FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005806725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005806725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198500
Sequences flagged as poor quality0
Sequence length150
%GC35

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGAGCTTGGGTGCGAACATCAAGGAAGTTTCTGTGAGATCTATGAGA191439.64382871536524No Hit
CATAGACGGATTATTCTCTCCATGGTCTACCAGTTATTAGTTGAATGAGC138326.968261964735517No Hit
CATAGATGTTTTATCAAAGGCATGCTCATTTATTTTAAAGGACTGGAAGC126866.390931989924434No Hit
CATAGAGCTTTTTCTCCAGCCTCCAAGTAATAACCAGGCCCCTCTATGAG124536.2735516372795965No Hit
CATAGAGAAGACTCCCTCAACCCACCCACAGCCAGGAAAGAATCTATGAG111765.630226700251889No Hit
CATAGAAAATTTGTGATGGTAAATCATGGGACATGCCAGGAATCAATGTT82504.156171284634761No Hit
CATAGAAATGATGCTGAAGGGCCTGCCCTCTCTGCTGTTCCTTCTATGAG78283.9435768261964737No Hit
CATAGATCATGGGACTTCAGCATGGCTCAGGTATGTCTTTATTAGCTCTA77303.8942065491183877No Hit
CATAGATCACGTAGAAACTGGAGAGGCTAATCTGAAATCTTCGTGTTCTA70773.5652392947103277No Hit
CATAGATGTTTTATCAAAGGCATGCTCATCAAGGAAGTTTCTGTGAGATC62233.135012594458438No Hit
CATAGAGGAGGTGAAAGAAATCTACAATGGGTTCTGGGCCCCAGTGTGAA54312.736020151133501No Hit
CATAGACTCATTGCACTTTCCACAACTGTGTGCTTGAACCAGATTCTATG52122.6256926952141058No Hit
CATAGACTCATTGCACTTTCCACAACTGTCCCAACTGTCTCTGAAAAATT47892.4125944584382872No Hit
CATAGAAGTCACGAGTTTTGTTGTTGTTCTTCCTTTTCTGCCTTCATCTA47872.411586901763224No Hit
CATAGACTCATTGCACTTTCCACAACTGTCATTGTCGCTGTCCCATCTAT38551.942065491183879No Hit
CATAGATCAAGGAAAACACCAGCCCACGCTGTCCTTCTCTGAATCTATGA31711.5974811083123426No Hit
CATAGAAATGATGCTGAAGGGCCTGCTGAAGTGATGGGCTAGATCTATGA28701.4458438287153652No Hit
CATAGAAATGATGCTGAAGGGCCTGTTTCCCCACTCAAATACCATCTATG23231.1702770780856422No Hit
CATAGACCTAAAAGGCTATTGAAAGGAAGGACATGCCAGGAATCAATGTT22581.1375314861460957No Hit
CATAGAAGGACAAAGACATTTAGGACCCTGCAGTCTTTGGAGGAATCTAT21721.0942065491183879No Hit
CATAGAGGAGGTGAAAGAAATCTACAATGGGCCCCAGTGTGAATACTTCT17030.857934508816121No Hit
CATAGAGTGCCTGTTTCCTCATTCGTCTGTTTCCCCACTCAAATACCATC15610.7863979848866498No Hit
CATAGAACAGATGTTCTCCTTGTTTTCTATAACATAGAGATCCCACCATT15330.7722921914357682No Hit
CATAGAAGCTGAGAAGACTTCCTAATGCAGAAGTAGGAAGTATTACCCCT10370.5224181360201512No Hit
CATAGAGCTTTTTCTCCAGCCTCCAAGGGGTAAGGCGTACTGGTCTATGA10080.507808564231738No Hit
CATAGAGTCTGAATGGTGGGAGAGTGAAAGAAATCTACAAAGGATTCTGG9930.5002518891687657No Hit
CATAGACCTCCGACAGTTTTTATGCACCTTCATTGTTGCTCTATGAGACC9010.4539042821158691No Hit
CATAGATGTGTAGCCTGGGTCTGACATGAGGAGCTGAGTCATGGGGTCTA8550.4307304785894206No Hit
CATAGATGTTTTATCAAAGGCATGCTCATCAGGAACCATGGGAGGTCTAT8400.42317380352644834No Hit
CATAGACTCATTGCACTTTCCACAACTGTCTGCCTCTAAGTAAACTGAAT7410.3732997481108313No Hit
CATAGAAATGATGCTGAAGGGCCTGCTGCTGAAGTGATGGGCTAGATCTA7200.36272040302267No Hit
CATAGACACAACAGAGAGAACGGGCTACACTGACGCTATACCACACTACA6370.3209068010075567No Hit
CATAGACCAGCAGACACTCCCATCATTTATTTTAAAGGACTGGAAGCCTC5880.29622166246851384No Hit
CATAGACCAGCAGACACTCCCATCATCAGGAACCATGGGAGGTCTATGAG5670.2856423173803527No Hit
CATAGAGCTTGGGCGCGAACATCAAGGAAGTTTCTGTGAGATCTATGAGA5640.2841309823677582No Hit
CATAGACCTCCGACAGTTTTTATGCACCCACAGCCAGGAAAGAATCTATG5380.2710327455919395No Hit
CATAGACCAACATTTCTTCTCCCAGGGTCTGTTTCCCCACTCAAATACCA4790.24130982367758189No Hit
CATAGACCTCCGACAGTTTTTATGCACTGTCCCAACTGTCTCTGAAAAAT4720.23778337531486143No Hit
CATAGATGTGTAGCCTGGGTCTGACATGCCAGGAATCAATGTTCTATGAG4290.21612090680100757No Hit
CATAGACGGATTACTCTCTCCATGGTCTACCAGTTATTAGTTGAATGAGC4050.2040302267002519No Hit
CATAGATCAAGGAAAACACCAGCCCACCCACAGCCAGGAAAGAATCTATG4000.20151133501259444No Hit
CATAGACCAGCAGACACTCCCATCATACACTGACGCTATACCACACTACA3910.19697732997481107No Hit
CATAGAGCTGTACTGCATCTTTTCTCAAACTGCTGGGGTTGCTCTATGAG3650.18387909319899243No Hit
CATAGACTCATTGCACTTTCCACAACTGTACACTGACGCTATACCACACT3530.1778337531486146No Hit
CATAGATGTTTTACCAAAGGCATGCTCATTTATTTTAAAGGACTGGAAGC3460.1743073047858942No Hit
CATAGAGCTTTTTTTCCAGCCTCCAAGTAATAACCAGGCCCCTCTATGAG3440.17329974811083124No Hit
CATAGACGTGACTAAAAGCATGTTGGTGGAAGGGTAAGAGAGGGTCTATG3430.17279596977329975No Hit
CATAGAAAGGTGCAATCAAGCCAGTTGGCTCAGGTATGTCTTTATTAGCT3280.16523929471032747No Hit
CATAGATGTTTTATCAAAGGCATGCTCATTGCTTCTTGTGGTAACTGGTC3270.16473551637279596No Hit
CATAGACACAACAGAGAGAACGGGCTACACTGACGACATGGTTCTACACA3230.16272040302267002No Hit
CATAGATGGCCAAGATACGAGCACATAGAGATCCCACCATTTGCCATCTA3210.16171284634760705No Hit
CATAGAGAAGACTTCCTCAACCCACCCACAGCCAGGAAAGAATCTATGAG3160.15919395465994962No Hit
CATAGACCGCTTCCCAATTTTTGTAACACACTGACGCTATACCACACTAC3090.15566750629722922No Hit
CATAGAAATGATGCTGAAGTGATGGGCTAGATCTATGAGACCAAGTCTCT2980.15012594458438286No Hit
CATAGAGTCCAACTATTCCAGCACCACGCTGTCCTTCTCTGAATCTATGA2660.13400503778337533No Hit
CATAGAAATGATGCTGAAGGGCCTGCTGATGCTGAAGTGATGGGCTAGAT2630.13249370277078085No Hit
CATAGAAATGATGCTGAAGGGCCTGCATAGAAATGATGCTGAAGTGATGG2620.13198992443324936No Hit
CATAGAGAAGACTCCCTCAACCCACGCTGTCCTTCTCTGAATCTATGAGA2540.12795969773299748No Hit
CATAGAAATGATGCTGAAGGGCCTGCGATGCTGAAGTGATGGGCTAGATC2500.12594458438287154No Hit
CATAGACTCATTGCACTTTCCACAACTGTCTCTGTCCCAACTGTCTCTGA2380.11989924433249369No Hit
CATAGAAATGATGTTGAAGGGCCTGCCCTCTCTGCTGTTCCTTCTATGAG2330.11738035264483627No Hit
CATAGAGTCTGAATGGTGGGAGAGTGATGCTGAAGTGATGGGCTAGATCT2290.11536523929471032No Hit
CATAGAAAATTTGCGATGGTAAATCATGGGACATGCCAGGAATCAATGTT2280.11486146095717885No Hit
CATAGACGTGACTAAAAGCATGTTGGTGCATTACAGAGAGGAGATCTATG2270.11435768261964735No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCTGCG2260.11385390428211588No Hit
CATAGAAATGATGCTGAAGGGCCTGATGCTGAAGTGATGGGCTAGATCTA2100.10579345088161209No Hit
CATAGACCTCCGACAGTTTTTATGCACTGTCATTGTCGCTGTCCCATCTA2050.10327455919395467No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)