality0
Sequence length150
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNTAGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA68305.718687464938501No Hit
NNNAGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA66025.527785452931769No Hit
NNNNGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA65005.442381921244547No Hit
NATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA62905.266551120712031No Hit
NNTNGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA50614.237522292833639No Hit
NNNNGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA17771.4878634883156245No Hit
NNNAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA16951.4192057471553088No Hit
NNNAGNGGTGAGCGGNTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA16471.3790158498907337No Hit
NNTAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA16271.3622700593638273No Hit
NNTAGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA15401.2894258705717851No Hit
NNNAGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA15371.2869140019927492No Hit
NNNNGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA14171.1864392588313113No Hit
NATAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA13741.1504358091984628No Hit
NNTNGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA11500.9628829552971122No Hit
NATAGAGGTGAGCGGNTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA11460.9595337971917309No Hit
NNNAGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT6550.5484246397561813No Hit
NNTAGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT6270.5249805330185124No Hit
NNNNGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT5850.48981437291200924No Hit
NATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT5610.46971942427972174No Hit
NNNAGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT5200.4353905536995638No Hit
NNNNGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT5010.4194820526990028No Hit
NNTAGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT4970.4161328945936215No Hit
NNTNGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT4560.3818040240134636No Hit
NATAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT4080.34161412674888847No Hit
NNNNGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA4020.3365903895908166No Hit
NNNAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA3820.31984459906391033No Hit
NNTAGNCTGCCTCAGCCTCCTGAGTAGGTCAGGAGATTGAGACCATCCTG3790.31733273048487437No Hit
NNTAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA3780.31649544095852905No Hit
NNTNGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT3760.31482086190583847No Hit
NNNAGNCTGCCTCAGCCTCCTGAGTAGGTCAGGAGATTGAGACCATCCTG3710.31063441427411187No Hit
NATAGACTGCCTCAGCCTCCTGAGTAGGTCAGGAGATTGAGACCATCCTG3630.3039360980633493No Hit
NNNNGNCTGCCTCAGCCTCCTGAGTAGGTCAGGAGATTGAGACCATCCTG3490.2922140446945149No Hit
NNNAGNATCCTTTCCNCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA3430.28719030753644303No Hit
NNNAGNTTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT3380.28300385990471644No Hit
NNTAGNTTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT3130.26207162174608356No Hit
NNTNGNCTGCCTCAGCCTCCTGAGTAGGTCAGGAGATTGAGACCATCCTG2950.24700041027186792No Hit
NNNNGNTTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT2930.24532583121917728No Hit
NNNAGNTTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA2890.24197667311379603No Hit
NNTAGNTTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA2850.23862751500841475No Hit
NATAGATTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT2780.23276648832399757No Hit
NNNAGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGAAAG2620.2193698559024725No Hit
NNTAGNTTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA2610.21853256637612722No Hit
NNNNGNTTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA2600.2176952768497819No Hit
NNTNGNTTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT2530.21183425016536467No Hit
NNNNGNTTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA2440.20429864442825682No Hit
NNNNGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGAAAG2270.19006472248038647No Hit
NNNAGNTTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA2260.18922743295404118No Hit
NNTAGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGAAAG2190.18336640626962397No Hit
NNTNGNTTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA2180.18252911674327865No Hit
NATAGATTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA2160.18085453769058804No Hit
NATAGAATCCTTTCCNCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA2090.17499351100617083No Hit
NATAGATTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA2080.1741562214798255No Hit
NATAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGAAAG2080.1741562214798255No Hit
NNNAGNCTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA1970.16494603669002705No Hit
NNTNGNTTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA1940.1624341681109911No Hit
NNTAGNCTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA1880.15741043095291923No Hit
NNNAGNCCTCCTGTTTGACCATTTTGAACATAGCCTTTGGGGTTTCTATG1830.15322398332119264No Hit
NNTNGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGAAAG1830.15322398332119264No Hit
NATAGACTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA1810.151549404268502No Hit
NNNNGNCTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA1790.14987482521581136No Hit
NNTAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT1680.14066464042601293No Hit
NNNAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT1650.13815277184697697No Hit
NNTAGNCCTCCTGTTTGACCATTTTGAACATAGCCTTTGGGGTTTCTATG1640.13731548232063165No Hit
NNTAGNAAGGCCTTCCTGTATCAGTCATCACTTATACGCCTCCCTGTCTA1610.1348036137415957No Hit
NNTAGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGTGCCAC1610.1348036137415957No Hit
NNNNGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT1600.13396632421525037No Hit
NNNAGNCTGCCTCAGNCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT1520.12726800800448787No Hit
NNNAGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGTGCCAC1510.12643071847814255No Hit
NNNAGNAGTTCTGATCCACATTCACGTATAGCCCCTAAGCCCAGGTCTAT1450.12140698132007068No Hit
NNTNGNCTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA1440.12056969179372536No Hit
NATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGTGCCAC1440.12056969179372536No Hit
NNNNGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGTGCCAC1440.12056969179372536No Hit
NNNNGNCCTCCTGTTTGACCATTTTGAACATAGCCTTTGGGGTTTCTATG1390.11638324416199879No Hit
NNTAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT1380.11554595463565347No Hit
NATAGACCTCCTGTTTGACCATTTTGAACATAGCCTTTGGGGTTTCTATG1380.11554595463565347No Hit
NNNNGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT1370.11470866510930815No Hit
NNNAGNATCCTTTCCNCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT1360.11387137558296283No Hit
NNNNGNAAGGCCTTCCTGTATCAGTCATCACTTATACGCCTCCCTGTCTA1350.11303408605661752No Hit
NNNAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT1340.1121967965302722No Hit
NNTAGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCAACATGGTG1330.11135950700392688No Hit
NNTAGNAGTTCTGATCCACATTCACGTATAGCCCCTAAGCCCAGGTCTAT1320.11052221747758156No Hit
NNNAGNAAGGCCTTCCTGTATCAGTCAGTGTATGCAAGACTAGGCAATTT1310.10968492795123624No Hit
NATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCAACATGGTG1310.10968492795123624No Hit
NNTAGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGTGCACC1290.10801034889854563No Hit
NNNAGNATCCTTTCCCCATTGCTTGCTCATTATTATTGGATGGGATCTAT1270.106335769845855No Hit
NNTNGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGTGCCAC1260.10549848031950969No Hit
NNTAGNATCCTTTCCCCATTGCTTGCTCATTATTATTGGATGGGATCTAT1260.10549848031950969No Hit
NNNAGNAAGGCCTTCCTGTATCAGTCATCACTTATACGCCTCCCTGTCTA1260.10549848031950969No Hit
NNNAGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCAACATGGTG1210.10131203268778312No Hit
NNNAGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGTGCACC1210.10131203268778312No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)